Optimization of the Viability of Probiotics in a Fermented Milk Drink by the Response Surface Method

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Chen, Ming-Ju;Chen, Kun-Nan;Lin, Chin-Wen

  • 투고 : 2003.08.09
  • 심사 : 2004.01.27
  • 발행 : 2004.05.01

초록

Growth promoters were added to skim milk to retain the viability of Lactobacillus acidophilus and Bifidobacterium longum to help the product meet the "therapeutic minimum" at the time of consumption. The experiments were divided into two parts. The first part of the study used chicory inulin, isomalto-oligosaccharides and sucrose to investigate the effects of sugars on the activity of L. acidophilus and B. longum. The results indicated that the addition of isomalto-oligosaccharides stimulated growth of L. acidophilus and B. longum, resulting in a higher level of the probiotics after one month storage and yielded better $\beta$-galactosidase activity during fermentation. The second part studied the effects of three growth promoters on the viability of the probiotic cultures and the response surface method was employed to find the optimal ratio for addition of the growth promoters. The optimal ratio for added calcium gluconate, sodium gluconate and N-acetylglucosamine in fermented milk drinks were established. The response surface method proved to be a very effective way of optimizing the activity of probiotic cultures when developing a new fermented milk drink.

키워드

Probiotics;Response Surface Method;Fermented Milk

참고문헌

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피인용 문헌

  1. 1. Optimization of Incorporated Prebiotics as Coating Materials for Probiotic Microencapsulation vol.70, pp.5, 2006, doi:10.5713/ajas.2004.705
  2. 2. Increased shelf life of a bioherbicide through combining modified atmosphere packaging and low temperatures vol.17, pp.4, 2007, doi:10.5713/ajas.2004.705
  3. 3. Development of an Oriental-style dairy product coagulated by microcapsules containing probiotics and filtrates from fermented rice vol.60, pp.1, 2007, doi:10.5713/ajas.2004.705
  4. 4. PROCESS OPTIMIZATION FOR A NOVEL KEFIR CANDY WITH HIGH PROBIOTIC VIABILITY vol.34, pp.2, 2011, doi:10.5713/ajas.2004.705

과제정보

연구 과제 주관 기관 : National Science Council