In vitro Bone Marrow Cell Proliferation of Cell Wall Preparation from Bifidobacterium bifidum SL-21

Bifidobacterium bifidum SL-21의 세포벽 조제성분에 의한 in vitro 골수세포 증식활성

  • Shin, Myong-Sook (Department of Food Science and Biotechnology, Kyonggi University) ;
  • Yu, Kwang-Won (Department of Kimchi and Food Science, Chongju National College of Science and Technology) ;
  • Shin, Kwang-Soon (Department of Food Science and Biotechnology, Kyonggi University) ;
  • Lee, Ho (Department of Food Science and Biotechnology, Kyonggi University)
  • 신명숙 (경기대학교 식품생물공학과) ;
  • 유광원 (청주과학대학 김치식품과학과) ;
  • 신광순 (경기대학교 식품생물공학과) ;
  • 이호 (경기대학교 식품생물공학과)
  • Published : 2004.06.30

Abstract

Bifidobacterium species isolated from infant feces were fractionated into cell wall, cytosol, and extracellular preparations of culture broth, and each fraction was examined for Peyer's patch-mediated bone marrow cell proliferation activity in vitro. Cell wall preparation of B. bifidum SL-21 (CWP) showed the highest bone marrow cell proliferating activity dose dependently, and enhanced production of cytokines, such as hematopoietic growth factor (GM-CSF), IL-2, and IL-6, in culture supernatant of Peyer's patch cells, After treatment with lysozyme, CWP was fractionated, among which intermediate molecular-weight fraction (30-50 kDa) showed significantly high bone marrow cell proliferating activity. These results suggest CWP of B. bifidum SL-21 effectively activates lymphocytes in Peyer's patch, and several cytokines, possibly playing important role in enhancement of systemic immune system, were produced by activated lymphocytes.

Keywords

Bifidobacterium bifidum;bone marrow cell proliferation;Peyer's patch;cell wall preparation;immune system

