Purification and Characterization of Anticarcinogenic Compound from Corni fructus

산수유에 함유된 항암물질의 정제 및 특성

  • Kim, Byeong-Hyeon (Department of Oriental Medicine Resources, Sunchon National University) ;
  • Park, Kyung-Wuk (Department of Food and Nutrition, Sunchon National University) ;
  • Kim, Jae-Yong (Research Institute of Basic Science, Sunchon National University) ;
  • Jeong, Ill-Yun (Radiation Food Technology and Bioscience Team, Korea Atomic Energy Research Institute) ;
  • Yang, Gi-Ho (Department of Oriental Medicine Resources, Sunchon National University) ;
  • Cho, Young-Sook (Department of Food and Nutrition, Sunchon National University) ;
  • Yee, Sung-Tae (Department of Biology, Sunchon National University) ;
  • Seo, Kwon-Il (Department of Food and Nutrition, Sunchon National University)
  • 김병현 (순천대학교 한약자원학과) ;
  • 박경욱 (순천대학교 식품영양학과) ;
  • 김재용 (순천대학교 기초과학연구소) ;
  • 정일윤 (한국원자력연구소 방사선식품.생명공학기술 개발팀) ;
  • 양기호 (순천대학교 한약자원학과) ;
  • 조영숙 (순천대학교 식품영양학과) ;
  • 이성태 (순천대학교 생물학과) ;
  • 서권일 (순천대학교 식품영양학과)
  • Published : 2004.12.31

Abstract

Chloroform layer from methanol extract of Corni fructus (Cornaceae) showed strong antiproliferation effect on human cancer cell lines by SRB assay. Anticarcinogenic-active compound was isolated and purified by silica gel column and thin layer chromatograpies, and identified as ursolic acid ($3{\beta}$-hydroxyrus-12-ene-28-oic acid, MW:456) by mass and IR spectrophotometries, and $^1H-and\;^{13}C-NMRs$. The compound inhibited proliferation of A549 (human lung cancer cell line) and MCF-7 (human breast cancer cell line) cells in dose-dependant manner when treated for 48 hr. Inhibition rates of both cells were over 40% and 90% compared with control cells at the $30\;{\mu}g/mL\;and\;100\;{\mu}g/mL$, respectively. Morphology of cells treated with the compound for 15 hr at $10\;{\mu}g/mL$ was distorted with shrinked cell mass, and cell number was lower than that of control cells. Cell cycle analysis showed sub-G1 phase arrest in both cell lines following 15 hr exposure to the compound; % of cell phase increased to 11.7 and 11.2% compared to the control of 4.0% and 2.1% in A549 and MCP-7 cells, respectively.

Keywords

Corni fructus;ursolic acid;cytotoxic activity;apoptosis

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