Quantification of Genetically Modified Soy Proteins in Fresh Soybean Curd by Antigen-coated Plate ELISA

효소면역측정법을 이용한 두부 중의 유전자 재조합 대두단백질 분석

  • Jung, Mee-Hyun (Department of Applied Biology and Chemistry, KonKuk University) ;
  • Bae, Hyung-Ki (Department of Applied Biology and Chemistry, KonKuk University) ;
  • Kim, Kyung-Mi (Department of Applied Biology and Chemistry, KonKuk University) ;
  • Jang, In-Suk (Department of Applied Biology and Chemistry, KonKuk University) ;
  • Ko, Eun-Jung (Department of Applied Biology and Chemistry, KonKuk University) ;
  • Bae, Dong-Ho (Department of Applied Biology and Chemistry, KonKuk University)
  • 정미현 (건국대학교 응용생물화학과) ;
  • 배형기 (건국대학교 응용생물화학과) ;
  • 김경미 (건국대학교 응용생물화학과) ;
  • 장인숙 (건국대학교 응용생물화학과) ;
  • 고은정 (건국대학교 응용생물화학과) ;
  • 배동호 (건국대학교 응용생물화학과)
  • Published : 2004.10.31


Enzyme-linked immune sorbent assay (ELISA) was applied to quantify soy protein in fresh soybean curd (bean curd) produced by combination of genetically modified (GM) and genetically not modified (non-GM) soybeans. Antibodies against 113 and 24 kDa proteins, which appeared only in non-GM bean curd (specific band), and in both non-GM and GM bean curds (non-specific band) based on SDS-PAGE results, were prepared by immunization to rabbit. Through ELISA using either antibody, GM bean curd protein content was determined at dilution rates of $10^{-1}-10^{-6}$. Standard curve showing relationship between ELISA optical density and non-GM protein content was produced using antibody against 113 kDa protein at protein dilution between $10^{-7}\;to\;10^{-6}$, highly antigen content-dependent dilution. Bean curd prepared by random combinations of GM and non-GM soybeans were analyzed by ELISA, and standard curve was produced. Results reveal non-GM protein content of bean curd could be quantified with higher than 93% accuracy.


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