Protection of Infection and Eradication Activity of Culture Product by Pediococcus pentosaceus CBT SL4 Showing Antimicrobial Activity against Helicobacter pylori

Helicobacter pylori에 대한 항균활성을 나타내는 Pediococcus pentosaceus CBT SL4 배양물의 감염방어 및 제균활성

  • Published : 2004.10.31


New food ingredient was developed to eradicate and protect against re-infection of Helicobacter pylori in fermentation broth of lactic acid bacteria (LAB) showing antimicrobial activity against pathogenic microorganisms such as H. pylori and Listeria monocytogenes. LAB strain CBT SL4 was identified as Pediococcus pentosaceus by 16S rDNA sequencing and its culture broth showed antimicrobial activity of 800 AU/mL against H. pylori in optimized fermentation process. Using thin layer concentration system and spray-typed fluid bed drier system, concentrated powder product showing activity of 12,800 AU/g was harvested. Product showed eradication and protection activities against H. pylori infection on feeding test (50 AU/day) using Mongolian gerbil infection model. After 4 weeks therapy of 8,000 AU/day, ${\Delta}13CO_2$ level (DOB30) decreased about 40% in urea breath test on patient with H. pylori infection. Result show concentrated culture product of P. pentosaceus CBT SL4 has eradicating and protecting activities against H. pylori infection and can be used as food-active ingredient for prevention of gastric and duodenum ulcer caused by H. pylori.


antimicrobial activity;eradication;fermentation;Helicobacter pylori;Pediococcus pentosaceus CBT SL4


  1. Yi JS, Kwon KS, Hong ES, Shin HJ, Park YJ, Cho HG, Kim PS, Lee DH, Choi W, Shin YW, Kim YS. Correlation between hyperammonemia and gastric Helicobacter pylori infection in patients with liver cirrhosis. J. Korean Gastroenterol. 34: 338-344 (1999)
  2. Shirota T. Helicobacter pylori infection and severity of reflux esopagitis. Jpn. Pharmacol. Ther. 25: 55-59 (1997)
  3. Kim BJ, Kang BH, Kim TY, Kim TH, Kim KW. Production and characterization of IgY specific to Helicobacter pylori. Korean J. Appl. Microbiol. Biotechnol. 25: 612-616 (1997)
  4. Koo JK, Kim CH, Choe TB. Inhibition of growth and adhesion of Helicobacter pylori using egg yolk antibodies. Biotechnol. Bioprocess Eng. 4: 219-223 (1999)
  5. Jung HC, Effect of fermented milk on the Helicobacter pylori infection in human stomach mucous. J. Korean Public Health Assoc. 27: 193-197 (2001)
  6. Jang BK, Ahn SH, Hu JW, Hwang JS, Kang YW, Park SK. The effect of Helicobacter pylori infection on peptic ulcer bleeding. J. Korean Gastroenterol. 34: 295-300 (1999)
  7. Won BR, Song EH, Kang GH, Chang MW. Yoon YH. Inhibitory effect of Lactobacillus helveticus CU 631 on urease and vacuolating toxin activity of Heliobacter pylori. J. Korean Anim. Sci. Technol. 43: 931-940 (2001)
  8. Servin AL. Antagonistic activity against Helicobacter pylori infection by the lactic acid bacteria. J. Korean Pubic Health Assoc. 27: 5-12 (2001)
  9. Dunn BE, Cohen H, Blaser MJ. Helicobacter pylori. J. Clin. Microbiol. Rev.10: 720-741 (1997)
  10. Ha KY. Recent trend of Helicobacter pylori infection. J. Korean Soc. Chemother. 16: 307-325 (1998)
  11. Roe IH, Yang MR, Kim JT, Joe JH, Lee JH. Ammonia induced gastric mucosal injury: Role of ammonia in the mechanism of Helicobacter pylori-related gastric disease. J. Korean Gastroenterol. 29: 442-448 (1997)
  12. Baik SC, Kim JB, Cho MJ, Kim YC, Park CK, Ryou HH, Choi HJ, Lee KH. Prevalence of Helicobacter pylori infection among normal Korean adults. J. Korean Soc. Microbiol. 25: 455-462 (1990)
  13. Ree KH, Youn HS, Baik SC, Lee WK, Cho MJ, Choi HJ, Maeng KY, Ko KW. Prevalence of Helicobacter pylori infection in Korea. J. Korean Soc. Microbiol. 25: 474-490 (1990)
  14. Miyagawa N. Effects of butyric acid bacteria on the loose stools of the Helicobacter pylori eradication. Jpn. Pharmacol. Ther. 27: 59-63 (1999)
  15. Koga Y. Helicobacter pylori association with gastric disease including gastric cancer. J. Korean Public Health Assoc. 27: 177- 180 (2001)
  16. Koo JK, Choe TB, Studies on adherance inhitition and detachment of Helicobacter pylori using egg yolk IgY and additives. Korean J. Biotechnol. Bioeng. 16: 41-47 (2001)
  17. Lee YH, Shin EJ, Lee JH, Park JH, Lactobacillus acidophilus inhibits the Helicobacter pylori adherence. J. Microbiol. Biotechnol. 9: 794-797 (1999)