Acetylcholinesterase Inhibitory Activity and Protective Effect against Cytotoxicity of Perilla Seed Methanol Extract

들깨 메탄올 추출물의 acetylcholinesterase 억제활성 및 세포독성 보호효과

  • Published : 2004.12.31

Abstract

Acetylcholinesterase inhibitory activity and protective effect against cytotoxicity of PC 12 cell induced by beta-amyloid protein and glutamate were examined in perilla seed methanol extract and its solvent fractions. Methanol extract of perilla seed showed dose-dependent acetylcholinesterase inhibitory activity, with n-butanol fraction showing strongest activity. Perilla seed methanol extract also decreased glutamate- and ${\beta}-amyloid$ protein $(A{\beta})-induced$ cytotoxicities of PC 12 cells dose-dependently. Formation of TBARS induced by $FeSO_{4^-}H_2O_2$ in rat brain was significantly reduced by perilla seed methanol extract, with strongest protective activity formation of TBARS shown in n-butanol fraction. Results suggest perilla seed methanol extract may attenuate actylcholinesterase activity and cytotoxicity induced by glutamate and ${\beta}-amyloid$ protein through suppression of oxidative stress.

Keywords

perilla seed;acetylcholinesterase;cytotoxicity;glutamate;${\beta}-amyloid$ protein

