Purification and Characterization of Anticarcinogenic Compound from Corni fructus

산수유에 함유된 항암물질의 정제 및 특성

  • Kim, Byeong-Hyeon (Department of Oriental Medicine Resources, Sunchon National University) ;
  • Park, Kyung-Wuk (Department of Food and Nutrition, Sunchon National University) ;
  • Kim, Jae-Yong (Research Institute of Basic Science, Sunchon National University) ;
  • Jeong, Ill-Yun (Radiation Food Technology and Bioscience Team, Korea Atomic Energy Research Institute) ;
  • Yang, Gi-Ho (Department of Oriental Medicine Resources, Sunchon National University) ;
  • Cho, Young-Sook (Department of Food and Nutrition, Sunchon National University) ;
  • Yee, Sung-Tae (Department of Biology, Sunchon National University) ;
  • Seo, Kwon-Il (Department of Food and Nutrition, Sunchon National University)
  • 김병현 (순천대학교 한약자원학과) ;
  • 박경욱 (순천대학교 식품영양학과) ;
  • 김재용 (순천대학교 기초과학연구소) ;
  • 정일윤 (한국원자력연구소 방사선식품.생명공학기술 개발팀) ;
  • 양기호 (순천대학교 한약자원학과) ;
  • 조영숙 (순천대학교 식품영양학과) ;
  • 이성태 (순천대학교 생물학과) ;
  • 서권일 (순천대학교 식품영양학과)
  • Published : 2004.12.31


Chloroform layer from methanol extract of Corni fructus (Cornaceae) showed strong antiproliferation effect on human cancer cell lines by SRB assay. Anticarcinogenic-active compound was isolated and purified by silica gel column and thin layer chromatograpies, and identified as ursolic acid ($3{\beta}$-hydroxyrus-12-ene-28-oic acid, MW:456) by mass and IR spectrophotometries, and $^1H-and\;^{13}C-NMRs$. The compound inhibited proliferation of A549 (human lung cancer cell line) and MCF-7 (human breast cancer cell line) cells in dose-dependant manner when treated for 48 hr. Inhibition rates of both cells were over 40% and 90% compared with control cells at the $30\;{\mu}g/mL\;and\;100\;{\mu}g/mL$, respectively. Morphology of cells treated with the compound for 15 hr at $10\;{\mu}g/mL$ was distorted with shrinked cell mass, and cell number was lower than that of control cells. Cell cycle analysis showed sub-G1 phase arrest in both cell lines following 15 hr exposure to the compound; % of cell phase increased to 11.7 and 11.2% compared to the control of 4.0% and 2.1% in A549 and MCP-7 cells, respectively.


