Prevalence of isolated microorganisms and antimicrobial susceptibility from half milk in dairy goats

젖염소 분방 유즙에서 분리한 세균 및 항균제 감수성 조사

  • Yoon, Joon-chul (College of Veterinary Medicine, Chonnam National University) ;
  • Lee, Jeong-chi (Department of Veterinary Nurse and Pets Sciences, Seojeong College) ;
  • Kim, Sang-ki (College of Veterinary Medicine, Chonnam National University) ;
  • Park, Young-seok (Department of Companion and Laboratory Animal Science, Kongju National University) ;
  • Kim, Jong-taek (Department of Veterinary Medicine, Kangwon National University) ;
  • Lee, Chung-gil (College of Veterinary Medicine, Chonnam National University) ;
  • Lee, Chai-yong (College of Veterinary Medicine, Chonnam National University)
  • 윤준철 (전남대학교 수의과대학) ;
  • 이정치 (서정대학 애완동물과) ;
  • 김상기 (전남대학교 수의과대학) ;
  • 박영석 (공주대학교 특수동물학과) ;
  • 김종택 (강원대학교 수의학과) ;
  • 이정길 (전남대학교 수의과대학) ;
  • 이채용 (전남대학교 수의과대학)
  • Accepted : 2004.03.05
  • Published : 2004.03.31

Abstract

Samples of milk were collected from 425 halves of 216 dairy goats in Chonnam province over a period of January through August 2003. Bacterial isolation was carried out on those samples, and their antimicrobial susceptibility was tested. Bacteria were isolated from 166 milk samples (39.1%), either singly (74.7%) or in combination (25.3%). Of the 220 isolates, Staphylococcus spp. was the most prevalent (82.6%), followed by Streptococcus spp. (2.7%), Corynebacterium spp. (1.8%), Enterococcus spp. (1.8%), and Pasteurella spp. (1.8%). Of the 11 species identified from the 182 isolates of Staphylococcus spp., the most frequent species identified were S. epidermidis (28.6%) followed by S. chromogens (14.8%), S. haemolyticus (12.6%), S. aureus (12.1%), S. capitis (8.2%), S. lentus (8.2%), S. hyicus (4.4%), S. simulans (4.4%), S. caprae (2.8%), S. hominis (2.8%) and S. warneri (1.1%). Antimicrobial sensitivity test revealed that most isolates were highly susceptible to 11 antimicrobial agents (96.4 ~ 80.9%), while most isolates were resistant to penicillin.

