Effect of Pretreatment Conditions on ${\gamma}-Aminobutyric$ Acid Content of Brown Rice and Germinated Brown Rice

전처리 조건이 현미 및 발아현미의 ${\gamma}-aminobutyric$ acid 함량에 미치는 영향

  • Published : 2004.10.31

Abstract

Effects of pretreatment conditions on ${\gamma}-aminobutyric$ acid (GABA) contents of brown rice and germinated brown rice were investigated. As steeping time increased, GABA contents of brown rices increased gradually. The highest GABA content, 3.33mg/100g, was found in brown rice steeped at $40^{\circ}C$ for 8 hr. GABA content of brown rice decreased significantly at pH 8 (p<0.05), but changed slightly at pH of steeping solution ranging 4-7. Steeping of brown rices in glutamate solution increased GABA contents. Brown rice steeped in glutamate solution at 200-300 ppm showed GABA content between $4.09{\pm}0.48-4.11{\pm}0,47mg/100g$, which was much higher than that of untreated brown rice. Anaerobic treatment of brown rices using $N_2$ gas increased GABA contents, ranging from $4,70{\pm}0.49\;to\;4.92{\pm}0.83mg/100g$. Germinated brown rice steeped in glutamate solution under anaerobic condition had GABA content of $5.92{\pm}0.72mg/100g$, two-fold higher than that of untreated brown rice, $3.05{\pm}0.67mg/100g$. Optimum pretreatment condition established in this study could significantly increase GABA content in germinated brown rice.

Keywords

germinated brown rice;${\gamma}-aminobutyric$ acid;pretreatment

References

  1. Elkhalil EAI, Tinay AH, Mohamed BE, Elsheikh EAE. Effect of malt pretreatment on phytic acid and in vitro protein digestibility of sorghum flour. Food Chem. 72: 29-32 (2001) https://doi.org/10.1016/S0308-8146(00)00195-3
  2. Mwikya SM, Camp JV, Yiru Y, Huyghebaert A. Nutrient and antinutrient changes in finger millet (Eleusine coracana) during sprouting. Lebensm. Wiss. Technol. 33: 9-14 (2000) https://doi.org/10.1006/fstl.1999.0605
  3. Okada T, Sugishita T, Murakami T, Murai H, Saikusa T, Horino T, Onoda A, Kajimoto O, Takahashi R, Takahashi T. Effect of the defatted rice germ enriched with GABA for sleeplessness, depression autonomic disorder by oral administration. J. Jpn. Soc. Food Sci. Technol. 47: 596-603 (2000) https://doi.org/10.3136/nskkk.47.596
  4. Boun AW, Shelp BJ. The metabolism and function of $\gamma$-aminobutyric acid. Plant Physiol. 115: 1-5 (1997) https://doi.org/10.1104/pp.115.1.1
  5. Saikusa T, Horino T, Mori Y. Distribution of free amino acids in the rice kernel and kernel fractions and the effect of water soaking on the distribution. J. Agic. Food Chem. 42: 1122-1125 (1994) https://doi.org/10.1021/jf00041a015
  6. Sawai Y, Konomi K, Odaka Y, Yoshitomi H, Yamaguchi Y, Miyama D. Contents of γ-aminobutyric acid in stem of anaerobic incubated tea shoot. Nippon Shokuhin Kagaku Kogaku Kaishi 46: 274-277 (1999) https://doi.org/10.3136/nskkk.46.274
  7. Sawai Y, Konomi K, Odaka Y, Yoshitomi H, Yamaguchi Y, Miyama D, Takeuchi A. Repeating treatment of anaerobic and aerobic incubation increases the amount of -aminobutyric acid in tea shoots. Nippon Shokuhin Kagaku Kogaku Kaishi 46: 462-466 (1999) https://doi.org/10.3136/nskkk.46.462
  8. Yun SJ, Choi KG, Kim JK. Effect of anaerobic treatment on carbohydrate- hydrolytic enzyme activities and free amino acid contents in barley malt. Korean J. Crop Sci. 43: 19-22 (1998)
  9. Ohisa N, Sugawara M, Abe Y, Kumagai M, Takahashi S. Accumulation of -$\gamma$aminobutyric acid by rice with germs and chicken soup. J. Jpn. Soc. Food Sci. Technol. 47: 452-454 (2000) https://doi.org/10.3136/nskkk.47.452
  10. Knight MR, Campbell AK, Smith SM, Trewavas HJ. Transgenic plant aequorin reports the effects of touch and cold shock and elicitors on cytoplasmic calcium. Nature 352: 524-526 (1991) https://doi.org/10.1038/352524a0
  11. Bergman EL, Autio K, Sandberg AS. Optimal conditions for phytate degradation, estimation of phytase activity and localization of phytate in barley(cv. Balenheim). J. Agric. Food Chem. 48: 4647-4655 (2000) https://doi.org/10.1021/jf000525a
  12. Sierra I, Vidal-Valverde C. Kinetics of free and glycosylated B6 vitamers, thiamin and riboflavin during germination of pea seeds. J. Sci. Food Agric. 73: 307-310 (1999)
  13. Wallace W, Secor J, Schrader LE. Rapid accumulation of $\gamma$-aminobutyric acid and alanine in soybean leaves in response to an abrupt transfer to lower temperature, darkness or mechanical manipulation. Plant Physiol. 75: 170-175 (1984) https://doi.org/10.1104/pp.75.1.170
  14. Saikusa T, Horino T, Mori Y. Accumulation of $\gamma$-aminobutyric acid (GABA) in the rice germ during water soaking. Biosci. Biotech. Biochem. 58: 2291-2292 (1994) https://doi.org/10.1271/bbb.58.2291
  15. Oh SH, Kim SH, Moon YJ, Choi WG. Changes in the levels of -$\gamma$aminobutyric acid and some amino acids by application of a glutamic acid solution for the germination of brown rices. Korean J. Biotechnol. Bioeng. 17: 49-53 (2002)
  16. Zhang G, Bown AW. The rapid determination of $\gamma$-aminobutyric acid. Phytochemistry 44: 1007-1009 (1997) https://doi.org/10.1016/S0031-9422(96)00626-7
  17. Oh SH, Lee IT, Park KB, Kim BJ. Changes in the levels of water soluble protein and free amino acids in brown rice germinated in a chitosan/glutamic acid solution. Korean J. Biotechnol. Bioeng. 17: 515-519 (2002)
  18. Kumar S, Punekar NS, SatyaNarayan V, Venkatesh KV. Metabolic fate of glutamate and evaluation of flux through the 4-aminobutyrate (GABA) shunt in Aspergillus niger. Biotechnol. Bioeng. 67: 575-584 (2000) https://doi.org/10.1002/(SICI)1097-0290(20000305)67:5<575::AID-BIT8>3.0.CO;2-L
  19. Oh SH, Choi WG. Production of the quality germinated brown rices containing high $\gamma$-aminobutyric acid by chitosan application. Korean J. Biotechnol. Bioeng. 15: 615-620 (2000)
  20. Streeter LG, Thompson JF. Anaerobic accumulation of $\gamma$-aminobutyric acid and alanine in radish leaves (Raphanus sativus L.). Plant Physiol. 49: 572-578 (1972) https://doi.org/10.1104/pp.49.4.572
  21. Yun SJ, Yoo NH. Changes of free amino acid and free sugar contents in barley seedlings in response to anaerobic or cold treatment. Korean J. Crop Sci. 41: 139-144 (1996)