Properties of Enterococcus faecium isolated from the intestineof slaughting pigs

도축돈 장관에서 분리한 Enterococcus faeciurnab 특성

  • Kim, In-doc (Department of Animal Science, Dankook University) ;
  • Ahn, Mi-hyun (Department of Animal Science, Dankook University) ;
  • Seok, Ho-bong (Department of Animal Science, Dankook University)
  • 김인덕 (단국대학교 생명자원과학대학 동물자원전공) ;
  • 안미현 (단국대학교 생명자원과학대학 동물자원전공) ;
  • 석호봉 (단국대학교 생명자원과학대학 동물자원전공)
  • Accepted : 2004.11.20
  • Published : 2004.12.30


The growth characteristics and the properties of the Enterococcus faecium (E. faecium) isolated from intestine of slaughting pig were examined in order to confirm whether it will be used practically as probiotics or not. E. faecium was identified by morphological and biochemical tests including of final confirming by API strep kit. Among 264 samples, 44 isolates (16.7%) of E. faecium were observed as an exellent growth as a range of $1{\times}10^7CFU/ml{\sim}3.3{\times}10^9CFU/ml$ in viable bacterial count on MRI medium. All isolates were shown non-haemolysis on the blood agar exception of 28 isolates shown ${\alpha}$-haemolysis and were identified as E. faecium by biochemical test using API strep enzyme kits. Eight strains (18.2%) were finally selected from which they were excellent sensitivity in showing of the acid-tolerance and the bile-tolerance in compare with reference strain.


Supported by : 단국대학교


  1. 김경수, 지규만, 이상진. Streptococcus faecium의 급여가 육계의 성장과 장내 세균총 변화에 미치는 영향. 한국가금학회지. 1991, 18, 97-119
  2. 김태한. 유산균을 이용한 의약품 개발. 생물산업. 1994, 7, 28-35
  3. 박종진, 변정수, 조윤경. 동물의 장에서 분리한 Enterococcus sp.의 특성 및 분말화. 삼양제넥스 연구소. 1996, 24, 393-398
  4. 백영진. 유산균의 상업적 이용. 생물화공. 1992, 6, 21-31
  5. 백인기. 생균제의 사용 효과. 한국영양사료학회지. 1989, 13, 175-183
  6. 이진규, 김운태, 이준호. 생균제용 유산균의 분리 및 동정. 태경연구소보. 2002, 5, 429-432
  7. 한인규, 채병조, 박웅복. 돼지에 대한 Streptococcus faecium(SF-68)의 성장촉진과 해리방지효과 및 장내 미생물변화에 관한 연구. 한국축산과학연구보고. 1982, 2, 12-25
  8. Baird, D. M. Probiotics help boost feed efficiency. Feedstuffs. 1977, 49, 11
  9. Bischoff, W. E., Reynolds, T. M., Hall, G. O., Wenzel, R. P. and Edmond, M. B. Molecular epidemiology of vancomycin-resistant Entercoccus faecium in a large urban hospital over a 5-year period. J. Clinic. Microbiol. 1999, 37, 3912-3916
  10. Dilworth, B. C. and Day, E. J. Lactobacillus cultures in brooder diets. Poult. Sci. 1978, 57, 1101
  11. Fuller, R. Probiotic: Their development and use. In Probiotics of use in oppoturnistic infections. J. Inst. Microbiol. Biochem. 1995, 8, 1-7
  12. Fuller, R. Probiotics of man and animals. J. Appl. Bacteriol. 1989, 66, 365-378
  13. Kim, H. S. Characterization of Lactobacilli and Bifidobacteria as applied to dietary adjuncts. Cult. Dairy Prod. J. 1988, 23, 6-9
  14. Kobayashi, Y., Tohyama, K. and Terashima, T. Tolerance of the multiple antibiotic resistant strain, L. casei PSR 3002, to artificial digestive fluids. Jpn. J. Microbial. 1974, 29, 691-697
  15. Pollman, D. S., Danielson, D. M. and Peo, E. R. Effects of microbiol feed additives on performance of starter and growing-finishing pigs. J. Anim. Sci. 1980, 51, 557
  16. Roger, M. Faucher, M. C., Forest, P. St-Antoine, P. and Coutlee, F. Evalution of a vanA-specific PCR assay for detection of vancomycin-resistant Entercoccus faecium during a hospital outbreak. J. Clinic. Microbiol. 1999, 37, 3348-3349
  17. Salminen, S., Isolauri, E. and Salminen, E. Clinical uses of probiotics for stabilizing the gut mucosal barrier: succesful strains and future challenges. Antonie van Leeuwenhoek. 1996, 70, 347-358
  18. Schleifer, K. H. and Kilpper-Balz, R. Transfer of Streptococcus faecalis and Streptococcus faecium to the genus Enterococcus nom. rev. as Enterococcus faeccalis comb. nov. and Enterococcus faecium comb. nov. Int. J. Syst. Bacteriol. 1984, 34, 31-34
  19. Shahani, K. M. and Ayebo, A. D. Role of dietary lactobacilli in gastrointestinal microecology. Am. J. Clin. Nutr. 1980, 33, 2448-2457
  20. Sneath, P. H. A., Mair, N. S. and Sharpe, M. E. Enterococci Bergey’s manual of systematic bacteriology. pp. 1063-1065, 2nd ed. William & Wilkins. Baltimore, 1986
  21. Speck, M. L. Our industry today. J. Dairy Sci. 1975, 59, 338-343
  22. Underdahl, N. R., Torres-Medina, A. and Doster, A. Effect of Streptococcus faecium C-68 in control pigs. Am. J. Vet. Res. 1982, 43, 2227-2232
  23. Wallace, R. J. and Newbold, C. J. Probiotics for ruminants. In Probiotics: The Scientific basis. pp. 317-353. Champmann & Hall, London, 1992