Chromosomal band pattern of black-striped field mouse (Apodemus agrarius)

한국 야생 등줄쥐의 고해상도 염색체분염상

  • Oh, Seung-hyun (Laboratory of Departmental Biology and Genomics, College of Veterinary Medicine, Seoul National University) ;
  • Yoon, Yeo-sung (Laboratory of Histology, College of Veterinary Medicine, Seoul National University) ;
  • Jin, Hee-Kyung (Laboratory of Laboratory Animal Medicine, College of Veterinary Medicine, Kyungpook National University) ;
  • Seong, Je-kyung (Laboratory of Departmental Biology and Genomics, College of Veterinary Medicine, Seoul National University)
  • 오승현 (서울대학교 수의과대학 발생.유전학교실) ;
  • 윤여성 (서울대학교 수의과대학 조직학교실) ;
  • 진희경 (경북대학교 수의과대학 실험동물학실) ;
  • 성제경 (서울대학교 수의과대학 발생.유전학교실)
  • Accepted : 2004.02.10
  • Published : 2004.06.30

Abstract

We investigated the cytogenetic characteristics of male black-striped field mouse (Apodemus agrarium) in Korea. Chromosome slides were obtained from blood cell cultures which were synchronized with thymidine blocking or not. In the chromosome slide which synchronization with thymidine blocking was employed on, the GTG(G bands by trypsin using Giemsa)-bands of high resolution were observed. The male black-striped field mouse has 48 chromosomes composed 46 autosomes and XY sex chromosomes. The centromeric regions of autosomes were positive to GTG-banding. According to this investigation, thymidine blocking in cell culture process was useful to get lengthened chromosomes. It may be necessary to employ RBG-banding technique to investigate complementary band patterns between R- and G-banding in black-striped field mouse.

Acknowledgement

Supported by : 한국과학재단

References

  1. Koh, H. S. G- and C- banding pattern analysis of Korean rodents. Korean J. Zool. 1982, 25, 81-92
  2. Lee, H. W., Lee, P. W. and Johnson, K. M. Isolation of the etiologic agent of Korean hemorrhagic fever. J. Infect. Dis. 1978, 137, 298-308
  3. 김윤희, 김희수, 최영현, 이원호. 한국산 등줄쥐의 체세포 및 생식세포 분열에 의한 염색체 분석. 한국실험동물학회지. 2001, 17, 17-26
  4. 조정식, 채갑용, 김지희. 한국에서 서식하는 야생등줄쥐(Apodemus agrarius Pallas)의 실험동물화에 관한 연구(III). 과학기술처, 1993
  5. 김재연, 조정식, 채갑용. 한국에 서식하는 야생등줄쥐의 실험동물화에 관한 연구(I). 과학기술처, 1991
  6. 오승현, 윤영민, 윤여성, 이준섭, 이흥식, 성제경. 한국재래산양의 핵형분석. 대한수의학회지. 1999, 39, 908-920
  7. 土屋公幸. 新しく實驗動物として開發された野生齧齒類の維持方法の開發. 九州實驗動物研究會會報, 1991, 7, 3-9
  8. Oh, S. W., Chae, C. and Jang, D. Spontaneous gastric carcinoid tumors in the striped field mouse (Apodemus agrarius). J. Vet. Med. Sci. 1997, 59, 703-706
  9. Takagi, N. A simple technique to demonstrate the centromeric 'heterochromatin' in the mouse and other animals. Jpn. J. Genetics. 1971, 46, 361-363
  10. 조정식, 채갑용, 김지희. 한국에서 서식하는 야생들줄쥐의 실험동물화에 관한 연구(II). 과학기술처, 1992
  11. Latt, S. A. Microfluorometric detection of deoxyribonucleic acid replication in human metaphase chromosomes.Proc. Natl. Acad. Sci. 1973, 70, 3395-3399
  12. Perry, P. and Wolff, S. New Giemsa method for differential staining of sister chromatids. Nature. 1974, 261, 156-158
  13. Lemieux, N., Drouin, R. and Richer, C. L. Highresolution dynamic and morphological G-bandings (GBG and GTG): a comparative study. Hum. Genet.1990, 85, 261-266
  14. Di, Berardino. D., Ronne, M., Burguete, I., Lioi, M. B., Taibi, L. and Matassino, D. R-banding pattern of the prometaphase chromosomes of the goat. Heredity.1987, 78, 225-230