Comparison of tissue tropism of Newcastle disease vaccine viruses by Immunohistochemistry techniques

면역조직화학기법을 이용한 뉴캣슬병 백신바이러스의 조직친화성 비교

  • Kim, Min-Jeong (National Veterinary Research and Quarantine Service) ;
  • Kwon, Yong-Kuk (National Veterinary Research and Quarantine Service) ;
  • Seong, Hwan-Woo (National Veterinary Research and Quarantine Service) ;
  • Kang, Shien-Young (Research Institute of Veterinary Medicine, College of Veterinary Medicine, Chungbuk National University) ;
  • Mo, In-Pil (Research Institute of Veterinary Medicine, College of Veterinary Medicine, Chungbuk National University)
  • 김민정 (국립수의과학검역원) ;
  • 권용국 (국립수의과학검역원) ;
  • 성환우 (국립수의과학검역원) ;
  • 강신영 (충북대학교 수의과대학 동물의학연구소) ;
  • 모인필 (충북대학교 수의과대학 동물의학연구소)
  • Accepted : 2004.11.27
  • Published : 2004.12.30

Abstract

Mean death time of inoculated embryonated egg is one of the methods to determine the virulence of the Newcastle disease viruses (NDV). Evaluation of tissue tropism of NDV in the host has been proposed as an another way to determine the pathogenicity of NDV based on the principal site of viral replication. To evaluate the tissue tropism among NDV, an immunohistochemistry(IHC) technique using monoclonal antibody was applied in one-day-old SPF chickens inoculated with different ND vaccine strains such as Ulster 2C, VG/GA and B1 viruses by eye drop instillation. The tissues used for this comparison were trachea, intestine, Harderian gland and cecal tonsil, which were collected at 0.5, 1, 2, 3, 5, 8, 10, 14 days post inoculation. Among test groups, chickens inoculated with B1 viurs, which is known to replicate in the respiratory system, have IHC positive staining mainly in the trachea and those inoculated with Ulster 2C have IHC positive staining mainly in the intestine. However, chickens inoculated with VG/GA strain have IHC positive staining in both the trachea and intestine. Therefore, a differences in tissue tropism among ND vaccine strains has been proved by the IHC technique. Based on this results, the IHC staining technique could be used to classify the NDV or to study the pathogenesis of NDV in chickens.

