Identification and characterization of shiga toxin-producing Escherichia coli isolated from the feces of slaughtered pigs

도축돈 장분변으로부터 Shiga Toxin-Producing Escherchia coli의 분리 와 성상

  • Song, Young-hwan (College of Veterinary Medicine, Chungnam National University) ;
  • Kim, Ji-young (College of Veterinary Medicine, Chungnam National University) ;
  • Chae, Mi-kyung (College of Veterinary Medicine, Chungnam National University) ;
  • Park, Chang-sik (Research Center for Transgenic Cloned Pigs, Chungnam National University) ;
  • Kim, Myung-chul (College of Veterinary Medicine, Chungnam National University) ;
  • Jun, Moo-hyung (College of Veterinary Medicine, Chungnam National University)
  • 송영환 (충남대학교 수의과대학) ;
  • 김지영 (충남대학교 수의과대학) ;
  • 채미경 (충남대학교 수의과대학) ;
  • 박창식 (충남대학교 형질전환복제돼지연구센터) ;
  • 김명철 (충남대학교 수의과대학) ;
  • 전무형 (충남대학교 수의과대학)
  • Accepted : 2004.10.05
  • Published : 2004.12.30

Abstract

Shiga toxin-producing Escherichia coli (STEC) causes various clinical signs in human and animals, and has been indicated as a global enteropathogen with zoonotic importance. In this study, the feces of healthy pigs were collected from the slaughtered pigs of Daejon abattoir during the period from December 2001 to October 2002. Of 326 specimens, 13 STEC were confirmed by culture, PCR and colony hybridization. The isolates were further studied for toxin types, pathogenic factors, plasmid profiles, and antimicrobial resistance to characterize the genetic and toxigenic properties. In PCR, all of 13 isolates were evident to have shiga toxin gene (stx). Of 13 isolates stx1 gene was detected in 4 and stx2 gene in 9. The genes of eaeA, hlyA and rfbE were not present in any isolates. In colony hybridization using shiga toxin common primer (STXc), 2 to 9 per 100 colonies subcultured from 13 isolates showed the positive reaction. In the examination for plasmid profiles of the isolates, one to eleven plasmids with varying sizes of 1.0 Kb to 100 Kb were detected, and the 13 STEC could be classified into four groups by the plasmid patterns. The antimicrobial resistance patterns of the isolates were comparably corresponded with the plasmid profile patterns.

Acknowledgement

Supported by : 한국과학재단

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