Anticancer Effect of Erythronium japonicum Extract on ICR Mouse and L1210 Cells with Alteration of Antioxidant Enzyme Activities

얼레지 추출물의 ICR 마우스와 L1210 암세포에 대한 항암작용과 그에 따른 항산화효소 활성변화

  • Shin, Yoo-Jin (Department of Chemistry, Sangmyung University) ;
  • Jung, Dae-Young (Department of Herbal Pharmaceutical Medicine, Korea Institute of Oriental Medicine) ;
  • Ha, Hye-Kyung (Department of Chemistry, Sangmyung University) ;
  • Park, Sie-Won (Department of Chemistry, Sangmyung University)
  • 신유진 (상명대학교 화학과) ;
  • 정대영 (한국 한의학 연구원 한약제재 개발부) ;
  • 하혜경 (상명대학교 화학과) ;
  • 박시원 (상명대학교 화학과)
  • Published : 2004.12.31


Effects of Erythronium japonicum methanol extract on ICR mouse with induced abdominal cancer and L1210 cells were studied. Administration of methanol extract ($10-100\;{\mu}g/20\;g$ body weight) prolonged life by 47.8% and decreased number of L1210 cells with $IC_{50}\;of\;54.6\;{\mu}g/mL$ after 3 days culture, whereas little effect was observed against normal lymphocytes (<6% compared to 83.2% of L1210 cells under the same condition). Increased SOD and GPx enzyme activities, and remarkably augmented generation of ${O_2}^-$ ion in L1210 cells by E. japonicum extract, implied that reactive oxygen species including ${O_2}^-$ ion, might have participated in L1210 cell death


Erythronium japonicum;anticancer activity;ICR mouse;L1210 cells;superoxide dismutase;glutathione peroxidase


  1. Serrano J, Palmeria CM, Kuehl DW. Wallace KB. Cardioselctive and cumulative oxidation of mitochondrial DNA following subchromic doxoruicin administration. Biochem. Biophys. Acta 1411: 201-205 (1999)
  2. Boyum A. Isolation of leukocytes from human blood. Scan. J. Clin. Invest. 21: 9-15 (1968)
  3. Thayer PS, Himmelfarb P, Watts GI. Cytotoxicity assays with L1210 cells in vitro: Comparison with L1210 in vivo and KB cells in vitro. Cancer Chemother. Rep. (part 2) 2: 1-25 (1971)
  4. Maral J, Puget K, Michelson AM. Comparative study of superoxide dismutase, catalase, glutathione peroxidase levels in erythrocytes of different animals. Biochem. Biophys. Res. Comm. 77: 1525-1531 (1997)
  5. Astrow AB. Rethinking cancer. Lancet 343: 494-503 (1994)
  6. Amstad P, Moret R, Cerutti P. Glutathione peroxidase compensates for the hypersensativity of Cu-Zn superoxide dismutase overproducers to oxidant stress. J. Biol. Chem. 58: 1606-1612 (1994)
  7. Cooper PD, Carter M. The anti-melanoma activity of inulin in mice. Mol. Immunol. 23: 903-911 (1986)
  8. Orrenius S. Mechanisms of Oxidative Cell Damage: An Overview of Oxidative Process and Antioxidants. Raven Press Ltd., New York, NY, USA. pp.53-71 (1994)
  9. Bailer JC, Gormick HL. Cancer undefeated. N. Eng. J. Med. 336: 1569-1575 (1997)
  10. McCord J, Fridovich I. Superoxide dismutase. An enzymatic function for erythrocuprein (heterocuprein). J. Biol. Chem. 244: 6049-6052 (1969)
  11. Evans MD, Griffith HR, Lunec J. Reactive oxygen species and their cytotoxic mechanisms. Adv Mol. Cell. Biol. 20: 25-31 (1997)
  12. Sould AK, Tacka KA, Galvan KA, Penefsky HS. Immediate effects of anticancer drugs on mitochondrial oxygen consumption. Biochem. Pharmacol. 66: 977-987 (2003)
  13. Ritsena T, Smeekens M. Fructans: beneficial for plants and humans. Curr. Opin. Plant Biol. 6: 223-229 (2003)
  14. Reiter RJ. Oxidative processes and antioxidative defense mechanism in the aging brain. FASEB J. 9: 528-534 (1995)
  15. Wiseman H, Halliwell B. Damage to DNA by reactive oxygen species and nitrogen species in inflammatory disease and progression to cancer. Biochem. J. 313: 1729-1734 (1996)
  16. Markesbery WR. Oxidative stress hypothesis in Alzheimer disease. Free Rad. Biol. Med. 23: 134-139 (1994)
  17. Mullin WJ, Peacock S, Loewen DC, Turner NJ. Macronutrients content of yellow glacierlily and balsamroot; root vegetables used by indigenous peoples of north western north America. Food Res. Intl. 30: 769-775 (1997)
  18. Steel VE. Current mechanistic approaches to the chemoprevention of cancer. J. Biochem. Mol. Biol. 36: 78-81 (2003)
  19. Reynolds CP, Maureer BJ. Kolesnick RN. Ceramide synthesis and metabolismas as target for cancer therapy. Cancer Lett. 206: 169 -180 (2004)