Study on the quantitation of moxidectin by fluoroscence derivatization and it's residual after subcutaneously injection in pigs

형광유도체화법을 이용한 Moxidectin 정량 및 피하주사 후 돼지에서의 잔류 연구

  • Jang, Beom-su (College of Veterinary Medicine, Chungnam National University) ;
  • Lim, Jong-hwan (College of Veterinary Medicine, Chungnam National University) ;
  • Park, Byung-kwon (College of Veterinary Medicine, Chungnam National University) ;
  • Kim, Min-Kyu (College of Veterinary Medicine, Seoul National University) ;
  • Yun, Hyo-in (College of Veterinary Medicine, Chungnam National University)
  • 장범수 (충남대학교 수의과대학) ;
  • 임종환 (충남대학교 수의과대학) ;
  • 박병권 (충남대학교 수의과대학) ;
  • 김민규 (서울대학교 수의과대학) ;
  • 윤효인 (충남대학교 수의과대학)
  • Accepted : 2004.02.23
  • Published : 2004.03.31

Abstract

We established a new method to analyze moxidectin using high performance liquid chromatography(HPLC) with fluorescence derivatization in order to obtain its residual profiles in biological samples. Recovery of moxidectin in tissue was 62% at 10 ppb. Average detection reproducibility in terms of coefficience variation was 4.47% at 0.32 to 10 ppb. Residual of moxidectin was studied in 44 Yorkshire-Landrace mixed bred male pigs administered subcutaneously 0, 200, or $800{\mu}g/kg$ body weight (BW) Residual profiles of moxdectin in blood, muscle, liver, kidney and fat of pigs were described. The concentration of the moxidectin in liver after administration of moxidectin was the highest among the tissues examined. Moxidectin in liver after administration of moxidectin as $200{\mu}g/kg$ BW was declined from $10.0{\pm}3.7ng/g$ at 10 day post administration to $0.5{\pm}0.3ng/g$ level at 40 day post administration. Residual levels of moxidectin in all samples were estimated to fall below the limit of quantitation (0.32 ng/ml) after 50 day after treatment of $200{\mu}g/kg$. Moxidectin showed no abnormal observations in all the clinical findings at any concentrations under these experimental conditions. In conclusion, this analysis method by HPLC after fluorescence derivatization was very effective for the detection of moxidectin in biological samples. We suggest that 50-day is safe enough for the withdrawal time of moxidectin in pigs, following the recommendation dose by the manufacturer.

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