In vitro and in vivo Effects of Extracts of Lentinus edodes on Tumor Growth in a Human Papillomavirus 16 Oncogenes-transformed Animal Tumor Model -Apoptosis-mediated Tumor Cell Growth Inhibition-

자궁경부암동물세포에서 표고버섯의 in vitro 및 in vivo 항암효과 -Apoptosis에 의한 종양세포주의 성장억제-

  • Park, Jeong-Min (Department of Medicine, College of Medicine, Catholic University of Daegu) ;
  • Lee, Sung-Hyun (Agriproduct Science Division, National Rural Living Science Institute) ;
  • Kim, Jung-Ok (Department of Medicine, College of Medicine, Catholic University of Daegu) ;
  • Park, Hong-Ju (Agriproduct Science Division, National Rural Living Science Institute) ;
  • Park, Jae-Bok (Department of Medicine, College of Medicine, Catholic University of Daegu) ;
  • Sin, Jeong-Im (Department of Medicine, College of Medicine, Catholic University of Daegu)
  • 박정민 (대구가톨릭대학교 의과대학 의학과) ;
  • 이성현 (농촌생활연구소 농산물가공이용과) ;
  • 김정옥 (대구가톨릭대학교 의과대학 의학과) ;
  • 박홍주 (농촌생활연구소 농산물가공이용과) ;
  • 박재복 (대구가톨릭대학교 의과대학 의학과) ;
  • 신정임 (대구가톨릭대학교 의과대학 의학과)
  • Published : 2004.02.28

Abstract

Fungal products indirectly mediate anti-tumor effects in vitro and in vivo. To investigate whether Lentinus edodes might possess direct anti-tumor substance, L. edodes was extracted and tested on human papillomavirus (HPV) 16 oncogenes-associated animal tumor cells (TC-1) and in an animal tumor model. Only water extract displayed direct anti-proliferative effects in TC-1 tumor cells in vitro. This inhibition was dose-dependent, and inhibitory concentration ($IC_{50}$) was $800\;{\mu}g/mL$. Fungal extracts also showed growth inhibition to human cervical cancer cells (CaSki and HeLa) similarly to TC-1 tumor cells. When fungal extracts were added at a high dose (1.5 mg/mL), cell growth was inhibited within 6 hr following extract treatment. Cell growth inhibition was blocked by heat treatment, but not by low pH, which is indicative of heat sensitivity of this anti-proliferative substance. Cell growth suppression was mediated by apoptosis, as determined by Annexin V and propidium iodide staining. When challenged with TC-1 cells, direct intratumoral injection of fungal extracts resulted in some positive effect on tumor growth inhibition, as compared to oral delivery. Results suggest that heat labile substance of L. edodes suppresses growth of HPV oncogenes-associated tumor cells through apoptosis.

Keywords

Lentinus edodes;apoptosis;anti-tumor activity;human papillomavirus-associated tumor cells

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