Anticoagulant Activities of Brown Seaweed Extracts in Korea

국내산 주요 갈조류 추출물의 항혈액응고 활성

  • Published : 2004.12.31


Effects of extraction conditions and molecular fractionation on anticoagulant activities of major brown seaweeds in Korea were investigated. Hot water extracts of C. costata, U. pinnatifida (Sporophyte), L. japonica, K. crassifolia, E. stolonifera, E. bicyclis, S. horneri, and E. kurome increaced activated partial thromboplastin time (APTT) over 190 seconds, which may be related to intrinsic pathway of blood coagulation. Hot water extract of E. Kurome (EKJ) was further fractionated by ethanol precipitation. EKJ-eim, ethanol-insoluble material of EKJ, showed higher anticoagulant activity than EKJ. EKJ-eim was further fractioned with ultrafiltration. EKJ-eim 1, (over 100 kDa) fraction showed higher APTT activity than EKJ-eim. A EKJ-eim 1 was sulfated polysaccharide consisting of fucose, xylose, mannose, galactose, glucose and, sulfate at molar ratio of 1 : 0.05 : 0.10 : 0.15 : 0.17 : 1.46. The anticoagulant activity increased as sulfate content and molecular weight increased.


  1. Ito K, Hori K. Seaweed: chemical composition and potential food uses. Food Rev. Int. 5: 101-144 (1989)
  2. Shim YY, An JH, Cho WD, Chun H, Kim KI, Cho HY, Yang HC. Inhibitory mechanism of blood coagulation and in vivo anticoagulant activities of polysaccharides isolated from Codium fragile. J. Korean Soc. Food Sci. Nutr. 31: 917-923 (2002)
  3. Kweon MH, Park MK, Ra KS, Sung HC, Yang HC. Screening of anticoagulant polysaccharides from edible plants. Agric. Chem. Biotech. 39:159-164 (1996)
  4. Dubois M, Gills KA, Hamilton JK, Rebers PA, Smith F. Colorimetric method for the determination of sugars and related substances. Anal. Chem. 28: 350-352 (1956)
  5. Koo JG, Jo KS, Do JR, Woo SJ. Isolation and purification of fucoidans from Laminaria religiosa and Undaria pinnatifida in korea. J. Korean Fish. Soc. 28: 227-236 (1995)
  6. Yoon JA, Yu KW, Jun WJ, Cho HY, Son YS, Yang HC. Screening of anticoagulant activity in the extracts of edible seaweeds and optimization of extraction condition. J. Korean Soc. Food Sci. Nutr. 29: 1098-1106 (2000)
  7. Lowry OH, Rosebrough NJ, Farr L, Rindall RJ. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193: 256-275 (1951)
  8. Nishino T, Yokoyama G, Dobashi K, Fujihara M, Nagumo T. Isolation, purification, and characterization of fucose containing sulfated polysaccharides from the brown seaweed Ecklonia kurome and their blood-anticoagulant activities. Carbohydr. Res. 186: 119-129 (1989)
  9. Richard L, Mueller MD. History of drugs for thrombotic disease. Circulation 89: 432-450 (1994)
  10. Dodgson KS, Price RG. A note on the determination of the ether sulphate content of sulphated polysaccharides. J. Biochem. 84: 106-107 (1962)
  11. Percival E, McDowell RH. Chemistry and Enzymology of Marine Algal Polysaccharides. Academy Press Inc. London, UK (1967)
  12. Kim HS, Nam KH, Lee DH. Measurement of PT, APTT, and fibrinogen by automatic coagulation analyzer, sysmex CA-540. J. Clin. Pathol. Qual. Cont. 23: 247-252 (2001)
  13. Blumenkronz N, Asboe-Hansen G. New method for quantitative determination of uronic acids. Anal. Biochem. 54: 484-487 (1973)
  14. Jaques LB. Heparins: anionic polyelectroylte drugs. Pharmacol. Exp. Therap. 31: 99-166 (1979)
  15. Furneaux RH, Miller IJ, Stevenson TT. Agaroids from New Zealand members of the Gracilariaceae (Gracilariales, Rhodophyta)- a novel dimethylated agar. Hydrobiologia 204: 645-650 (1990)
  16. Nishino T, Nagumo T. The sulfate-content dependence of the anticoagulant activity of a fucan sulfate from the brown seaweed Ecklonia kurome. Carbohydr. Res. 214: 193-197 (1991)
  17. Jackson CM, Nemerson Y. In Blood Coagulation. Ann. Rev. Biochem. 49: 765-771 (1980)
  18. Park MK, Kweon MH, Cho HY, Yang HC. Anticoagulant activity of sulfated polysaccharides isolated from Codium fragile. J. Korean Soc. Agric. Chem Biotech. 42: 140-146 (1999)