Chromosomal band pattern of black-striped field mouse (Apodemus agrarius)

한국 야생 등줄쥐의 고해상도 염색체분염상

  • Oh, Seung-hyun (Laboratory of Departmental Biology and Genomics, College of Veterinary Medicine, Seoul National University) ;
  • Yoon, Yeo-sung (Laboratory of Histology, College of Veterinary Medicine, Seoul National University) ;
  • Jin, Hee-Kyung (Laboratory of Laboratory Animal Medicine, College of Veterinary Medicine, Kyungpook National University) ;
  • Seong, Je-kyung (Laboratory of Departmental Biology and Genomics, College of Veterinary Medicine, Seoul National University)
  • 오승현 (서울대학교 수의과대학 발생.유전학교실) ;
  • 윤여성 (서울대학교 수의과대학 조직학교실) ;
  • 진희경 (경북대학교 수의과대학 실험동물학실) ;
  • 성제경 (서울대학교 수의과대학 발생.유전학교실)
  • Accepted : 2004.02.10
  • Published : 2004.06.30

Abstract

We investigated the cytogenetic characteristics of male black-striped field mouse (Apodemus agrarium) in Korea. Chromosome slides were obtained from blood cell cultures which were synchronized with thymidine blocking or not. In the chromosome slide which synchronization with thymidine blocking was employed on, the GTG(G bands by trypsin using Giemsa)-bands of high resolution were observed. The male black-striped field mouse has 48 chromosomes composed 46 autosomes and XY sex chromosomes. The centromeric regions of autosomes were positive to GTG-banding. According to this investigation, thymidine blocking in cell culture process was useful to get lengthened chromosomes. It may be necessary to employ RBG-banding technique to investigate complementary band patterns between R- and G-banding in black-striped field mouse.

Acknowledgement

Supported by : 한국과학재단

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