Efficacy of Ciprofloxacin for the Control of Streptococcal Infection in Cultured Fish, Flounder(Paralichtyhs olivaceus) and Eel(Anguilla japonica)

넙치(Paralichthys olivaceus) 및 뱀장어(Anguilla japonica)의 연쇄구균즈에 대한 시프로플로삭신의 치료 효과

  • Park, Se Chang (Collage of veterinary medicine, Seoul National University) ;
  • Heo, Gang-Joon (Collage of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National university)
  • 박세창 (서울대학교 수의과대학) ;
  • 허강준 (충북대학교 수의과대학 및 동물의학연구소)
  • Accepted : 2004.11.08
  • Published : 2004.12.30

Abstract

Efficacy of ciprofloxacin was evaluated in laboratory and field studies for control of streptococcal infection in flounder and eel. In disc diffussion test, all streptococcal strains showed high sensitivity to the ciprofloxacin. Minimum inhibitory concentrations of ciprofloxacin against all streptococcal strains used in this study were less than $0.195{\mu}g/ml/$. In laboratory studies where fishes were challanged with Streptococcus iniae, significant reductions in mortality were observed among fish receiving ciprofloxacin (in fed) at 100 mg/kg/body weight or more daily for 3 d when compared with mortality of non-medicated controls. Similar results were achieved when 100 mg/kg/body weight of ciprofloxacin was used in field trials.

Acknowledgement

Supported by : 충북대학교

References

  1. Aoki, T., Kitao, T. and Fukudome, M. Chemotherapy against infection with multiple drug resistant strains of Edwardsiella tarda in cultured eels. Fish Pathol. 1989, 24, 161-168
  2. Dodson, S. V., Maurer, J. J. and Shotts, E. B. Biochemical and molecular typing of Streptococcus iniae isolated from fish and human cases. J. Fish. Dis. 1999, 22, 331-336
  3. Endo, T., Ogishima. K., Hayasaka. H., Kaneko, S. and Ooshima, S. Oxolinic acid as a therapeutic agent against infection in fish-1. Its antibacterial activity,therapeutic effect and excretion in fish body. Nippon Suisan Gakkaishi. 1973, 39, 165-171
  4. Grimm, H. Interpretive criteria for the agar diffusion susceptibility test with norfloxacin. Drugs. 1987, 34, 14-19
  5. Heo, G. J. and Kim, J. H. A study on efficacy and safety of antibacterial (pefloxacin methanesulfonate) to cultured fish, Cyprinus caprio and Paralichthysolivaceus. Korean J. Vet. Res. 1994, 34, 153-161
  6. Heo, G. J., Shin, G. S. and Lee, M. H. Disease of aquaculture animals and prevention of drug residues. Korean J. Food Hygiene. 1992, 7, 7-14
  7. Kaatz, G. W., Barrier, S. L., Schaberg, D. R. and Fekery, R. The emergence of resistance to ciprofloxacin during treatment of experimental Staphylococcus aureus endocarditis. J. Antimicrob. Chemother. 1987, 20, 753-758
  8. Kaatz, G. W., Seo, S. M., Barrier, S. L., Albrecht, L. M. and Rybak, M. J. Ciprofloxacin and rifampin, alone and in combination, for therapy of experimentalStaphylococcus aureus endocarditis. Antimicrob. Agents Chemother. 1989, 33, 1184-1189
  9. Kusuda, R., Kawai, K., Salati, F., Banner, C. R. and Fryer, J. L. Enterococcus seriolicida sp. nov. a new fish pathogen. Int. J. Syst. Bacteriol. 1991, 41, 406-409
  10. Kusuda, R., Kawai, K., Toyoshima, T. and Komatsu, I. A new pathogenic bacterium belonging to the genus Streptococcus, isolated from an epizootic of cultured yellowtail. Bull. Jpn. Soc. Scient. Fish. 1976, 42, 1345-1352
  11. Lee, H. K. Isolation and antimicrobial susceptibility testing of Edwarsiella tarda from Channa argus in Korea. Bull. Korean Soc. Fish Pathol. 1988, 1, 95-102
  12. Markwardt, M. N. and Klontz, G. W. A method to eliminate the symptomatic carrier state of Aeromonas salmonicida in salmonids. J. Fish Dis. 1989, 12, 317-322
  13. McCracken, A., Fidgeon, S., O’Brien, J. J. and Anderson, D. An investigation of antibiotic and drug residues in fish. J. Appl. Bacteriol. 1992, 40, 61-65
  14. Monk, J. P. and Campoli-Richards, D. M. Ofloxacin review of its antibacterial activity, pharmokinetic properties and therapeutic use. Drugs. 1987, 33, 346-351
  15. Nakano, S., Aoki, T. and Kitao, T. In vitro antimicrobial activity of pyridonecarboxylic acids against fish pathogens. J. Aquat. Anim. Health. 1989,1, 43-50
  16. Roberts, R. J. Fish Pathology, p. 324-326, 3rd ed. Saunders, Philadelphia, 2001
  17. Robillard, N. J. and Scarpa, A. L. Genetic and physiological characterization of ciprofloxacin resistance in Pseudomonas aeruginosa PAO. Antimicrob. AgentsChemother. 1988, 32, 535-541
  18. Roy, P., Kawai, K. and Kusuda, R. In vitro activity of quinolone antibacterials against selected fish pathogens. Fish Pathol. 1992, 27, 131-138
  19. Smith, J. T. and Lewin, S. Chemistry and mechanisms of action of the quinolone antibacterials. p. 23. In Andriole, V. T. (ed.), The quinolones, Academic Press, New York. 1988
  20. Stamm, J. M. In vitro resistance by fish pathogens to aquacultural antobacterials, including the quinolones difloxacin(A-56619) and sarafloxacin(A-51620). J. Aquat. Anim. Health. 1989, 1, 135-141
  21. Tanasomwang, M. and Muroga, M. Effects of tetracycline and sodium nifurstyrenate and tetracycline on the bacterial flora of rotifer (Brachionus plicatilis). Fish Pathol. 1989, 24, 29-35
  22. Tsoumas, A., Alderman, D. J. and Rodgers. C. J. Aeromonas salmonicida; development of resistance to 4-quinolone antimicrobials. J. Fish Dis. 1989, 12, 493-499
  23. 本化學療法學會. 最小發育沮上濃度 (MIC)測定法再改訂について. Chemotherapy. 1981, 29, 76-79
  24. 家畜 耐性菌硏究會. 家畜由來の細菌に對する抗生物質等の藥劑の最小發育抵上 濃度測定法について. 日本獸醫學會誌. 1976, 29, 90-92