Proteome analysis: Salmoenlla enteritidis antigen proteins

Proteomics 기법을 이용한 Salmonella enteritidis의 항원 단백질 분석

  • Park, Mi-rim (Research Institute of Life Science, College of Veterinary Medicine, Gyeongsang National University) ;
  • Shin, Yong-seung (Research Institute of Life Science, College of Veterinary Medicine, Gyeongsang National University) ;
  • Han, Dae-yong (Research Institute of Life Science, College of Veterinary Medicine, Gyeongsang National University) ;
  • Kim, Yong-hwan (Research Institute of Life Science, College of Veterinary Medicine, Gyeongsang National University) ;
  • Jung, Tae-sung (Research Institute of Life Science, College of Veterinary Medicine, Gyeongsang National University) ;
  • Lee, Hu-Jang (Research Institute of Life Science, College of Veterinary Medicine, Gyeongsang National University) ;
  • Lee, Eung-Goo (SungGyunKwan University) ;
  • Kim, Jong-su (Research Institute of Life Science, College of Veterinary Medicine, Gyeongsang National University) ;
  • Kim, Eun-hee (Jinju International University) ;
  • Kim, Gon-Sup (Research Institute of Life Science, College of Veterinary Medicine, Gyeongsang National University)
  • 박미림 (경상대학교 수의과대학(생명과학연구소)) ;
  • 신용승 (경상대학교 수의과대학(생명과학연구소)) ;
  • 한대용 (경상대학교 수의과대학(생명과학연구소)) ;
  • 김용환 (경상대학교 수의과대학(생명과학연구소)) ;
  • 정태성 (경상대학교 수의과대학(생명과학연구소)) ;
  • 이후장 (경상대학교 수의과대학(생명과학연구소)) ;
  • 이응구 (성균관대학교) ;
  • 김종수 (경상대학교 수의과대학(생명과학연구소)) ;
  • 김은희 (진주국제대학교) ;
  • 김곤섭 (경상대학교 수의과대학(생명과학연구소))
  • Accepted : 2003.10.22
  • Published : 2004.03.31

Abstract

The common pathogen Salmonella enteirtidis (S. enteritidis) is the major cause of foodborne disease. Protein identification by peptide mass fingerprinting using the matrix-assisted laser desorption ionization time of fight (MALDI-TOF) mass spectrometry (MS) can analysis unambiguously identity the spots from 2-dimensional electrophoresis (2-DE) gel. In this report, we examined protein components from patterns of S. enteritidis proteins. In addition, antigens that are recognized by sera can be identified by immunoblotting. This study that 2-DE analysis of S. enteritidis yields useful information concerning S. enteritidis proteome, the results that have been obtained led to a more detailed understanding of Salmonella pathology and open further interesting fields for future work.

Acknowledgement

Supported by : KOSEF

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