Identification of porcine intestinal spirochetes isolated from Korea by NADH oxidase gene(nox) PCR-RFLP

NADH oxidase(nox) 유선자의 PCR- RFLP를 이용한 돼지 intestinal spirochetes 국내분리주의 동정

  • Kim, Tae-Jung (College of Veterinary Medicine, Chonnam National University) ;
  • Kim, Myoung-Hee (College of Veterinary Medicine, Chonnam National University) ;
  • Na, Young-Ran (College of Veterinary Medicine, Chonnam National University) ;
  • Jung, Suk-Chan (National Veterinary Research and Quarantine Service) ;
  • Lee, Jae-Il (College of Veterinary Medicine, Chonnam National University)
  • 김태중 (전남대학교 수의과대학) ;
  • 김명희 (전남대학교 수의과대학) ;
  • 나영란 (전남대학교 수의과대학) ;
  • 정석찬 (국립수의과학검역원) ;
  • 이재일 (전남대학교 수의과대학)
  • Accepted : 2004.10.22
  • Published : 2004.12.30

Abstract

In this study, we performed a PCR-RFLP analysis of NADH oxidase gene(nox) for the characterization of porcine intestinal spirochetes isolated from Korea by the comparison with Brachyspira hyodysenteriae and B. pilosicoli reference strains. Eleven strains including four reference strains, B. hyodysenteriae B204, B234, B169, B. pilosicoli P43/6/78 and seven Korean isolates were used. PCR products of 939 bp were amplified using nox-specific primers and digested with two restriction enzymes, Bfm I and Dpn II. In study using Bfm I, both strains showed no difference in fragmented size(197 and 741 bp). When use Dpn II, B. hyodysenteriae showed two bands(209 and 684 bp), however B. pilosicoli showed a single band of 896 bp. Our results indicate that nox-specific PCR-RFLP could be used as a typing method of Brachyspira species and as an epidemiological method for identifying spirochetes isolated from swine.

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