Mutagenicity Studies of the Herbicide-resistance Phosphinotricin Acetyltransferase (PAT)

제초제저항성단백질 Phosphinotricin Acetyltransferase (PAT)의 유전독성시험

  • 정미혜 (농업과학기술원 유해물질과) ;
  • 유아선 (농업과학기술원 유해물질과) ;
  • 이제봉 (농업과학기술원 농약평가과) ;
  • 신진섭 (농업과학기술원 농약평가과) ;
  • 김진화 (농업과학기술원 유해물질과) ;
  • 한증술 (원예연구소 원예생명공학과)
  • Published : 2004.03.30


To evaluate mutagenicity of Phosphinotricin Acetyltransferase(PAT) which is expressed by the glufosinate-resistance gene pat, in vitro reverse mutation test using Salmonella typhimurium, chromosome aberration test using chinese hamster lung(CHL) cells and in vivo micronucleus test of mice were performed. In the reverse mutation, the PAT did not induce mutagenicity in Salmonella typhimurium TA 98, TA 100, TA 1535, TA 1537 with and without metabolic activation at $5000{\mu}g/plate$. In the chromosome aberration test, the results showed no incidence of increased structural and numerical chromosome aberrations at any doses tested(100, 10, $1{\mu}g/mL$). In micronucleus test, the ratio of micronuclei was measured in polychromatic erythrocytes of bone marrow of male ICR mice intraperitoneally administrated with PAT(1250, 625, and 313 mg/kg), the results showed no incidence of increased micronucleated polychromatic erythrocytes (MNPCE). These results indicate that PAT might not have mutagenic potential in vitro and vivo systems.


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