References

  1. 김태진, 나광연, 김종우. 산양유의 품질보존에 관한 연구. 한국축산학회지. 1970, 12, 214-220
  2. 이정치, 이채용, 김상기, 이정길, 서국현. Holstein 유우의 유즙에서 분리한 유방염 원인균의 항균제 감수성. 한국임상수의학회지. 2003, 20, 166-171
  3. 함준상, 인영민, 정석근, 김동운, 김희발, 김용곤, 안영태, 김현욱. 산양유 쿠미스의 제조와 Candida kefir의 젖산 생성. 한국유가공기술과학회지. 2000, 18, 151-163
  4. 임동현, 전우민, 한경식, 김병철, 황광연, 김세현. DEAE-Cellulose에 의한 산양유 casein의 분별 및 이화학적 성질에 관한 연구. 한국유가공기술과학회지. 2000, 18, 1-8
  5. 정길택. 산양유의 앨콜침전시험에 관한 연구. 대한수의학회지. 1964, 4, 19-22
  6. 통계청. http://www.nso.go.kr:7001/kosis/winkosis.htm
  7. Ahmad, M., Al-Majali and Jawabreh, S. Period prevalence and etiology of subclinical mastitis in Awassi sheep in southern Jordan. Small Ruminant Res.2003, 47, 243-248
  8. Albenzio, M., Taibi, L., Muscio, A. and Sevi, A. Prevalence and etiology of subclinical mastitis in intensively managed flocks and related changes in theyield and quality of ewe milk. Small Ruminant Res. 2002, 43, 219-226
  9. Bauer, A. W., Kirby, W. M., Sherris, T. C. and Turck, M. Antibiotic susceptibility testing by a standardized single disk method. Am. J. Clin. pathol. 1966, 45, 493-496
  10. Contreras, A., Corrales, J. C., Sanchez, A. and Sierra, D. Persistence of subclinical intramammary pathogens in goats throughout lactation. J. Dairy Sci.1997, 80, 2815-2819
  11. Contreras, A., Corrales, J. C., Sierra, D. and Marco, J. Prevalence and aetiology of non-clinical intramammary infection in Murciano - Granadina goats. Small Ruminant Res. 1995, 17, 71-78
  12. Contreras, A., Luengo, C., Sanchez, A. and Corrales, J. C. The role of intramammary pathogens in dairy goats. Livestock Prod. Sci. 2003, 79, 273-283
  13. Contreras, A., Paape, M. J. and Miller, R. H. Prevalence of subclinical intramammary infection caused by Staphylococcus epidermidis in a commercialdairy goat herd. Small Ruminant Res. 1999, 31, 203-208
  14. Contreras, A., Sierra, D., Corrales, J. C., Sanchez, A. and Marco, J. Physiological threshold of somatic cell count and California mastitis test for diagnosis of caprine subclinical mastitis. Small Ruminant Res. 1996, 21, 259-264
  15. Das, M. and Singh, M. Variation in blood leucocytes, somatic cell count, yield and composition of milk of crossbred goats. Small Ruminant Res. 2000, 35, 169-174
  16. Deinhoger, M. and Pernthaner, A. Staphylococcus spp. as mastitis-related pathogens in goat milk. Vet. Microbiol. 1995, 43, 161-166
  17. Droke, E. A., Paape, M. J. and Di Carlo, A. L. Prevalence of high somatic cell counts in bulk tank goat milk. J. Dairy Sci. 1993, 76, 1035-1039
  18. Dulin, A. M., Paape, M. J., Schultz, W. D. and Weinland, B. T. Effect of parity, stage of lactation, and intramammary infection on concentration of somatic cells and cytoplasmic particles in goat milk. J. Dairy Sci. 1983, 66, 2426-2433
  19. East, N. E., Birnie, E. F. and Farver, T. B. Risk factors associated with mastitis in dairy goats. Am. J. Vet. Res. 1987, 48, 776-779
  20. FAOSTAT. http://apps.fao.org/cgi-bin/nph-db.pl
  21. Haenlein, G. F. W. Relationship of somatic cell counts in goat milk to mastitis and productivity. Small Ruminant Res. 2002, 45, 163-178
  22. Hunter, A. C. Microflora and somatic cell content of goat milk. Vet. Rec. 1984, 114, 318-320
  23. Kirk, J. H., Glenn, J. S. and Maas, J. P. Mastitis in a flock of milking sheep. Small Ruminant Res. 1996, 22, 187-191
  24. Lewter, M. M., Mullowney, P. C., Baldwin, E. W. and Walker, R. D. Mastitis in goats. Compend. Contin. Educ. Pract. Vet. 1984, 6, 417-425
  25. Manser, P. A. Prevalence, causes, and laboratory diagnosis of subclinical mastitis in the goat. Vet. Rec. 1986, 118, 552-554
  26. McDougall, S., Murdough, P., Pankey, W., Delaney, C., Barlow, J. and Scruton, D. Relationships among somatic cell count, California mastitis test, impedanceand bacteriological status of milk in goats and sheep in early lactation. Small Ruminant Res. 2001, 40, 245-254
  27. McDougall, S., Pankey, W., Delaney, C., Barlow, J., Murdough, P. A. and Scruton, D. Prevalence and incidence of subclinical mastitis in goats and dairy ewes in Bermont, USA. Small Ruminant Res. 2002, 46, 115-121
  28. Perrin. G. G., Mallereau, M. P., Lenfant, D. and Baudry, C. Relationships between California mastitis test (CMT) and somatic cell counts in dairy goats.Small Ruminant Res. 1997, 26, 167-170
  29. Poutrel, B. and Lerondelle, C. Cell content of goat milk: California mastitis test, Coulter counter, and Fossomatic for predicting half infection. J. Dairy Sci. 1983, 66, 2575-2579
  30. Poutrel, B., de Cremoux, R., Ducelliez, M. and Verneau, D. Control of intramammary infections in goats: Impact on somatic cell counts. J. Anim. Sci.1997, 75, 566-570
  31. Ryan, D. P. and Greenwood, P. L. Prevalence of udder bacteria in milk samples from four dairy goat herds. Aust. Vet. J. 1990, 67, 362-363
  32. Smith, M. C. and Roguinsky, M. Mastitis and other diseases of the goat’s udder. J. Am. Vet. Med. Assoc. 1977, 171, 1241-1248
  33. Sung, Y. Y., Wu, T. I. and Wang, P. H. Evaluation of milk quality of Alpine, Nubian, Saanen and Toggenburg breeds in Taiwan. Small Ruminant Res. 1999, 33, 17-23
  34. White, E. C. and Hinckley, L. S. Prevalence of mastitis pathogens in goat milk. Small Ruminant Res. 1999, 33, 117-121
  35. Wilson, D. J., Stewart, K. N. and Sears, P. M. Effects of stage of lactation, production, parity and season on somatic cell counts in infected and uninfected dairy goats. Small Ruminant Res. 1995, 16, 165-169
  36. Zeng, S. S. Comparison of goat milk standards with cow milk standards for analyses of somatic cell count, fat and protein in goat milk. Small Ruminant Res. 1996, 21, 221-225
  37. Zeng, S. S. and Escobar, E. N. Effect of parity and milk production on somatic cell count, standard plate count and composition of goat milk. Small RuminantRes. 1995, 17, 269-274
  38. Zeng, S. S., Escobar, E. N. and Popham, T. Daily variations in somatic cell count, composition, and production of Alpine goat milk. Small Ruminant Res. 1997, 26, 253-260
  39. Zeng, S. S., Escobar, E. N., Hart, S. P. Hinckley, L., Baulthaus, M., Robinson, G. T. and Jahnke, G. Comparative study of the effects of testing laboratory,computing method, storage and shipment on somatic cell counts in goat milk. Small Ruminant Res. 1999, 31, 103-107