Screening of Bifidobacterium spp. for the Development of Infant Probiotics

유아용 생균제 개발을 위한 Bifidobacterium spp.의 선발

  • Published : 2004.10.31


Bifidobacterium spp. exhibits the highest number of counts among species of microflora in breast-feeding infant intestines and has been used as probiotics. From infant groups with different diets, 42 Bifidobacterial strains were isolated by selective plate, Gram-staining, and morphology using method of Mitsuoka, among which seven isolates were identified as Bifidobacterium spp. by F6PPK test, MIDI, and PCR. B. bifidum PBH-30, selected for development of probiotics, showed high resistance against low pH and oxgall treatment, and inhibition against pathogens such as Salmonella typhimurium and Staphylococcus aureus. B. bifidum PBH-30 could be applicable to dairy products as probiotic strains due to its excellent growth in raw milk.




  1. Hilton E, Henry DI, Phyllis A, Kenneth F, Michael TB. Ingestion of yoghurt containing Lactobacillus acidophilus as prophylaxis for candidal vaginitis. Ann. Int. Med. 116:353-357 (1992)
  2. Gillialand SE, Walker DK. Factors to consider when selecting a culture of Lactobacillus acidophilus as a dietary adjunct to produce a hypocholesterolemic effect in humans. J. Dairy Sci. 73: 905-911 (1990)
  3. Shin MS, Lee JF, Na SH, Bae GS, Huh CS, Baek YJ. Characteristics of Bifidobacterium spp. isolated from Korean feces for probiotics. Korean J. Food Sci. Technol. 31: 495-501 (1999)
  4. Kirjavainen PV, Apostolou E, Arvola T, Salminen SJ, Gibson GR, Isolauri E. Characterizing the composition of intestinal microflora as a prospective treatment target in infant allergic disease. FEMS Immunol. Med. Microbiol. 32: 1-7 (2001)
  5. Salminen S, Bouley MC, Boutron-Rualt MC, Cummings J, Franck A, Gibson G, Isolauri E, Moreau MC, Roberfroid M, Rowland I. Functional food science and gastrointestinal physiology and function. Br. J. Nutr. Suppl. 1: 147-171 (1998)
  6. Matszaki T, Hashimoto S, Yokokura T. Effects on antitumor activity and cytokine production in the thoracic cavity by intrapleural administration of Lactobacillus casei in tumor-bearing mice. Med. Microbiol. Immunol. Berl. 185: 157-161 (1996)
  7. Mitsuoka T. The World of Anaerobic Bacteria: A Color Atlas of Anaerobic Bacteria. Sobun Press, Tokyo, Japan. pp. 13-65 (1980)
  8. Takiguchi R, Mochizuki E, Suzuki Y, Nakajima I, Benno Y. Lactobacillus acidophilus SBT2062 on harmful intestinal bacteria. J. Int. Microbiol. 11: 11-17 (1997)
  9. Goldin BR, Borbach SD. Alterations in fecal microflora enzymes related to diet, age, lactobacillus supplements, and dimethylhydrazine. Cancer 40: 2421-2426 (1977)
  10. Salminen S, Salminen E. Lactulose, lactic acid bacteria, intestinal microecology, and mucosal protection. Scand. J. Gastroenterol. Suppl. 222: 45-48 (1997)
  11. Fuller R. Probiotics in man and animals. J. Appl. Bacteriol. 66: 365-378 (1989)
  12. Mitsuoka T. Bifidobacteria and their role in human health. J. Ind. Microbiol. 6: 263-268 (1990)
  13. Shah N. Lactobacillus acidophilus and lactose intolerance: A review. Asean Food J. 9: 47-54 (1994)
  14. Clark PA, Cotton LN, Martin JH. Selection of bifidobateria for use as dietary adjuncts in cultured dairy foods: II-Tolerance to simulated pH of human stomachs. Cult. J. Dairy Prod. 28: 11-14 (1993)
  15. Scardovi V. The genus Bifidobacterium. In: Bergey's Manual of Systematic Bacteriology. Holt JG (ed). Williams and Wikins Co., Baltimore, MD, USA (1986)
  16. Kok RG, Waal A-de, Schut F, Welling GW, Weenk G, Hellingwerf KJ. Specific detection and analysis of a probiotic Bifidobacterium strain in infant feces. Appl. Environ. Microbiol. 62: 3668-3672 (1996)