Anticancer Effect of Erythronium japonicum Extract on ICR Mouse and L1210 Cells with Alteration of Antioxidant Enzyme Activities

얼레지 추출물의 ICR 마우스와 L1210 암세포에 대한 항암작용과 그에 따른 항산화효소 활성변화

  • Shin, Yoo-Jin (Department of Chemistry, Sangmyung University) ;
  • Jung, Dae-Young (Department of Herbal Pharmaceutical Medicine, Korea Institute of Oriental Medicine) ;
  • Ha, Hye-Kyung (Department of Chemistry, Sangmyung University) ;
  • Park, Sie-Won (Department of Chemistry, Sangmyung University)
  • 신유진 (상명대학교 화학과) ;
  • 정대영 (한국 한의학 연구원 한약제재 개발부) ;
  • 하혜경 (상명대학교 화학과) ;
  • 박시원 (상명대학교 화학과)
  • Published : 2004.12.31


Effects of Erythronium japonicum methanol extract on ICR mouse with induced abdominal cancer and L1210 cells were studied. Administration of methanol extract ($10-100\;{\mu}g/20\;g$ body weight) prolonged life by 47.8% and decreased number of L1210 cells with $IC_{50}\;of\;54.6\;{\mu}g/mL$ after 3 days culture, whereas little effect was observed against normal lymphocytes (<6% compared to 83.2% of L1210 cells under the same condition). Increased SOD and GPx enzyme activities, and remarkably augmented generation of ${O_2}^-$ ion in L1210 cells by E. japonicum extract, implied that reactive oxygen species including ${O_2}^-$ ion, might have participated in L1210 cell death


  1. Serrano J, Palmeria CM, Kuehl DW. Wallace KB. Cardioselctive and cumulative oxidation of mitochondrial DNA following subchromic doxoruicin administration. Biochem. Biophys. Acta 1411: 201-205 (1999)
  2. Boyum A. Isolation of leukocytes from human blood. Scan. J. Clin. Invest. 21: 9-15 (1968)
  3. Thayer PS, Himmelfarb P, Watts GI. Cytotoxicity assays with L1210 cells in vitro: Comparison with L1210 in vivo and KB cells in vitro. Cancer Chemother. Rep. (part 2) 2: 1-25 (1971)
  4. Maral J, Puget K, Michelson AM. Comparative study of superoxide dismutase, catalase, glutathione peroxidase levels in erythrocytes of different animals. Biochem. Biophys. Res. Comm. 77: 1525-1531 (1997)
  5. Astrow AB. Rethinking cancer. Lancet 343: 494-503 (1994)
  6. Amstad P, Moret R, Cerutti P. Glutathione peroxidase compensates for the hypersensativity of Cu-Zn superoxide dismutase overproducers to oxidant stress. J. Biol. Chem. 58: 1606-1612 (1994)
  7. Cooper PD, Carter M. The anti-melanoma activity of inulin in mice. Mol. Immunol. 23: 903-911 (1986)
  8. Orrenius S. Mechanisms of Oxidative Cell Damage: An Overview of Oxidative Process and Antioxidants. Raven Press Ltd., New York, NY, USA. pp.53-71 (1994)
  9. Bailer JC, Gormick HL. Cancer undefeated. N. Eng. J. Med. 336: 1569-1575 (1997)
  10. McCord J, Fridovich I. Superoxide dismutase. An enzymatic function for erythrocuprein (heterocuprein). J. Biol. Chem. 244: 6049-6052 (1969)
  11. Evans MD, Griffith HR, Lunec J. Reactive oxygen species and their cytotoxic mechanisms. Adv Mol. Cell. Biol. 20: 25-31 (1997)
  12. Sould AK, Tacka KA, Galvan KA, Penefsky HS. Immediate effects of anticancer drugs on mitochondrial oxygen consumption. Biochem. Pharmacol. 66: 977-987 (2003)
  13. Ritsena T, Smeekens M. Fructans: beneficial for plants and humans. Curr. Opin. Plant Biol. 6: 223-229 (2003)
  14. Reiter RJ. Oxidative processes and antioxidative defense mechanism in the aging brain. FASEB J. 9: 528-534 (1995)
  15. Wiseman H, Halliwell B. Damage to DNA by reactive oxygen species and nitrogen species in inflammatory disease and progression to cancer. Biochem. J. 313: 1729-1734 (1996)
  16. Markesbery WR. Oxidative stress hypothesis in Alzheimer disease. Free Rad. Biol. Med. 23: 134-139 (1994)
  17. Mullin WJ, Peacock S, Loewen DC, Turner NJ. Macronutrients content of yellow glacierlily and balsamroot; root vegetables used by indigenous peoples of north western north America. Food Res. Intl. 30: 769-775 (1997)
  18. Steel VE. Current mechanistic approaches to the chemoprevention of cancer. J. Biochem. Mol. Biol. 36: 78-81 (2003)
  19. Reynolds CP, Maureer BJ. Kolesnick RN. Ceramide synthesis and metabolismas as target for cancer therapy. Cancer Lett. 206: 169 -180 (2004)