Effect of Intestinal Function Enhancer (KTG075) on Mucin 2 Secretion

장기능개선제-신소재(KTG075)의 대장관 내 mucin 2 분비에 미치는 영향

  • Published : 2004.12.31

Abstract

Effects of formulation KTG075 from edible plants on intestinal function, particularly on Mucin 2 secretion, were examined by loperamide-induced constipation method using Sprague Dawley rats (SD rats, male). Crypt epithelial cells containing more mucus and mucus layer stained with alcian blue were significantly thicker in KTG075 group than control group. When Biogenex AM358 of antibody against Mucin 2 was used, crypt epithelial cells secreted more Mucin 2 in KTG075 group than control group. The Mucus layer at fecal surface was thinner and less mucus was recovered from mucosal surface in constipated rats than in KTG075 group. Mucus production of crypt epithelial cells and mucus contents at fecal and mucosal surfaces were reduced by loperamide-induced constipation. These results indicate formula KTG075 accelerates evacuation and activates intestines.

Keywords

intestinal function;improving agent constipation;loperamide-induced constipation method;charcoal meal transit;KTG075;mucus layer;mucin;mucin 2

References

  1. Loeschke K, Schmid T, Farack UM. Inhibition by loperamide of mucus secretion in the rat colon in vivo. Eur. J. Pharmacol. 170: 41-46 (1989) https://doi.org/10.1016/0014-2999(89)90131-3
  2. Shimotoyodome A, Meguro S, Hase T, Tokimitsu I, Sakata T. Decreased colonic mucus in rats with loperamide-induced constipation. Comp. Biochem. Physiol. Part A 126: 203-211 (2000) https://doi.org/10.1016/S1095-6433(00)00194-X
  3. Sakata T, Engelhardt WV. Luminal mucin in the large intestine of mice, rats and guinea pigs. Cell. Tissue. Res. 219: 629-635 (1981)
  4. Derker J, John WA, Rossen HA. The MUC family: an obituary. Trends Biochem. Sci. 27(3): 126-131 (2002) https://doi.org/10.1016/S0968-0004(01)02052-7
  5. Griffiths B. Assignment of the polymorphic intestinal mucin gene(MUC2) to chromosome. Ann. Hum. Genet. 4: 277-285 (1990)
  6. Kristien MAJ, Tytgat JWG, Alexandra WCE, Hans AB, Jan D. Quantitative analysis of MUC2 synthesis in ulcerative colitis. Biochem. Biophys. Res. Comm. 224: 397-405 (1996) https://doi.org/10.1006/bbrc.1996.1039
  7. Ouwehand AC, Kirjavainen PV. Adhension of probiotic microorganisms to intestinal mucus. Int. Dairy J. 9: 623-630 (1999) https://doi.org/10.1016/S0958-6946(99)00132-6
  8. Jass JR, Walsh MD. Altered mucin expression in the gastrointestinal tract: a review. J. Cell. Mol. Med. 5: 327-351 (2001) https://doi.org/10.1111/j.1582-4934.2001.tb00169.x
  9. Corfield AP, Carrol D, Myerscough N, Probert CSJ. Mucins in the gastrointestinal tract in health and disease. Front Biosci. 6: 1321-1327 (2001) https://doi.org/10.2741/Corfield
  10. Kirjavainen PV, Ouwenhand AC, Isolauri E, Salminen SJ. The ability of probiotic bacteria to bind to human intestinal bacteria. FEMS Microbiol. Lett. 167: 185-189 (1993) https://doi.org/10.1111/j.1574-6968.1998.tb13226.x
  11. Montagne L, Pluske JR, Hampson DJ. A review of interactions between dietary fiber and the intestinal mucosa, and their consequences on digestive health in young non-ruminant animals. Anim. Feed Sci. Technol. 108: 95-117 (2003) https://doi.org/10.1016/S0377-8401(03)00163-9
  12. Silberberg A, Meyer FA. Structure and funtion of mucus. Adv. Exp. Med. Biol. 144: 53-74 (1982)
  13. Sheehan JK, Carlstedt I. Models for the macromolecular structure of mucus glycoproteins. Spec. Publ. Soc. Chem. 74: 256-275 (1989)
  14. Tytgat KMAJ, Dekker J, Buller HA. Mucins in inflammatory bowel disease. Eur. J. Gastroenterol. Hepatol. 5: 119-128 (1993) https://doi.org/10.1097/00042737-199303000-00001
  15. Strous GJ, Dekker J. Mucin-type glycoproteins. Crit. Rev. Biochem. Mol. Biol. 27: 57-92(1992) https://doi.org/10.3109/10409239209082559