Analysis of gamma-ray-induced DNA damage in human, mouse and rat peripheral blood lymphocytes using single-cell gel electrophoresis

단세포 전기영동법을 이용한 인체, 마우스 및 랫드 림프구의 방사선에 의해 유발된 DNA 손상 측정

  • Oh, Heon (Radiation Food Technology and Bioscience Team, Korea Atomic Energy Research Institute) ;
  • Jung, Uhee (Radiation Food Technology and Bioscience Team, Korea Atomic Energy Research Institute) ;
  • Park, Hae-Ran (Radiation Food Technology and Bioscience Team, Korea Atomic Energy Research Institute) ;
  • Kim, Sung-Ho (College of Veterinary Medicine, Chonnam National University) ;
  • Jo, Sung-Kee (Radiation Food Technology and Bioscience Team, Korea Atomic Energy Research Institute)
  • 오헌 (한국원자력연구소 방사선식품.생명공학팀) ;
  • 정우희 (한국원자력연구소 방사선식품.생명공학팀) ;
  • 박혜란 (한국원자력연구소 방사선식품.생명공학팀) ;
  • 김성호 (전남대학교 수의과대학) ;
  • 조성기 (한국원자력연구소 방사선식품.생명공학팀)
  • Accepted : 2004.02.25
  • Published : 2004.03.31

Abstract

The alkaline single-cell gel electrophoresis (SCGE) assay, called the comet assay, has been applied to detect DNA damage induced by a number of chemicals and biological factors in vivo and in vitro. The DNA damage was analysed by tail moment (TM) and tail length (TL), which were markers of DNA strand breaks in SCGE. Human, mouse and rat peripheral blood lymphocytes (PBLs) were irradiated with different doses of $^{60}Co$ ${\gamma}$-rays, e.g. 1, 2, 4, and 8 Gy at a dose rate of 1 Gy/min. A dose-dependent increase in TM (p<0.01) and TL (p<0.01) was obtained at all the radiation doses (1-8 Gy) in human, mouse and rat PBLs. Mouse PBLs were more sensitive than human PBLs which were in turn more sensitive than rat PBLs when the treated dosages were 1 and 2 Gy. However, human PBLs were more sensitive than mouse PBLs which were in turn more sensitive than rat PBLs when the irradiation dosages were 4 and 8 Gy. Data from all three species could be fitted to a linear-quadratic model. These results indicated that there may be inherent differences in the radio-sensitivity among PBLs of mammalian species.

Acknowledgement

Supported by : 과학기술부

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