Fermentation Characteristics of Low Salted Kochujang Prepared with Sub-materials

부원료를 첨가한 저식염 고추장의 발효특성 변화

  • Kim, Dong-Han (Department of Food and Nutrition, Mokpo National University) ;
  • Yang, Sung-Eun (Department of Food and Nutrition, Mokpo National University)
  • 김동한 (목포대학교 생활과학부 식품영양학 전공) ;
  • 양성은 (목포대학교 생활과학부 식품영양학 전공)
  • Published : 2004.02.28


Effects of sub-materials such as, ethanol, mustard, and chitosan, on enzymatic, microbial and physicochemical characteristics of kochujang were investigated during fermentation. Activity of ${\alpha}$-amylase was low in chitosan-added kochujang, whereas those of ${\beta}$-amylase and pretense did not show any remarkable difference. Viable cells of yeast and bacteria decreased in sub-material-added kochujang during fermentation, with yeast counts decreasing more rapidly in ethanol- and mustard-added kochujang than that with chitosan. Consistency of kochujang decreased during fermentation, with the highest consistency observed in ethanol-added kochujang. Oxidation-reduction potential was low in chitosan-added kochujang. Water activity of all kochujang groups decreased during fermentation with the lowest in ethanol-added kochujang. Hunter L-, a-, and b-values of chitosan-added kochujang were higher than other groups, whereas increase in total color difference of low-salt without sub-material group was lowest pH of kochujang was the highest in ethanol-added kochujang, whereas titratable acidity increased remarkably in chitosan-added group. Total sugar and reducing sugar contents of kochujang were high in ethanol-and mustard-added groups, whereas ethanol production decreased remarkably in mustard-added kochujang. Amino nitrogen content was highest in low-salt without sub-material kochujang during late aging period. Ammonia nitrogen content was lower in mustard-added kochujang. After 12 weeks of fermentation, ethanol-added kochujang was more acceptable than mustard-and chitosan-added groups in taste, color, and overall acceptabilities.


