Screening of Bifidobacterium spp. for the Development of Infant Probiotics

유아용 생균제 개발을 위한 Bifidobacterium spp.의 선발

  • Published : 2004.10.31

Abstract

Bifidobacterium spp. exhibits the highest number of counts among species of microflora in breast-feeding infant intestines and has been used as probiotics. From infant groups with different diets, 42 Bifidobacterial strains were isolated by selective plate, Gram-staining, and morphology using method of Mitsuoka, among which seven isolates were identified as Bifidobacterium spp. by F6PPK test, MIDI, and PCR. B. bifidum PBH-30, selected for development of probiotics, showed high resistance against low pH and oxgall treatment, and inhibition against pathogens such as Salmonella typhimurium and Staphylococcus aureus. B. bifidum PBH-30 could be applicable to dairy products as probiotic strains due to its excellent growth in raw milk.

Keywords

Bifidobacterium;infant;screening;microflora;probiotics

References

  1. Hilton E, Henry DI, Phyllis A, Kenneth F, Michael TB. Ingestion of yoghurt containing Lactobacillus acidophilus as prophylaxis for candidal vaginitis. Ann. Int. Med. 116:353-357 (1992) https://doi.org/10.7326/0003-4819-116-5-353
  2. Gillialand SE, Walker DK. Factors to consider when selecting a culture of Lactobacillus acidophilus as a dietary adjunct to produce a hypocholesterolemic effect in humans. J. Dairy Sci. 73: 905-911 (1990) https://doi.org/10.3168/jds.S0022-0302(90)78747-4
  3. Shin MS, Lee JF, Na SH, Bae GS, Huh CS, Baek YJ. Characteristics of Bifidobacterium spp. isolated from Korean feces for probiotics. Korean J. Food Sci. Technol. 31: 495-501 (1999)
  4. Kirjavainen PV, Apostolou E, Arvola T, Salminen SJ, Gibson GR, Isolauri E. Characterizing the composition of intestinal microflora as a prospective treatment target in infant allergic disease. FEMS Immunol. Med. Microbiol. 32: 1-7 (2001) https://doi.org/10.1111/j.1574-695X.2001.tb00526.x
  5. Salminen S, Bouley MC, Boutron-Rualt MC, Cummings J, Franck A, Gibson G, Isolauri E, Moreau MC, Roberfroid M, Rowland I. Functional food science and gastrointestinal physiology and function. Br. J. Nutr. Suppl. 1: 147-171 (1998)
  6. Matszaki T, Hashimoto S, Yokokura T. Effects on antitumor activity and cytokine production in the thoracic cavity by intrapleural administration of Lactobacillus casei in tumor-bearing mice. Med. Microbiol. Immunol. Berl. 185: 157-161 (1996) https://doi.org/10.1007/s004300050026
  7. Mitsuoka T. The World of Anaerobic Bacteria: A Color Atlas of Anaerobic Bacteria. Sobun Press, Tokyo, Japan. pp. 13-65 (1980)
  8. Takiguchi R, Mochizuki E, Suzuki Y, Nakajima I, Benno Y. Lactobacillus acidophilus SBT2062 on harmful intestinal bacteria. J. Int. Microbiol. 11: 11-17 (1997)
  9. Goldin BR, Borbach SD. Alterations in fecal microflora enzymes related to diet, age, lactobacillus supplements, and dimethylhydrazine. Cancer 40: 2421-2426 (1977)
  10. Salminen S, Salminen E. Lactulose, lactic acid bacteria, intestinal microecology, and mucosal protection. Scand. J. Gastroenterol. Suppl. 222: 45-48 (1997)
  11. Fuller R. Probiotics in man and animals. J. Appl. Bacteriol. 66: 365-378 (1989) https://doi.org/10.1111/j.1365-2672.1989.tb05105.x
  12. Mitsuoka T. Bifidobacteria and their role in human health. J. Ind. Microbiol. 6: 263-268 (1990) https://doi.org/10.1007/BF01575871
  13. Shah N. Lactobacillus acidophilus and lactose intolerance: A review. Asean Food J. 9: 47-54 (1994)
  14. Clark PA, Cotton LN, Martin JH. Selection of bifidobateria for use as dietary adjuncts in cultured dairy foods: II-Tolerance to simulated pH of human stomachs. Cult. J. Dairy Prod. 28: 11-14 (1993)
  15. Scardovi V. The genus Bifidobacterium. In: Bergey's Manual of Systematic Bacteriology. Holt JG (ed). Williams and Wikins Co., Baltimore, MD, USA (1986)
  16. Kok RG, Waal A-de, Schut F, Welling GW, Weenk G, Hellingwerf KJ. Specific detection and analysis of a probiotic Bifidobacterium strain in infant feces. Appl. Environ. Microbiol. 62: 3668-3672 (1996)