Evaluation of maternal toxicity in rats exposed to the insecticide amitraz during pregnancy

임신 중 살충제 amitraz에 노출된 랫드의 모독성 평가

  • Shin, Jin-Young (College of Veterinary Medicine, Chonnam National Universiyt) ;
  • Oh, Ki-Suk (College of Veterinary Medicine, Chonnam National Universiyt) ;
  • Shin, Dong-Ho (College of Veterinary Medicine, Chonnam National Universiyt) ;
  • Kim, Sung-Ho (College of Veterinary Medicine, Chonnam National Universiyt) ;
  • Kim, Hyoung-Chin (Korea Research Institute of Bioscience and Biotechnology) ;
  • Park, Seung-Chun (College of Veterinary Medicine, Kyungpook National University) ;
  • Lee, Hyun-Sook (Korea Institute of Toxicology, KRICT) ;
  • Chung, Moon-Koo (Korea Institute of Toxicology, KRICT) ;
  • Kim, Jong-Choon (College of Veterinary Medicine, Chonnam National Universiyt)
  • 신진영 (전남대학교 수의과대학) ;
  • 오기석 (전남대학교 수의과대학) ;
  • 신동호 (전남대학교 수의과대학) ;
  • 김성호 (전남대학교 수의과대학) ;
  • 김형진 (한국생명공학연구원) ;
  • 박승춘 (경북대학교 수의과대학) ;
  • 이현숙 (한국화학연구원 부설 안전성평가연구소) ;
  • 정문구 (한국화학연구원 부설 안전성평가연구소) ;
  • 김종춘 (전남대학교 수의과대학)
  • Accepted : 2004.09.15
  • Published : 2004.12.30

Abstract

The present study was carried out to investigate the potential adverse effects of amitraz on pregnant dams after maternal exposure during the gestational days (GD) 1 through 19 in Sprague-Dawley rats. The test chemical was administered orally to pregnant rats at dose levels of 0, 3, 10, or 30 mg/kg/ day. During the test period, clinical signs, mortality, body weights, food consumption, serum biochemistry, gross findings, organ weights and reproductive findings on GD 20 were examined. In the 30 mg/kg group, an increase in the incidence of abnormal clinical signs and death, a suppression in the body weight gain, and a decrease in the food consumption were observed. A decrease in the liver weight and increases in the kidneys, adrenal glands and heart weights were also found. Serum biochemical investigations revealed increases in the aspartate aminotransferase (AST), total bilirubin, and chloride. In addition, an increase in the fetal death and decreases in the litter size and fetal body weight were seen at caesarean section. Inthe 10 mg/kg group, an increase in the incidence of abnormal clinical signs, decreases in the food consumption and liver weight, increases in the total bilirubin and chloride, and a decrease in the fetal body weight were observed. There were no adverse effects on clinical signs, mortality, body weights, food consumption, serum biochemistry, gross findings, organ weights and reproductive findings in the 3 mg/kg group. Based on the results, it was concluded that the 19-day repeated oral dose of amitraz to pregnant rats caused increases in the clinical signs, kidneys, adrenal glands and heart weights, AST, total bilirubin and chloride and decreases in the body weight gain, food consumption and liver weight at the dose levels of above 10 mg/kg/day. Under the present experimental conditions, the no-observed-adverse-effect level (NOAEL) of amitraz was considered to be 3 mg/kg/day.

Acknowledgement

Supported by : 전남대학교

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