kochujang;low-salt;ethanol;mustard;chitosan;physicochemical properties


  1. Cho HO, Park SA, Kim JG. Effect of traditional and improved kochujang koji on the quality improvement of traditional kochujang. Korean J. Food Sci. Technol. 13: 319-327 (1981)
  2. Kwan DJ, Jung JW, Kim JH, Park JY, Yoo JY, Koo YJ, Chung KS. Studies on establishment of optimal aging time of Korean traditional kochujang. Agric. Chem. Biotech. 39: 127-133 (1996)
  3. Kim YS, Cha J, Jung SW, Park EJ, Kim JO. Changes of physicochemical characteristics and development of new quality indices for industry-produced koji kochujang. Korean J. Food Sci. Technol. 26: 453-458 (1994)
  4. Shin DH, Kim DH, Choi U, Lim MS, An EY. Physicochemical characteristics of traditional kochujang prepared with various raw materials. Korean J. Food Sci. Technol. 29: 907-912 (1997)
  5. Jung SW, Kim YH, Koo MS, Shin DB. Changes in physicochemical properties of industry-type kochujang during storage. Korean J. Food Sci. Technol. 26: 403-410 (1994)
  6. Chun MS, Lee TS, Noh BS. Effect of gamma-irradiation on quality of kochujang during storage. Foods Biotechnol. 1: 117-122 (1992)
  7. Lee KY, Kim HS, Lee HG, Han O, Chang UJ. Studies on the prediction of the shelf-life of kochujang through the physicochemical and sensory analyses during storage. J. Korean Soc. Food Sci. Nutr. 26: 588-594 (1997)
  8. Lee KS, Kim DH. Effect of sake cake on the quality of low salted kochujang. Korean J. Food Sci. Technol. 23: 109-115 (1991)
  9. Yamamoto Y, Higashi K, Yoshii H. Inhibitory activity of ethanol on food spoilage bacteria. Nippon Shokuhin Kogyo Gakkaishi 31: 531-535 (1984) https://doi.org/10.3136/nskkk1962.31.8_531
  10. Kim DH, Lee JS. Effect of condiments on the physicochemical characteristics of traditional kochujang during fermentation. Korean J. Food Sci. Technol. 33: 353-360 (2001)
  11. IMT. Official Methods of Miso Analysis. Institute of Miso Technologists, Tokyo, Japan. pp. 1-34 (1968)
  12. Martin EP. Use of acid, rose bengal, and streptomycin in the plate method for estimating soil fungi. Soil Sci. 69: 215-232 (1965)
  13. Jay JM. Modern Food Microbiology, 6th ed. Kluwer Academic/Plenum Publishers, New York, NY, USA. pp. 45-47 (2000)
  14. Oh JY, Kim YS, Shin DH. Changes in physicochemical characteristics of low-salted kochujang with natural preservatives during fermentation. Korean J. Food Sci. Technol. 34: 835-841 (2002)
  15. Shin DH, Ahn EY, Kim YS, Oh JA. Fermentation characteristics of kochujang containing horseradish or mustard. Korean J. Food Sci. Technol. 32: 1350-1357 (2000)
  16. Lee TS. Studies on the brewing of kochujang (red papper paste) by the addition of yeasts. J. Korean Agric. Chem. Soc. 22: 65-90 (1979)
  17. Difco Laboratories. Difico Manual. 19th ed. Difico, Detroit, MI, USA. pp. 1064-1065 (1965)
  18. Shim KH, Seo KI, Kang KS, Moon JS, Kim HC. Antimicrobial substances of distilled components from mustard seed. J. Korean Soc. Food Nutr. 24: 948-955 (1995)
  19. SAS Institute, Inc. SAS User's Guide. Statistical Analysis Systems Institute, Cary, NC, USA (1992)
  20. Moon TW, Kim ZU. Some chemical physical characteristics and acceptability of kochujang from various starch sources. J. Korean Agric. Chem. Soc. 31: 387-393 (1988)
  21. Yun YS, Kim KS, Lee YN. Antibacterial and antifungal effect of chitosan. J. Chitin Chitosan 4: 8-14 (1999)
  22. Fuwa HA. A new method for microdetermination of amylase activity by the use of amylose as the substrate. J. Biochem. 41: 583-588 (1954) https://doi.org/10.1093/oxfordjournals.jbchem.a126476
  23. Choi JY, Lee TS, Noh BS. Quality characteristics of the kochujang prepared with mixture of meju and koji during fermentation. Korean J. Food Sci. Technol. 32: 125-131 (1998)
  24. Anson ML. Estimation of pepsin, trypsin, papain and cathepsin with hemoglobin. J. Gen. Physiol. 22: 79-89 (1938) https://doi.org/10.1085/jgp.22.1.79
  25. Yeo YK, Kim ZU. Studies on the standardization of the processing conditional of Ko-Choo-Jang(red pepper paste). J. Korean Agric. Chem. Soc. 21: 16-21 (1978)
  26. Kim MS, Ahn EY, Ahn YS, Shin DH. Characteristic change of kochujang by heat treatment. Korean J. Food Sci. Technol. 32: 867-874 (2000)
  27. Kim GT, Hwang YI, Lim SI, Lee DS. Carbon dioxide production and quality changes in Korean fermented soybean paste and hot pepper-soybean paste. J. Korean Soc. Food Sci. Nutr. 29: 807-813 (2000)
  28. Kim DH, Kwon YM. Effect of storage conditions on the microbiological and physicochemical characteristics of traditional kochujang. Korean J. Food. Sci. Technol. 33: 589-595 (2001)
  29. Jung YC, Choi WJ, Oh NS, Han MS. Distribution and physiological characteristics of yeasts in traditional and commercial kochujang. Korean J. Food Sci. Technol. 28: 253-259 (1996)
  30. Lee KS, Kim DH. Trial manufacture of low-salted kochujang (red pepper soybean paste) by the addition of alcohol. Korean J. Food Sci. Technol. 17: 146-154 (1985)
  31. Cho HO, Kim JG, Lee HJ, Kang JH, Lee TS. Brewing method and composition of traditional kochujang (red pepper paste) in junrabook-do area. J. Korean Agric. Chem. Soc. 24: 21-28 (1981)
  32. Kim YS. Studies on the changes in physicochemical characteristics and volatile flavor compounds of traditional kochujang during fermentation. PhD thesis, University of King Sejong, Seoul, Korea (1993)
  33. Thomas YD, Lulvwes WJ, Kraft AA. A convenient surface plate method for bacteriological examination of poultry. J. Food Sci. 46: 1951-1952 (1981) https://doi.org/10.1111/j.1365-2621.1981.tb04528.x
  34. Oh HI, Shon SH, Kim JM. Physicochemical properties of kochujang prepared with Aspergillus oryzae, Bacillus licheniformis and Saccharomyces rouxii during fermentation. J. Korean Soc. Food Sci. Nutr. 29: 357-363 (2000)