Quantitative assay of glycosphingolipid and determination of binding affinity for Shigatoxin 2e in pig

돼지의 일령에 따른 glycosphingolipid의 정량 및 Shigatoxin 2e에 대한 친화성 확인

  • Dong, Bun-youn (Korea Green Cross Co.) ;
  • Kim, Sang-Hyun (Department of Pathobiology, Ontario Veterinary College, University of Guelph) ;
  • Cho, Hyun-Ho (National Veterinary Research and Quarantine Service) ;
  • Kim, Yeong-Il (National Veterinary Research and Quarantine Service) ;
  • Lee, Woo-won (Pusan Institute of Health and Environment) ;
  • Kim, Kon-Sup (College of Veterinary Medicine, Research Institute of Life Science, Gyeongsang National University) ;
  • Kang, Ho-Jo (College of Veterinary Medicine, Research Institute of Life Science, Gyeongsang National University) ;
  • Kim, Yong-Hwan (College of Veterinary Medicine, Research Institute of Life Science, Gyeongsang National University)
  • 동분연 (녹십자백신연구소) ;
  • 김상현 (캐나다 구엘프대학교 수의과대학) ;
  • 조현호 (수의과학검역원 서울지소) ;
  • 김영일 (수의과학검역원 부산지소) ;
  • 이우원 (부산시 보건환경 연구원) ;
  • 김곤섭 (경상대학교 수의과대학 생명과학연구소) ;
  • 강호조 (경상대학교 수의과대학 생명과학연구소) ;
  • 김용환 (경상대학교 수의과대학 생명과학연구소)
  • Accepted : 2004.04.17
  • Published : 2004.06.30

Abstract

This study was designed to quantitative assay of cellular receptor globotriosyl ceramide($Gb_3$) and globotetraosyl ceramide($Gb_4$) and to determine binding affinity of the receptors for Shigatoxin 2e in difference with day olds of pig. The amount of $Gb_3$ and $Gb_4$ extracted from each swine tissue were detected in order of spleen, ileocecum, kidney, and jejunum. The amount of $Gb_3$ and $Gb_4$ extracted from each swine ileocecum and jejunum in different day old swine were detected in order of 24, 6, 98, 12 and 35, but the difference of amount was not significant. The binding affinity of $Gb_4$ extracted from ileocecum and jejunum with different day old swine for Stx2e were shown that the extracts of 24, 12, and 35 days old swine were higher significantly than that of 6 and 98 days old swine.

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