References

  1. Selkoe DJ. The molecular pathology of Alzheimer's disease. Neuron. 6: 487-498 (1991) https://doi.org/10.1016/0896-6273(91)90052-2
  2. Karp F, Mihaliak CA, Harri JL, Croteau R. Monoterpene biosynthesis specificity of the hydroxylactions of (-)-limonene by enzyme preperations from peppermint (Menta pipertia), spearmint (Mentha spicata), and perilla (Perilla frutescens) leaves. Arch Biochem. Biophys. 276: 219-226 (1990) https://doi.org/10.1016/0003-9861(90)90029-X
  3. Carmichael J, DeGraff WG, Gazdar AF, Minna JD, Mitchell JB. Evaluation of tetrazolium-based semiautomated colorimetric assay: Assesment of chemosensitivity testing. Cancer Res. 47: 936-942 (1987)
  4. Giacobini E. Cholinesterase inhibitors do more than inhibit cholinesterase. pp. 227-235. In: Cholinesterase and cholinesterase inhibitors. Giacobini E (ed.), Martin Dunitz, London, UK (2000)
  5. Bondy SC, Lee DK. Oxidative stress induced by glutamate receptor agonist. Brain Res. 610: 229-233 (1993) https://doi.org/10.1016/0006-8993(93)91405-H
  6. Paradis E, Douillard H, Koutroumanis M, Goodyer C, LeBlanc A. Amyloid peptide of Alzheimer's disease downregulates Bcl-2 and upregulate Bax expression in human neurons. J. Neurosci. 16: 7533-7539 (1996) https://doi.org/10.1523/JNEUROSCI.16-23-07533.1996
  7. Choi YT, Jung CH, Lee SR, Bae JH, Suh MH, Park JH, Park CW, Suh SI. The green tea polyphenol epigallocatechin gallate attenuates $\beta$-amyloid induced neurotoxicity in cultured hippocampal neuron. Life Sci. 70: 603-614 (2001) https://doi.org/10.1016/S0024-3205(01)01438-2
  8. Yan JJ, Cho JY, Kim HS, Kim KL, Jung JS, Huh SO, Suh HW,Kim YH, Song DK. Protective effects against $\beta$-amyloid peptide toxicity in vivo with long term administration of ferulic acid. Bri. J. Pharm. 133: 89-96 (2001) https://doi.org/10.1038/sj.bjp.0704047
  9. Talesa VN. Acetylcholinesterase in Alzheimer's disease. Mech Aging Dev. 122: 1961-1969 (2001) https://doi.org/10.1016/S0047-6374(01)00309-8
  10. Osakada F, Hashino A, Kume T, Katsuki H, Kaneko S, Akaike A. Neuroprotective effects of alpha-tocopherol on oxidative stress in rat striatal cultures. Eur. J. Pharmacol. 465: 15-22 (2003) https://doi.org/10.1016/S0014-2999(03)01495-X
  11. Price DL, Tanzi RE, Borchelt DR, Sisodia SS. Alzheimer's disease: genetic studies and transgenic models. Annu. Rev. Genet. 32: 461-493 (1998) https://doi.org/10.1146/annurev.genet.32.1.461
  12. Behl C, Davis JB, Cole GM, Schubert D. Vitamin E protects nerve cells from amyloid $\beta$-protein toxicity. Biochem. Biophys. Res. Commun. 186: 944-950 (1992) https://doi.org/10.1016/0006-291X(92)90837-B
  13. Colom LV, Diaz ME, Beers DR, Neely A, Xie WJ, Appel SH. Role of potassium channels in amyloid induced cell death. J. Neurochem. 70: 1924-1934 (1998)
  14. Harada J, Sugimoto M. Activation of caspase-3 in $\beta$-amyloidinduced apoptosis of cultured rat cortical neurons. Brain Res. 842: 311-323 (2000) https://doi.org/10.1016/S0006-8993(99)01808-9
  15. Yan XZ, Qiao JT, Dou Y, Qiao ZD. Beta-amyloid peptide fragment 31-35 induces apoptosis in cultured cortical neurons. Neurosci. 92: 177-184 (1999) https://doi.org/10.1016/S0306-4522(98)00727-1
  16. Jenike MA, Albeit MS, Baer L. Oral phytostigmine as treatment for dementia of the Alzheimer's type: a long term outpatient trial. Alzheimer's Dis. Assoc. Disod. 4: 226-231 (1990)
  17. Kurowska EM, Dresser GK, Deutsch L, Vachon D, Khalil W. Bioavailability of omega-3 essential fatty acids from perilla seed oil. Prostaglandins Leukotrienes Essential Fatty Acids 68: 207- 212 (2003) https://doi.org/10.1016/S0952-3278(02)00271-5
  18. Behl C, Hovery L, Krajewski S, Schubert D, Reed JC. Bcl-2 prevents killing of neuronal cells by glutamate but not by amyloid beta protein. Biochem. Biophys. Res. Commun. 197: 949-955 (1993) https://doi.org/10.1006/bbrc.1993.2571
  19. Fukuda Y, Namiki M. Recent studies on sesame seed and oil. J. Jpn. Soc. Food Sci. Tech. 35: 552-559 (1988) https://doi.org/10.3136/nskkk1962.35.8_552
