Serotype, antimicrobial susceptibility and plasmid profile of Listeria monocytogenes isolated from livestock products and product processing plants

축산물 및 작업장 유래 Listeria monocytogenes의 혈청형, 약제감수성 및 plasmid profile

  • Park, Sang-koo (College of Veterinary Medicine/Institute of Animal Medicine, Gyeongsang National University) ;
  • Son, Won-geun (Department of Veterinary Medicine, Cheju National University) ;
  • Lee, Hu-jang (College of Veterinary Medicine/Institute of Animal Medicine, Gyeongsang National University) ;
  • Kim, Young-hwan (College of Veterinary Medicine/Institute of Animal Medicine, Gyeongsang National University) ;
  • Kang, Ho-jo (College of Veterinary Medicine/Institute of Animal Medicine, Gyeongsang National University)
  • 박상구 (경상대학교 수의과대학/동물의학연구소) ;
  • 손원근 (제주대학교 수의학과) ;
  • 이후장 (경상대학교 수의과대학/동물의학연구소) ;
  • 김용환 (경상대학교 수의과대학/동물의학연구소) ;
  • 강호조 (경상대학교 수의과대학/동물의학연구소)
  • Accepted : 2004.03.05
  • Published : 2004.03.31

Abstract

This study was carried out to investigate the serotype, and antimicrobial susceptibility and analyze the plasmid profile for the 145 isolates of L. monocytogenes isolated from livestock products and these product processing plants in Gyeongnam, Korea. All of L. monocytogenes strains belonged to serotype 1/2b (57.9%), 1/2a (20.0%), 4b (11.4%), 1/2c, 3b, 4c (each 2.9%) and 4d (0.7%). Serotype 1/2b, 1/2a, 4b from each source were found predominantly. Serotype 1/2b was predominantly higher than other serotype, and there was no significant difference between serotypes isolated from livestock products and product processing plants. 4b was major serotype isolated from raw milk and pork, and serotypes isolated from beef, chickens and slaughterhouse were 1/2b and 1/2a. The susceptibility of 145 strains of L. monocytogenes to 14 antibiotics commonly used in veterinary and human therapy was determined by disk diffusion method. All of L. monocytogenes strains were susceptible to amikacin, ampicillin, cephalothin, chloramphenicol, gentamicin, kanamycin, neomycin and penicillin. L. monocytogenes strains had the highest resistance with colistin (100%), oxytetracycline (44.8%), tetracycline (43.4%) followed by erythromycin (2.8%), spectinomycin (1.4%) and streptomycin (0.7%). Tetracycline resistance, and serotype distribution of the isolates from sample sources were significantly different. Resistance to at least one antibiotic was observed in all of them and 7 different resistant profiles were recorded. The most common resistance pattern were CL-OTC-TC (colistin-oxytetracycline-tetracycline) (42.8%). Among all tested isolates, two different plasmid profiles were observed. Of the 97 examined strains, 14 (14.4%) contained either the 8 and 11 kb plasmid or the 11 kb.

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