DOI QR코드

DOI QR Code

A Novel Cell Count Method Using Micro Lattice Engraved On A Culture Dish

미세 격자가 형성된 배양 접시를 이용한 새로운 세포 계수 방법

  • 손상욱 (한국과학기술원 기계공학과) ;
  • 최요한 (한국과학기술원 기계공학과) ;
  • 이승섭 (한국과학기술원 기계공학과)
  • Published : 2004.08.01

Abstract

A novel cell count method, which can improve the count efficiency and reduce contamination problem, was presented using micro lattice engraved on culture dish. The micro lattice has feature of $50{\mu}m{\times}50{\mu}m$ rectangular shape, $2{\mu}m$ line width, and $2{\mu}m$ depth in $3mm{\times}3mm$ area. In this paper, nickel mold was fabricated with thickness of 3mm and diameter of 80 mm, and transcription of the micro lattice on a polystyrene cell culture dish was performed by hot embossing at $200\;^{\circ}C$. The tedious and error-prone harvest/load processes of conventional cell counts with a hemocytometer could be omitted, and these advantages became magnified during periodical counts involving long-term cultures. SupT1 cells and HeLa cells were cultivated with the dish for 7 days in $CO_2$ incubator and counted as $371.84/mm^2$ and $123.36/mm^2$, respectively, during the cultivation without harmful effects on the cells.

Keywords

Cell Count;Culture Dish;Micro Lattice;Hemo-cytomter;Hot Embossing

References

  1. Coulter, W. H., 1956, 'High Speed Automatic Blood Cell Counter and Cell Size Analyzer,' Proc. National Elecuonics Conf. 12, pp.1034-1040
  2. Han, K. -H., Landers, J. P. and Frazier, A. B., 2003, 'Continuous Paramagnetophoretic Microseparator for Blood Cells,' Proc. Int. Conf. On Solid-State Sensors and Actuators (Transducers'03), pp. 1129-1232 https://doi.org/10.1109/SENSOR.2003.1216994
  3. Sergent-Tanguy, S., Chagneau, C., Neveu, I. and Naveilhan, P., 2003, 'Fluorescent Activated Cell Sorting (FACS): a Rapid and Reliable Method to Estimate the Number of Neurons in a Mixed Population,' J. Neuroscience Methods, Vol. 129, pp. 73-79 https://doi.org/10.1016/S0165-0270(03)00210-3
  4. Asai, J., Suzuki, R, Fujimoto, T., Nagashima, G, Miyo, T., Takahashi, M. and Hokaku, H., 1997, 'Fluorescent Automatic Cell Sorter (FACS) and Irrnnunohistological Investigations of CD-68 Positive Mononuclear Cell Infiltrates in Meningiomas,' Clinical Neurology and Neurosurgery, Vol. 99, pp. S133-S134 https://doi.org/10.1016/S0303-8467(97)81874-4
  5. Benjumovich, M. S., 2002, 'Use of Poisson Units in Radiology,' Radiation Physics and Chemistry, Vol. 66, pp. 7-9 https://doi.org/10.1016/S0969-806X(02)00279-7
  6. Tezcaner, A., Bugra, K. and Hasirci, V., 2003, 'Retinal Pigment Epithelium Cell Culture on Surface Modified Poly (hydroxybutyrate-co-hydroxyvalerate) Thin Films,' Biomaterials, Vol. 24, pp. 4573-4583 https://doi.org/10.1016/S0142-9612(03)00302-8
  7. Freshney, R. I., 1994, 'Culture of Animal Cells,' 3rd ed., Wiley-Liss, pp. 243-252
  8. Johnson, D. W., Jeffries, A., Minsek, D. W. and Urditch, R. J., 'Improving the Process Capability of SU-8,' www.microchem.com/resources/materials.htm.
  9. Han, M. H., Huh, J. -K., Lee, S. S. and Lee, S. -J., 2002, 'Fabrication of a Micro-Riblet Film Using MEMS Technology and Its Application to Drag Reduction,' Transactions of the KSME, B, Vol. 26, No.7, pp. 991-996
  10. Jo, B. -H., Van Lerberghe, L. M., Motsegood, K. M. and Beebe, D.J., 2000, 'Three-Dimensional MicroChannel Fabrication in Polydimethylsiloxane (PDMS) Elastomer,' J. Microelectromech. Sys., Vol. 9, No.1, pp.76-81 https://doi.org/10.1109/84.825780
  11. Kaji, H., Nishizawa, M. and Matsue, O., 2003, 'Localized Chemical Stimulation to Micropatterned Cells Using Multiple Laminar Fluid Flows,' Lab on a chip, Vol. 3, pp. 208-211 https://doi.org/10.1039/b304350a
  12. Ephrussi, A. and Pourquie, O., 2003, 'Pattern Formation and Developmental Mechanisms from Cell Patterning to Organogenesis,' Current Opinion in Genetics & Development, Vol. 13, pp. 323-325 https://doi.org/10.1016/S0959-437X(03)00092-3
  13. Li, M., Glawe, J. D., Green, H., Mills, D. K. and Mcshane, M. J. 2000, 'Effect on High-Aspect-Ratio Microstructures on Cell Growth and Attachment,' Int. IEEE-EMBS Topic Conf. On Microtech. In Medicine & Biology, pp. 531-536 https://doi.org/10.1109/MMB.2000.893841

Cited by

  1. Guided cell growth through surface treatments vol.19, pp.11, 2005, https://doi.org/10.1007/BF02916509