Acknowledgement

Supported by : 충북대학교

References

  1. Alexander, D. J. Newcastle disease and other avian Paramyxoviridae infections. Disease of Poultry. pp. 541-569, 10th ed. Iowa State University Press, Iowa, 1997
  2. Doyle, T. M. A hitherto unrecorded disease of fowls due to a filterpassing virus. J. Comp. Pathol. Therap. 1927, 40, 144-169
  3. Hamid, H., Campbell, R. S. F. and Lamichhane, C. M. The pathology of infection of chickens with the lentogenic V4 strain of Newcastle disease virus. Avian Pathol. 1990, 19, 687-696
  4. Hansson, E., Young, J. G., Hooper, P. T. and Della-Porta, A. J. Virulence and transmissibility of some Australian and exotic strains of Newcastle disease virus used in some vaccines. Aust. Vet. J. 1999, 77, 51-52
  5. Holmes, H. C. Resistance of the respiratory tract of the chicken to Newcastle disease virus infection following vaccination: The effect of passively acquired antibody on its development. J. Comp. Pathol. 1979, 89, 11-20
  6. Russell, P. H. Newcastle disease virus: Virus replication in the Harderian gland stimulates lacrimal IgA; the yolk sac provides early lacrimal IgG. Vet. Immunol. Immunopathol. 1993, 37, 151-163
  7. Sally, J. N. Handbook of immunohistochemical staining methods. 17th ed. DAKO Co. 1989
  8. Spanoghe, L., Peeters, J. E., Cotlear, J. C., Devos, A. H. and Viaene, N. Kinetics of serum and local hemagglutination inhibition antibodies in chicks following vaccination and experimental infection with Newcastle disease virus and their relation with immunity. Avian Pathol. 1997, 6, 101-109
  9. Lockaby, S. B., Hoerr, F. J., Ellis, A. C. and Yu, M. S. Immunohistochemical detection of Newcastle disease virus in chickens. Avian Dis. 1993, 37, 433-437
  10. Abdul-Aziz, T. A. and Arp, L. H. Pathology of the trachea in turkeys exposed by aerosol to lentogenic strains of Newcastle disease virus. Avian Dis. 1983, 27, 1002-1011
  11. Jonsson, L. G. O. and Engstrom, B. E. Immunohistochemical detection of infectious bursal disease and infectious bronchitis viral antigens in fixed, paraffin-embedded chicken tissues. Avian Pathol. 1986, 15, 385-393
  12. Kim, S. J., Spradbrow, P. B. and Chung, Y. S. The serological response of chickens to Australian lentogenic strains of Newcastle disease virus. Aust. Vet. J. 1978, 54, 430-436
  13. Russell, P. H. Newcastle disease virus vaccines: differences between Line C and Line 151 chickens with respect to virus replication and IgA responses in the gut and Harderian gland. Vet. Immunol. Immunopathol. 1994, 42, 357-365
  14. Hamid, H., Campbell, R. S. F., Lamihhance, C. M. and Graydon, R. Indirect immunoperoxidase staining for Newcastle disease virus (NDV). In Proceedings. 2nd Asian/Pacific Poulty Health Conf. Aust. Vet. Poult. Assoc. 1988, 425-427
  15. Gough, R. E. and Allan, W. H. The potential as an aerosol vaccine of Ulster 2C strain, Newcastle disease virus. Vet. Rec. 1974, 95, 263-265
  16. Borland, L. J. and Allan, W. H. Laboratory tests for comparing live lentogenic Newcastle disease vaccines. Avian Pathol. 1980, 9, 45-49
  17. Cheville, N. F., Stone, H., Riley, J. and Ritchie, A. E. Pathogenesis of virulent Newcastle disease in chickens. J. Am. Vet. Med. Assoc. 1972, 161, 169-179
  18. Suzan, H. M., Claassen, E., Boonstra-Blom, A. G., Vervelde, L. and Janse, E. M. Immunocytochemical techniques to investigate the pathogenesis of infectious micro-organism and the concurrent immune response of the host. Develop. Comp. Immunol. 2000, 24, 141-21
  19. Thijs, K., Wobeser, G., Leighton, F. A., Haines, D. M., Chelack, B., Bogdan, J., Hassard, L., Heckert, R. A. and Riva, J. Pathology of Newcastle disease in double-crested cormorants from Saskatchewan with comparison of diagnostic methods. J. Wildl. Dis. 1999, 35, 8-23
  20. Glickman, R. L. and Syddall, R. T. Quantitative basis residue re quirements in the cleavage-activation site of the fusion glycoprotein as determinant of virulence for Newcastle disease virus. J. Virol. 1988, 62, 354-356
  21. Rott, R. Molecular basis of infectivity and pathogenicity of myxoviruses. Arch. Virol. 1979, 59, 285-298
  22. Alexander, D. J. Characteristic of the VG/GA strain. Avian Pathol. 1994, 24, 3-10
  23. Timms, L. and Alexander, D. J. Cell-mediated immune response of chickens to Newcastle disease vaccines. Avian Pathol. 1977, 6, 51-59
  24. Beard, C. W. and Wikes, W. J. A. Simple and rapid microtest procedure for determining Newcastle hemagglutination-inhibition (HI) antibody titer, in Proceedings, 77th Annual Meeting of the US Animal Health Association, 1973, 596-600
  25. Hooper, P. T., Russell, G. M., Morrow, C. J. and Sehal, Y. Lentogenic Newcastle disease virus and respiratory disease in Australian broiler chickens. Aust. Vet. J. 1999, 77, 17-18
  26. Kotani, T., Odagiri, Y., Nakamura, J. and Horiuchi, T. Pathological change of mucosa in chickens infected with lentogenic Newcastle disease virus. Avian Dis. 1987, 31, 491-497
  27. Brown, C. C., King, D. J. and Seal, B. S. Pathogenesis of Newcastle disease in chickens experimentally infected with viruses of different virulence. Vet. Pathol. 1999, 36, 125-132
  28. Alexander, D. J., Mackenzie, R. J. and Russell, P. H. Use of monoclonal antibodies in the characterization of avian paramyxovirus type 1(Newcastle disease virus) isolates submitted to an international reference laboratory. Avian Pathol. 1987, 16, 553-565
  29. Bruce, S. S., Daniel, J. K. and Holly, S. S. The avian response to Newcastle disease virus. Develop. Comp. Immunol. 2000, 24, 257-268
  30. Kraneveld, F. C. A poultry disease in the Dutch East Indies. Ned. Indisch. B1 Diergeneeskd. 1926, 38, 448-450
  31. Beard, C. W., Villegas, P. and Glisson, R. Comparative efficacy of the B-1 and VG/GA vaccine strains against velogenic viscerotropic Newcastle disease virus in chickens. 1993, Avian Dis. 37, 222-225
  32. Frederick, A. M., Paul, J. G., Marian, C. H. and Michael, J. S. Veterinary Virology. pp. 97-100, 2nd ed. Academic press, San Diego, 1999
  33. Gough, R. E. and Alexander, D. J. The speed of resistance to challenge induced in chickens vaccinated by different routes with a B1 strain of live NDV. Vet. Rec. 1973, 92, 563-564
  34. Brown, C. C., King, D. J. and Seal, B. S. Comparison of pathology based techniques for detection of viscerotropic velogenic Newcastle disease virus on chickens. J. Comp. Pathol. 1999, 120, 383-389
  35. Russell, P. H. and Koch, G. Local antibody forming cell responses to the Hitchner B1 and Ulster strains of Newcastle disease virus. Vet. Immunol. Immunolpathol. 1993, 37, 165-180
  36. Nagai, Y., Klenk, H. D. and Rott, R. Proteolytic cleavage of the viral glycoproteins and its significance for the virulence of NDV. Virology. 1976, 72, 494-508
  37. Ojok, L. and Brown, C. C. An immunohistochemical study of the pathogenesis of virulent viscerotropic Newcastle disease in chickens. J. Comp. Pathol. 1996, 115, 221-227