  20. Choi KG. The long-term care and dementia policy in welfare states. Soc. Welfare Policy 17: 55-75 (2003)
  21. Tanzi RE, Gaston S, Bush A, Romano D, Pettingell W, Peppercorn J, Paradis M, Gurubhagavatula S, Jenkins B, Wasco W. Genetic-heterogenity of gene defects responsible for familial Alzheimer-disease. Genetica 91: 255-263 (1993) https://doi.org/10.1007/BF01436002
  22. Sclar DA, Skaer TL. Current concepts in the treatment of Alzheimer's disease. Clin. Ther. 14: 2-10 (1992)
  23. Mattson MP, Barger SW, Cheng B, Lieberburg I, Smith-Swintosky VL, Rydel RE. $\beta$-Amyloid precursor protein metabolites and loss of neuronal $Ca^{2+}$ homeostasis in Alzheimer's disease. Trends Neurosci. 16: 409-414 (1993) https://doi.org/10.1016/0166-2236(93)90009-B
  24. Goodman Y, Mattson MP. Secreted forms of $\beta$-amyloid precursor protein protect hippocampal neurons against amyloid $\beta$-peptideinduced oxidative injury. Exp. Neurol. 128: 1-12 (1994) https://doi.org/10.1006/exnr.1994.1107
  25. Selkoe DJ. Toward a comprehensive theory for Alzheimer's disease. Hypothesis: Alzheimer's disease is caused by the cerebral accumulation and cytotoxicity of amyloid beta-protein. Ann. N.Y. Acad. Sci. 924: 17-25 (2000) https://doi.org/10.1111/j.1749-6632.2000.tb05554.x
  26. Park WK, Park BH, Park YH. Encyclopedia of food and food science. Shin Kwang Publishing Co. Seoul, Korea. p. 243 (2000)
  27. Seyfried J, Evert BO, Rundfeldt C, Schulz J, Karl B, Kovar A, Klockgether T, Wullner U. Flupirtine and retigabine prevent Lglutamate toxicity in rat pheochromocytoma PC12 cells. Eur. J. Pharmacol. 400: 155-166 (2000) https://doi.org/10.1016/S0014-2999(00)00397-6
  28. Jang JH, Surh YJ. Protective effect of resveratrol on $\beta$-amyloidinduced oxidative PC12 cell death. Free Radic. Biol. Med. 34: 1100-1110 (2003) https://doi.org/10.1016/S0891-5849(03)00062-5
  29. Tada M, Matsumoto R, Yamaguchi H, Chiba K. Novel antioxidants isolated from perilla Frutescens britton var. crispa (Thunb). Biosci. Biotech. Biochemi. 60: 1093-1095 (1996) https://doi.org/10.1271/bbb.60.1093
  30. Hardy J. Amyloid, the presenilins and Alzheimer's disease. Trends Neurosci. 20: 154-159 (1997) https://doi.org/10.1016/S0166-2236(96)01030-2
  31. Ellman GL, Courtney KD, Andres JV, Featherstone RM. A new and lapid colormetric determination of acetylcholinesterase activity. Biochem. Pharmacol. 7: 88-95 (1961) https://doi.org/10.1016/0006-2952(61)90145-9
  32. Nagatsu A, Tenmaru K, Matsuura H, Murakami N, Kobayashi T, Okuyama H, Sakakibara J. Novel antioxidants from roasted perilla seeds. Chem. Pharm. Bull. 43: 887-889 (1995) https://doi.org/10.1248/cpb.43.887
  33. Lee YJ, Shin DH, Chang YS, Shin JI. Antioxidative effects of some edible plant solvent extracts with various synergist. Korean J. Food Sci. Technol. 25: 683-688 (1993)
  34. Selkoe DJ. Translating cell biology into therapeutic advances in Alzheimer's disease. Nature 399: A23-A31 (1999) https://doi.org/10.1038/19866
  35. Pederson WA, Kloczewiak MA, Bluszajin JK. Amyloid beta-protein reduces acetylchoin synthesis in a cell line derived from chlinergic neurons of the basal forebrain. Proc. Natl. Acad. Sci. USA. 93: 8068-8071 (1996) https://doi.org/10.1073/pnas.93.15.8068
  36. Trabace L, Cassano T, Steardo L, Pietra C, Villetti G, Kendrick KM, Cuomo V. Biochemical and neurobeabavioral profile of CHF2819, a novel, orally active acetylcholinesterase inhibitor for Alzheimer's disease. J. Pharmacol. Exp. Ther. 294: 187-194 (2000)
  37. Naito M, Umegaki H, Iguchi A. Protective effect of probucol against glutamate induced cytotoxicity in neurinal cell line PC12. Neurosci. lett. 186: 211-213 (1995) https://doi.org/10.1016/0304-3940(95)11321-M
  38. Puttfarcken PS, Getz RL, Coyle JT. Kainic acid-induced lipid peroxidation: protection with butylated hydroxytoluen and U78517F in primary cultures of cerebella grannule cells. Brain Res. 624: 223-232 (1993) https://doi.org/10.1016/0006-8993(93)90081-W