Optimization for the Production of Mono- and Di-acylglycerols from Corn Oil by Enzymic Glycerolysis Using Response Surface Methodology

반응표면분석에 의한 옥수수유 유래 monoacylglycerol과 diacylglycerol 합성 조건의 최적화

  • Park, Rae-Kyun (Department of Food Science and Technology, Chungnam National University) ;
  • Choi, Sang-Won (Department of Food Science and Nutrition, Catholic University of Daegu) ;
  • Lee, Ki-Teak (Department of Food Science and Technology, Chungnam National University)
  • 박래균 (충남대학교 식품공학과) ;
  • 최상원 (대구가톨릭대학교 식품영양학과) ;
  • 이기택 (충남대학교 식품공학과)
  • Published : 2004.10.31

Abstract

Response surface methodology was used to optimize production conditions of monoacylglycerol (MAG) and diacylglycerols (DAG) from corn oil by enzymic glycerolysis. Contents of $1,3-DAG\;(Y_1),\;1,2-DAG\;(Y_2),\;total\;DAG\;(Y_3),\;MAG\;(Y_4)$, and total $DAG+MAG\;(Y_5)$ were obtained. Conditions were optimized using central composite design with incubation temperature $(35-75^{\circ}C,\;X_1)$, incubation time (1-11 hr, $X_2$), and amount of hexane added (0-2 mL, $X_3$) as three variables. Content of 1,3-DAG was maximized by 20.43 area% at incubation temperature of $44.92^{\circ}C$, incubation time of 10.24 hr, and hexane content of 1.16 mL, whereas that of 1,2-DAG (26.78 area%) was maximized at $56.32^{\circ}C$, 6.95 hr, and 1.04 mL, respectively. Predicted maximum total DAG content was 45.09 area% at $53.82^{\circ}C$, 8.03 hr, and 1.08 mL, while production conditions of MAG (9.57 arae%) were $64.14^{\circ}C$, 7.00 hr, and 0.13 mL. At variables of $54.07^{\circ}C$, 7.98 hr, and 1.02 mL, maximum content of total DAG+MAG predicted by RSM was 53.54 area%.

Keywords

RSM;diacylglycerol;monoacylglycerol;glycerolysis

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