In vitro Bone Marrow Cell Proliferation of Cell Wall Preparation from Bifidobacterium bifidum SL-21

Bifidobacterium bifidum SL-21의 세포벽 조제성분에 의한 in vitro 골수세포 증식활성

  • Shin, Myong-Sook (Department of Food Science and Biotechnology, Kyonggi University) ;
  • Yu, Kwang-Won (Department of Kimchi and Food Science, Chongju National College of Science and Technology) ;
  • Shin, Kwang-Soon (Department of Food Science and Biotechnology, Kyonggi University) ;
  • Lee, Ho (Department of Food Science and Biotechnology, Kyonggi University)
  • 신명숙 (경기대학교 식품생물공학과) ;
  • 유광원 (청주과학대학 김치식품과학과) ;
  • 신광순 (경기대학교 식품생물공학과) ;
  • 이호 (경기대학교 식품생물공학과)
  • Published : 2004.06.30


Bifidobacterium species isolated from infant feces were fractionated into cell wall, cytosol, and extracellular preparations of culture broth, and each fraction was examined for Peyer's patch-mediated bone marrow cell proliferation activity in vitro. Cell wall preparation of B. bifidum SL-21 (CWP) showed the highest bone marrow cell proliferating activity dose dependently, and enhanced production of cytokines, such as hematopoietic growth factor (GM-CSF), IL-2, and IL-6, in culture supernatant of Peyer's patch cells, After treatment with lysozyme, CWP was fractionated, among which intermediate molecular-weight fraction (30-50 kDa) showed significantly high bone marrow cell proliferating activity. These results suggest CWP of B. bifidum SL-21 effectively activates lymphocytes in Peyer's patch, and several cytokines, possibly playing important role in enhancement of systemic immune system, were produced by activated lymphocytes.


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