References

  1. Hopkins MJ, Macfarlane GT. Changes in predominant bacterial populations in human feces with age and with Clostridium difficile infection. J. Med. Microbiol. 51: 448-454 (2002)
  2. Rhee YK, Han MJ, Choi EC, Kim DH. Hypocholesterolemic activity of bifidobacteria isolated from a healthy Korean. Arch. Pharm. Res. 25: 681-684 (2002) https://doi.org/10.1007/BF02976944
  3. Hosono A, Lee J, Ametani A, Natsume M, Hirayama M, Adachi T, Kaminogawa S. Characterization of a water-soluble polysaccharide fraction with immunopotentiating activity from Bifidobacterium adolescentis M101-4. Biosci. Biotechnol. Biochem. 61: 312-316 (1997) https://doi.org/10.1271/bbb.61.312
  4. Catherine M, Marie-Francoise O, Elisabeth S, Marie-Benedicte R, Daniel I. Multiplex PCR using 16S rRNA gene-targeted primers for the identification of bifidobacteria from human origin. FEMS Microbiol. Lett. 222: 129-136 (2003) https://doi.org/10.1016/S0378-1097(03)00245-3
  5. Hong T, Matsumoto T, Kiyohara H, Yamada H. Enhanced production of hematopoietic growth factors through T cell activation in Peyer's patches by oral administration of Kampo(Japanese herbal) medicine 'Juzen-Taiho-To'. Phytomedicine 5: 353-360 (1998) https://doi.org/10.1016/S0944-7113(98)80017-2
  6. Yasui H, Ohwaki M. Enhancement of immune response in Peyer's patch cells cultured with Bifidobacterium breve. J. Dairy Sci. 74: 1187-1195 (1991) https://doi.org/10.3168/jds.S0022-0302(91)78272-6
  7. Charles AJ, Paul T, Mark W, Mark JS. The immune system in health and disease. Part. V. In: Immunobiology. Penelope A, Eleanor L. (eds). Garland Publishing Co., New York, NY, USA (1994)
  8. Mosmann TR, Coffman RL. TH1 and TH2 cells: different patterns of lymphokine secretion lead to different functional properties. Ann. Rev. Immunol. 7: 145-173 (1989) https://doi.org/10.1146/annurev.iy.07.040189.001045
  9. Akio H, Hideaki S, Kazuhiko F, Takeaki M, Joji K, Shigeo I, Woodae K, Hirokazu N. Oral administration of Bifidobacterium longum culture condensate in a diet-restricted murine peritonitis model enhances polymorphonuclear neutrophil recruitment into the local inflammatory site. Nutrition 19: 270-274 (2003) https://doi.org/10.1016/S0899-9007(02)01060-2
  10. Amital H, Gilburd B, Shoenfeld Y. Intelligent nutrition: healthpromoting mechanisms of probiotics. Isr. Med. Assoc. J. 5: 767-769 (2003)
  11. Akiba H, Miyahira Y, Atsuta M, Takeda K, Nohara C, Futagawa T, Matsuda H, Aoki T, Yagita H, Okumura K. Critical contribution of OX40 ligand to T helper cell type 2 differentiation in experimental leishmaniasis. J. Exp. Med. 191: 375-380 (2000) https://doi.org/10.1084/jem.191.2.375
  12. Sakata T, Kojima T, Fujieda M, Takahashi M, Michibata T. Influences of probiotic bacteria on organic acid production by pig caecal bacteria in vitro. Proc. Nutr. Soc. 62: 73-80 (2003) https://doi.org/10.1079/PNS2002211
  13. Laura JF, Glenn RG. Mixed culture fermentation studies on the effects of symbiotics on the human intestinal pathogens Campylobacter jejuni and Escherichia coli. Anaerobe 9: 231-242 (2003) https://doi.org/10.1016/S1075-9964(03)00043-X
  14. He F, Morita H, Hashimoto H, Hosoda M, Kurisaki J, Ouwehand AC, Isolauri E, Benno Y, Salminen S. Intestinal Bifidobacterium species induce varying cytokine production. J. Allergy Clin. Immunol. 109: 1035-1036 (2002) https://doi.org/10.1067/mai.2002.124894
  15. Page B, Page M, Noel C. A new fluorometric assay for cytotoxicity measurements in vitro. Int. J. Oncol. 3: 473-476 (1993)
  16. Shen P, Fan XR, Li GW. The SEM study. pp. 52-63. In: Laboratory Experiments in Microbiology, High Education Press, Beijing, China (1999)
  17. Shin MS, Yu KW, Shin KS, Lee H. Enhancement of immunological activity in mice with oral administration of cell wall components of Bifidobacterium bifidum. Food Sci. Biotechnol. 13: 85-89 (2004)
  18. Lee JN, Lee DY, Ji IH, Kim GE, Kim HN, Sohn J, Kim S, Kim CW. Purification of soluble beta-glucan with immune-enhancing activity from the cell wall of yeast. Biosci, Biotechnol. Biochem. 65: 837-841 (2001) https://doi.org/10.1271/bbb.65.837
  19. Kim JH, Shin KS, Lee H. Characterization and action modde of anti-complementary substance prepared from Lactobacillus plantarum. Korean J. Food Sci. Technol. 34: 290-295 (2002)
  20. Park SY, Ji GE, Ko YT, Jung HK, Ustunol Z, Pestka JJ. Potentiation of hydrogen peroxide, nitric oxide, and cytokine production in RAW 264.7 macrophage cell exposed to human and commercial isolates of Bifidobacterium. Int. J. Food Microbiol. 46: 231-241 (1999) https://doi.org/10.1016/S0168-1605(98)00197-4
  21. Crittenden RG, Martinez NR, Playne MJ. Synthesis and utilization of folate by yoghurt starter cultures and probiotic bacteria. Int. J. Food Microbiol. 80: 217-222 (2003) https://doi.org/10.1016/S0168-1605(02)00170-8
  22. Samona A, Robinson RK, Marakis S. Acid production by bifidobacteria and yoghurt bacteria during fermentation and storage of milk. Food Microbiol. 13: 275-280 (1996) https://doi.org/10.1006/fmic.1996.0033
  23. Lotz M, Jirik F, Kabouridis P, Tsoukas C, Hirano T, Kishimoto T, Carson DA. B cell stimulating factor 2/interleukin 6 is a costimulant for human thymocytes and T lymphocytes. J. Exp. Med. 167: 1253-1258 (1988) https://doi.org/10.1084/jem.167.3.1253