Screening of Bifidobacterium spp. for the Development of Infant Probiotics

유아용 생균제 개발을 위한 Bifidobacterium spp.의 선발

  • Yang, Hyun-Ju (Department of Food Science and Technology and the Research Center for Bioresource and Health, Chungbuk University) ;
  • Jang, Keum-Il (Department of Food Science and Technology and the Research Center for Bioresource and Health, Chungbuk University) ;
  • Kim, Chung-Ho (Department of Food and Nutrition, Seowon University) ;
  • Lee, Yoon-Bok (Central Research Institute, Dr. Chung's Food Company, Ltd.) ;
  • Sohn, Heon-Soo (Central Research Institute, Dr. Chung's Food Company, Ltd.) ;
  • Kim, Kwang-Yup (Department of Food Science and Technology and the Research Center for Bioresource and Health, Chungbuk University)
  • 양현주 (충북대학교 식품공학과 및 생물건강산업개발연구센터) ;
  • 장금일 (충북대학교 식품공학과 및 생물건강산업개발연구센터) ;
  • 김정호 (서원대학교 식품영양학과) ;
  • 이윤복 ((주)정식품 중앙연구소) ;
  • 손헌수 ((주)정식품 중앙연구소) ;
  • 김광엽 (충북대학교 식품공학과 및 생물건강산업개발연구센터)
  • Published : 2004.10.31


Bifidobacterium spp. exhibits the highest number of counts among species of microflora in breast-feeding infant intestines and has been used as probiotics. From infant groups with different diets, 42 Bifidobacterial strains were isolated by selective plate, Gram-staining, and morphology using method of Mitsuoka, among which seven isolates were identified as Bifidobacterium spp. by F6PPK test, MIDI, and PCR. B. bifidum PBH-30, selected for development of probiotics, showed high resistance against low pH and oxgall treatment, and inhibition against pathogens such as Salmonella typhimurium and Staphylococcus aureus. B. bifidum PBH-30 could be applicable to dairy products as probiotic strains due to its excellent growth in raw milk.


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