Corni fructus;ursolic acid;cytotoxic activity;apoptosis


  1. Dai Y, Hang B, Huang Z. Inhibition of Corni fructus on experimental inflammation. Chung Kuo Chung Yao Ts Chih 17: 307- 309 (1992)
  2. Won DH, Cho JH, Kim HS, Ko JH, Lee J, Park SA, Lee HJ, Yook CS, Kim IH, Won BP. Studies on the analysis of Corni fructus and its preparation. The Annual Report KFDA 1: 197-201 (1996)
  3. Rubinstein LV, Shoemaker RH, Paull KD, Simmon RM, Tosini S, Skehan P, Scudiero DA, Monks A, Boyd MR. Comparison of in vitro anticancer-drug-screening data generated with a tetrazdium assay versus a protein assay against a diverse panel of human tumer cell lines. J. Natl. Cancer Inst. 4: 1113-1118 (1990)
  4. Skehan P, Storeng S, Scudiero D, Monks A, McMahon J, Vistica D, Warren JT, Bokesch H, Keney S, Boyd MR. New colorimetric cytotoxicity assay for anticancer-drug screening. J. Nat. Cancer Inst. 82: 1107-1112 (1990) https://doi.org/10.1093/jnci/82.13.1107
  5. Murakami H, Sugahara T. Animal cell technology. Corona Publishing Co. Japan. pp. 215-233 (1994)
  6. Lee CK, Park SW, Chung HY, Young HS, Suh SS, Park KY. Mechanism of antitumor effect of ursolic acid from Eriobotrya Japonica. Korean J. Cancer Assoc. 23: 206-210 (1991)
  7. Yang A, Phillipson JD, Bowery NG. Four new triterpene glycosides from Schfflera bodinieri roots. J. Nat. Prod. 59: 1043-1046 (1996) https://doi.org/10.1021/np960235h
  8. Hah JC, Rhew TH, Choe ES, Chang HY, Park KY. Antitumor effect of ursolic acid against inbred hepatoma in CBA/J mouse. Korean J. Cancer Assoc. 24: 790-794 (1992)
  9. Park YK, Whang WK, Kim HI. The antidiabetic effects of extract from Cornus officinalis seed. Chung-Ang J. Pharm. Sci. 9: 5-11 (1995)
  10. Hwang YK, Kim DC, Hwang WI, Han YB. Inhibitory effect of artemisia princeps pampan. extaract on growth of cancer cell lines. Korean J. Nutr. 13: 799-808 (1998)
  11. Tsutomu N, Keishi T, Hiroko M, Midori S, Akira I. Phytochemical studies of seeds of medicinal plants. IV.: Ursolic acid and oleanolic acid glycosides from seeds. Patrinia Scabiosaefolia Fischer 41: 183-186 (1993)
  12. Guilian T, Zhang T, Yang F, Ito Y. Separation of galllic acid from Cornus officinalis Sieb. et Zucc by high-speed counter-current chromatography. J. Chromatogr. A. 886: 309-312 (2000) https://doi.org/10.1016/S0021-9673(00)00480-5
  13. Kim YH. Isolation of constituents from the fruits of Cornus officinalis. Siebold. 14: 287-292 (1999)
  14. Lee JY. Iridoid glycosides of Cornus officinalis. MS thesis, Seoul Univ., Seoul, Korea (1981)
  15. Peng Q, Wei Z, Lau B. Medicinal plants research-corni fructus attenuates oxidative stress in macrophages and endothelial cells. Am. J. Chinese Med. 26: 291-300 (1998) https://doi.org/10.1142/S0192415X98000336
  16. Chung HR, Lee JY, Kim DC, Hwang WI. Senergistic effect of panax ginseng and cinnamoum blume mixture on the inhibition of cancer cell growth in vitro. J. Ginseng Res. 23: 99-104 (1999)
  17. Seo KI, Lee SW, Yang KH. Antimicrobial and antioxidative activities of Corni fructus extracts. Korean J. Postharvest Sci. Technol. 6: 99-103 (1999)
  18. Chung SR, Jeune KH, Park SY, Jang SJ. Toxicity and lectins constituents from the seed of Cornus officinalis. Korean J. Pharmacogn. 24: 177-182 (1993)
  19. Yamahara J, Mibu H, Sawada T, Fujimura H, Takino, S. Antidiabetic principles of Corni fructus experimental diabetes induced by streptozotocin. Yakugaku Zasshi 101: 86-90 (1981) https://doi.org/10.1248/yakushi1947.101.1_86
  20. Shen H, Cheng T, Qiao C, Su Z, Li C. Antitumor effect in vitro and immuno-response in vivo of Fructus Mune. Chung Kuo Chung Yao Tsa Chih 20: 365-368 (1995)
  21. Jeng H, Wu CM, Su SJ, Chang WC. A substance isolated from Cornus officinalis enhaces the motility of human sperm. Am. J. Chinese Med. 25: 301-306 (1997) https://doi.org/10.1142/S0192415X97000330
  22. Fukusima M, Fukamiya N, Okano M, Nehira T, Tagahara K, Zhang SX, Zhang DC, Tachibana Y, Bastow K, Lee KH. Cytotoxity of non-alkaloidal taxane diterpenes from taxus chinensis against a paclitaxel-resistant cell line. Jpn. J. Cancer Assoc. 158: 151-154 (2000)
  23. Hwang WI, Cha S. Lee S. Determination of antitumor effects of extracts from korean medicinal plants on cancer cells (L5178Y). Korean J. Biochem. 13: 25-29 (1980)
  24. Kim SH. Apoptic and antimetastatic effect of ursolic acid isolated from Oldenlandia diffusae herbe. J. Taejon Univ. 5: 523-533 (1997)
  25. Lee YC, Kim YE, Lee BY, Kim CJ. Chemical compositions of Corni Fructus and separating properties of its flesh by drying. Korean J. Food Sci. Technol. 24: 447-450 (1992)
  26. Kim DY, Choi JW, Park JC, Lee CK. Effect of ursolic acid and hyperin on neurotransmilters related compouns in brain tissue in vitro. Korean J. Pharmacogn. 29: 187-192 (1998)