Effect of Sigma Factor ${\sigma}^{B}$ on Biofilm Formation of Listeria monocytogenes in High Osmotic and Low Temperature Conditions

고삼투압 및 저온 조건에서 sigma factor ${\sigma}^{B}$가 Listeria monocytogenes biofilm 생성에 미치는 영향

  • Published : 2004.06.30

Abstract

Effects of sigma factor (${\sigma}^{B}$) on biofilm formation in Listeria monocytogenes 10403S and ${\sigma}^{B}-deficient$ sigB null mutant were studied under high osmotic and low temperature conditions. In brain heart infusion (BHI) medium containing 6% NaCl, wild type 10403S and ${\sigma}^{B}-deficient$sigB null mutant formed biofilms of $6.83{\pm}0.38\;and\;5.33{\pm}0.45\;log\;cfu/cm^{2}$, respectively. L. monocytogenes 10403S in BHI medium containing 6% NaCl formed 4.7 times larger biofilm than without NaCl. Culture of L. monocytogenes 10403S and sigB null mutant at $4^{\circ}C$ did not show any significant differences in biofilm formation. The results suggest biofilm formation is activated by ${\sigma}^{B}$ and NaCl, whereas not affected by low temperature stress in L. monocytogenes 10403S. More studies are necessary to determine biofilm formation mechanism in osmotolerant L. monocytogenes.

Keywords

L. monocytogenes;biofilm;osmotolerance;cryotolerance

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