DOI QR코드

DOI QR Code

Micro Cell Counter Integrated with An Oxygen Micropump

산소 미세 펌프가 내장된 미세 세포 계수기

  • 손상욱 (한국과학기술원 기계공학과) ;
  • 최요한 (한국과학기술원 기계공학과) ;
  • 이승섭 (한국과학기술원 기계공학과)
  • Published : 2004.08.01

Abstract

This paper describes fabrication of a micro cell counter integrated with an oxygen micropump and counting experiment with Sephadex G-25 beads ($70{\sim}100\;{\mu}m$). The pumping part consisted of a microheater, catalyst (manganese dioxide) enveloped with paraffin, hydrogen peroxide, and microchannel, and the counting part consisted of collimated light, a microwindow, and a phototransistor including an external circuit. The micropump generated oxygen gas by decomposing hydrogen peroxide with manganese dioxide, which was initiated by melting the paraffin with the microheater, and pumped beads in the microchannel. When the beads passed the microwindow, they shaded the collimated light and changed the illumination on the phototransistor, which caused the current variation in the circuit. The signals, according to the bead size, reached up to 22 mV with noise level of 2 mV during 50 seconds and the numbers of peaks were analyzed by magnitude.

Keywords

Micro Cell Counter;Oxygen Micropump;Microchannel;Sizing

References

  1. Larsen, U.D., Blankenstein, G. and Branebjerg, J., 1997, 'Microchip Coulter Particle Counter,' Proc. Int. Conf. On Solid-State Sensors and Actuators (Transducers '97), pp. 1319-1322 https://doi.org/10.1109/SENSOR.1997.635479
  2. Gawad, S., Schild, L. and Renaud, Ph., 2001, 'Micromachined Impedance Spectroscopy Flow Cytometer for Cell Analysis and Particle Sizing,' Lab on a Chip, Vol. 1, pp. 76-82 https://doi.org/10.1039/b103933b
  3. FACSVantage SE User's Guide, Becton, Dickinson and Company, USA, p. 176
  4. Onoda, H., Takamura, Y. and Horiike, Y. 2002, 'Electric Damage Free Separation Chip of Lymphocytes Sub-Unit Employing High Pressure Electroosmosis Pump,' Proc. Int. Conf. On Micro Total Analysis Systems $({\mu}JTAS'02),$ pp. 955-957
  5. Wolff, A., Perch-Nielsen, I. R., Larsen, U. D., Friis, P., Goranovic, G., Poulsen, C. R., Kutter, J. P. and Telleman, P., 2003, 'Integrating Advanced Functionality in a Microfabricated High-Throughput Fluorescent-Activated Cell Sorter,' Lab on a Chip, Vol 3, pp. 22-27 https://doi.org/10.1039/b209333b
  6. Roberts, K., Parameswaran, M., Moore, M. and Muller, R.S., 1999, 'A Silicon Microfabricated Aperture for Counting Cells Using the Aperture Impedance Technique,' Proc. IEEE Canadian Conf. On Electrical and Computer Engineering, pp. 1668-1673 https://doi.org/10.1109/CCECE.1999.804968
  7. Altendorf, E., Zebert, D., Holl, M. and Yager, P., 1997, 'Differential Blood Cell Counts Obtained Using a Microchannel Based Flow Cytometer,' Proc. Int. Conf. On Solid-State Sensors and Actuators (Transducers '97), pp. 531-534 https://doi.org/10.1109/SENSOR.1997.613704
  8. Carlen, E. T. and Mastrangelo, C. H., 2000, 'Paraffin Actuated Surface Micromachined Valves,' Proc. IEEE Int. Conf. On Micro Electro Mechanical Systems (MEMS'00), pp. 381-385 https://doi.org/10.1109/MEMSYS.2000.838547
  9. Wang, X., Zhou, Z., Ye, X., Li, Y. and Zhang, W., 1998, 'A PZT-Driven Micropump,' Proc. Int. Sym. On Micromechatronics and Human Science, pp. 269-272 https://doi.org/10.1109/MHS.1998.745797
  10. Chujo, H., Matsumoto, K. and Shimoyama, I., 2003, 'A High Flow Rate Electroosmotic Pump with Small Channels in Parallel,' Proc. IEEE Int. Conf. On Micro Electro Mechanical Systems (MEMS'03), pp. 351-354 https://doi.org/10.1109/MEMSYS.2003.1189758
  11. Morgan, H., Green, N. G., Hughes, M. P., Monaghan, W. and Tan, T. C., 1997, 'Large-Area Travelling-Wave Dielectrophoresis Particle Separator,' J. Micromech. Microeng., Vol. 7, pp. 65-70 https://doi.org/10.1088/0960-1317/7/2/004
  12. Hatch, A., Kamholz, A. E., Holman, G., Yager, P., and Bohringer, K. F., 2001, 'A Ferrofluidic Magnetic Micropump,' J. Microelectromech. Sys., Vol. 10, No. 2, pp. 215-221 https://doi.org/10.1109/84.925748
  13. Jang, J. and Lee, S.S., 2000, 'Theoretical and Experimental Study of MHD (Magnetodydro Dynamic) Micropump,' Sen. and Act. A, Vol. 80, pp. 84-89 https://doi.org/10.1016/S0924-4247(99)00302-7
  14. Xie, J., He, Q., Tai, Y.-C., Liu, J. and Lee, T., 2002, 'Integrated Electrospray Chip for Mass Spectrometry,' Proc. Int. Conf. On Micro Total Analysis Systems $({\mu}JTAS'02),$ pp. 709-711
  15. Yo Han Choi, Sanguk Son and Seung S. Lee, 2003, 'Novel Micropump Using Oxygen as Pumping Source,' Proceedings of the 16th IEEE MEMS conference, pp. 116-119 https://doi.org/10.1109/MEMSYS.2003.1189701
  16. Chien-Chong Hong, Suresh Murugesan, Sanghyo Kim, Gregory Beaucage, Jin-Woo Choi and Chong H. Ahn, 2003, 'A Functional On-chip Pressure Generator Using Solid Chemical Propellant for Disposable Lab-on-a-chip,' Proceedings of the 16th IEEE MEMS conference, pp. 16-19 https://doi.org/10.1109/MEMSYS.2003.1189676
  17. Son, S. U., Choi, Y. H. and Lee, S. S., 2003, 'Fabrication of Micro Cell Counter Integrated with Oxygen Pump,' Proc. Korean MEMS Conf., pp. 436-440
  18. Chang, R., 1981, 'Chemistry,' 2nd ed., Random House, New York, p. 63, p. 591
  19. Atanasov, P. A. and Imamova, S. E., 2000, 'Optical Parameters of Silicon Carbide and Silicon Nitride Ceramics in 0.2-1.3 ${\mu}m$ Spectral Range,' J. Applied physics, Vol. 88, No.8, pp. 4671-4675 https://doi.org/10.1063/1.1311302
  20. Son, S. U. and Lee, S. S., 2003, 'Narrowing Microfluid Width in Microchannel Using Air Boundaries,' Proc. Int. Conf. On Micro Total Analysis Systems $({\mu}TAS'03),$ pp. 147-150