- Volume 17 Issue 7
PSMC5 subunit, which belongs to the 26S proteasomal subunit family, plays an important role in the antigen presentation mediated by MHC class I molecular. Full-length cDNA of porcine PSMC5 was isolated using the in silico cloning and rapid amplification of cDNA ends (RACE). Amino acid was deduced and the primary structure was analyzed. Results revealed that the porcine PSMC5 gene shares the high degree of sequence similarity with its mammalian counterparts at both the nucleotide level and the amino acid level. The RT-PCR was performed to detect the porcine PSMC5 expression pattern in seven tissues and the result showed that high express level was observed in spleen, lung, marrow and liver while the low express level was in muscle. The full-length genomic DNA sequence of porcine PSMC5 gene was amplified by PCR and the genomic structure revealed that this gene was comprised by 12 exons and 11 introns. Best alignment of the cDNA and genomic exon DNA sequence presents 4 mismatches and this information potentially bears further study in gene polymorphisms.
Sequences Analysis;Expression Profiles;Porcine;PSMC5
- Tsukamoto, T., S. Miura, T. Nakai, S. Yokota, N. Shimozawa, Y. Suzuki, T. Orii, Y. Fujiki, F. Sakai, A. Bogaki, H. Yasumo and T. Osumi. 1995. Peroxisome assemble factor-2, a putative ATPase cloned by functional complementation on a peroxisome-deficient mammalian cell mutant. Nat. Genet. 11:395-401.
- Hoyle, J., K. H. Tan and E. M. C. Fisher. 1997. Localization of genes encoding two human one-domain members of the AAA family: PSMC5 (the thyroid receptor-interacting protein, TRIP1) and PSMC3 (the tat-binding protein, TBP1). Hum. Genet. 99:285-288.
- Rubin, D. M., M. H. Glickman, C. N. Larsen, S. Dhruvakumar and D. Finley. 1998. Active site mutants in the six regulatory particle ATPases reveal multiple roles for ATP in the proteasome. EMBO. J. 17:4909-4919.
- Thompson, J. D., D. G. Higgins and T. J. Gibson. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic. Acids. Res. 22:4673-4680.
Walker, J. E., M. Saraste, M. J. Runswick and N. J. Gay. 1982. Distantly related sequences in the
$\alpha$- and $\beta$-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold. EMBO. J. 1:945-951.
- Tanahashi, N., M. Suruki, T. Fujiwara, E. Takahashi, N. Shimbara, C. H. Chung and K. Tanaka. 1998. Chromosomal localization and immunological analysis of a family of human 26S proteasomal ATPase. Biochem. Biophys. Res. Commun. 243:229-232.
- Pan, P W., S. H. Zhao, M. Yu, B. Liu, T. A. Xiong and K. Li. 2003. Identification of differentially expressed genes in the longissimus dorsi muscle tissue between duroc and erhualian pigs by mRNA differential display. Asian-Aust. J. Anim. Sci. 16:1066-1070.
- Li, Y. H., J. Chambers, J. Pang, K. Ngo, P. A. Peterson, W. P. Leung and Y. Yang. 1999. Characterization of the mouse proteasome regulator PA28b gene. Immunogenetics 49:149-157.
- Park, B. W., D. M. O’Rourke, Q. Wang, J. G. Davis, A. Post, X. L. Qian and M. I. Greene. 1999. Induction of the tat-binding protein 1 gene accompanies the disabling of oncogenic erbB receptor tyrosine kinases. Proc. Natl. Acad. Sci. 96:6434-6438.
- Makino, Y., S. Yogosawa, M. Kanemaki, T. Yoshida, K. Tamano, T. Kishimoto, V. Moncollin, J. M. Egly, M. Muramatsu and T. Tamura. 1996. Structures of the rat proteasomal ATPases:determination of highly conserved structural motifs and rules for their spacing. Biochem. Biophys. Res. Commun. 220:1049-1054.
- Lee, J. W., H. S. Choi, J. Gyuris, R. Brent and D. D. Moore. 1995. Two classes of proteins dependent on either the presence or absence of thyroid hormone for interaction with the thyroid hormone receptor. Molec. Endocr. 9:243-254.
- DeMartino, G. N., C. R. Moomaw, O. P. Zagnitko, R. J. Proske, M. Chu-Ping, S. J. Afendis, J. C. Swaffield and C. A. Slaughter. 1994. PA700, an ATP-dependent activator of the 20S proteasome, is an ATPase containing multiple members of a nucleotide-binding protein family. J. Biol. Chem. 269:20878-20884.
- Liu, B., G. L. Jin, S. H. Zhao, M. Yu, T. A. Xiong, Z. Z. Peng and K. Li. 2002. Preparation and analysis of spermatocyte meiotic pachytene bivalents of pigs for gene mapping. Cell. Res. 12(5-6):401-405.
- Hershko, A. and A. Ciechanover. 1998. The ubiquitin system. Annu. Rev. Biochem. 67:425-479.
- Ma, C. P., C. A. Slaughter and G. N. Demartino. 1992. Identification, purification and characterization of a protein activator (PA28) of the 20S proteasome Macropain). J. Biol. Chem. 267:10515-10523.
- Ma, C. P., J. H. Vo, R. J. Proske, C. A. Slaughter and G. N. Demartino. 1994. Identification, purification, and characterization of a high-molecular weight, ATP-dependent activator (PA700) of the 20S proteasome. J. Biol. Chem. 269:3539-3547.
- Wang, Y. F., M. Yu, M. Yerle, B. Liu, S. H. Zhao, T. A. Xiong, B. Fan and K. Li. 2003. Mapping of genes encoding four ATPase and three non-ATPase components of the pig 26S proteasome. Anim. Genet. 34:393-395.
- Yu, M., S. H. Zhao, B. Liu, T. A. Xiong, P. W. Pan and K. Li. 2001. Isolation and regional localization of the porcine glial fibrillary acidic protein (GFAP) gene. J. Anim. Sci. 79:2754.
- 1. Molecular characterization, expression analysis and association study with immune traits of porcine PSMB6 gene vol.38, pp.8, 2011, doi:10.5713/ajas.2004.897