Effect of agrochemicals on mycelial growth and spore germination of a hyperparasite, Ampelomyces quisqualis 94013 for controlling cucumber powdery mildew

농약이 오이흰가루병 방제용 중복기생균 Ampelomyces quisqualis 94013의 균사생장 및 포자발아에 미치는 영향

  • Lee, Sang-Yeob (Division of Plant Pathology, National Institute of Agricultural Science and Technology, Rural Development Administration) ;
  • Lee, Sang-Bum (Research Policy Planning Division, Research Management Bureau, Rural Development Administration) ;
  • Kim, Yong-Ki (Division of Plant Pathology, National Institute of Agricultural Science and Technology, Rural Development Administration) ;
  • Kim, Hong-Gi (Department of Agricultural Biology, Chungnam National University)
  • 이상엽 (농촌진흥청 농업과학기술원 식물병리과) ;
  • 이상범 (농촌진흥청 연구개발국 연구정책과) ;
  • 김용기 (농촌진흥청 농업과학기술원 식물병리과) ;
  • 김홍기 (충남대학교 농과대학 농생물학과)
  • Published : 2004.03.30


A promising hyperparasite, Ampelomyces quisqualis 94013(AQ94013) was selected as a biological control agent to cucumber powdery mildew caused by Sphaerotheca fusca. Effect of agrochemicals on mycelium growth and spore germination of AQ94013 and effect of spread stickers on hyperparasitical activity of AQ94013 to powdery mildew pathogen were evaluated. Finally it was confirmed that mycelial growth and spore germination of AQ94013 on potato dextrose agar amended with two fungicides for controlling powdery mildew, triadimefon and pyrazophos; five fungicides for controlling downy mildew, dimethomorph, kasugamycin+copper oxychloride, dichlofluanid+copper oxychloride and tribasic copper sulfate; three fungicides for controlling gray mold, iprodione, vinclozolin and procymidone; and six insecticides immidacloprid, teflubenzuron, bifenthrin, ethofenprox, deltamethrin and phenthoate were slightly reduced. Addition of mineral oil in the spore suspension of AQ94013 enhanced 7.9% control value to cucumber powdery mildew.


  1. Epton, H. A. S. and Y. F. Hamed EI Nil (1993a) Improved hyperparasitism in Ampelomyces quisqualis, biocontrol agent of cucumber powdery mildew. Abstracts of the 6th Int. Congr. Plant Pathol., Montreal, p.64
  2. Feldman, K., M. Keren-zur, R. Hofstein and B. Fried1ender (1993) Ampelomyces quisqualis , an important component of an IPM program for the control of powdery mildew. Abstracts of the 6th Int. Congr. Plant Pathol. Montreal, p. 58
  3. Lee S. Y. and Kim H. G. (2001) Parasitic characteristics of Ampelomyces quisqualis 94013 to powdery mildew fungus of cucumber. The Korean J. of Mycology 29(2):116-12
  4. 中澤靖彦, 大緣範夫 (1994) ウリ類 うどんこ 病菌. 植物防疫 48(6):36-38
  5. Nelson, M. D. and W. D. Gubler (1995) Evaluation of biological and chemical agents for control of strawbe$\pi$y powdery mildew, Sphaerotheca macularis f.sp. fragariae. Phytopathology 85:1041(Abstr.)
  6. Shin, H. D. and H. Y. Kyeung (1994) Isolation of hyperparasitic fungi to powdery mildews and selection of superior isolate for biocontrol of cucumber powdery mildew. RDA Journal of Agricultural Science 36:141-151
  7. Sztejnberg, A. and S. Mazar (1985) Biocontrol of cucumber and carrot powdery mildew by Ampelomyces quisqualis. Phytopathology 75:1301-1302
  8. Epton, H. A. S. and Y. F. Hamed EI Nil (1993b) Improvement in the hyperparasitic activity of Ampelomyces quisquslis in the biocontrol of powdery mildew of cucumber. IOBC/WPRS Bulletin 16:86-89
  9. Philipp, W. D. and G. Cruger (1979) Parasitismus von Ampelomyces quisqualis auf Echten Mehltaupilzen an Gurten und anderen Gemusearten. Z. PfIanzenkrarnkh Pflanzensch. 86:129-142
  10. Philipp, W. D., U. Grauer and F. Grossmann (1984) Additional investigations on the biological and integrated control of cucumber powdery mildew under glass by Ampelomyces quisqualis. Z. PfIanzenkrarnkh. Pflanzensch. 91:438-443
  11. Shishkoff, N. and M. T. McGrath (1996) In vitro evaluation of AQ10 biofungicide with the fungicides myclobutanil and triadimefon or with AddQ spray adjvant for control. Phytopathology 86:53
  12. Erickson, E. O. and Wilcox, W. F. (1997) Distributions of sensitivities to three sterol demethylation inhibitor fungicides among populations of Uncinula necator sensitive and resistant to triadimefon. Phytopathology 87:784-791 https://doi.org/10.1094/PHYTO.1997.87.8.784
  13. Beloanger, R. R., A. J. Dik and J. M. Menzies (1998) Powdery mildews recent advances toward integrated control. In Plant-microbe Interactions and Biological Control.(Ed. Boland, G. J. and Kuykendall, D, L.). Marcel Dekker, NewYork
  14. Sztejnberg, A., S. alper, S. Mazar and N. Lisker (1989) Ampelomyces quisqualis for biological and integrated control of powdery mildews in Israel. J. Phytopath. 124:285-295
  15. Asari, S., H. Horie and Y. Nakazawa (1994) Current status in sensitivity of Sphaerotheca fuliginea to DMI in Kanto-Tooan District, Japan. Proc. Kanto-Tosan Plant Protec. Soc. 41:69-75
  16. Falk, S. P., D. M. Gadoury, Cortesi, P., R. C. Pearson and R. C. Seem (1995) Parasitism of Uncinula necator cleistothecia by the mycoparasite Ampelomyces quisqualis. Phytopathology 85:794-800 https://doi.org/10.1094/Phyto-85-794
  17. Pasini. C., D. F. Aquila, P. Curir and M. L. Gullino (1997) Effectiveness of antifungal compounds against rose powdery mildew(Sphaertheca pannosa var. rosae) in glasshouses. Crop-Pritection1 6:251-256
  18. Philipp, W. D. and A. Hellstern (1986) Biological control of powdery mildew by Ampelomyces quisqualis under reduced air humidity. Z. PfIanzenkrarnkh. Pflanzensch. 93:384-391
  19. Wright, D. P., D. Scholes, P. Horton, B. C. Baldwin and M. C. Shepphard (1990) In Current Reaserch in Photosynthesis, The relationship between the development of houstoria of Erysiphe graminis and the energy status of leaves. pp.223-226. vol. 4. M. Baltscheffsky, ed. Kluwer Academic Publishers, Dordrecht, Netherlands
  20. 中澤靖彦 (1995) キコウリ うどんこ 病菌の 藥濟 感受性. 今月の 農業(4): 114-118
  21. Hajlaoul, M. R. and R. R. Belanger (1991) Comparative effects of temperature and humidity on the activity of three potential antagonists of rose powdery mildew. Neth. J. Pl. Path. 97:203-209
  22. Philipp, W. D. (1978) Ampelomyces quisqualis mycoparasitism on powdery mildew of cucumber. Abstracts of the 3rd Int. congr. Plant Pathol. Munchen, p.201
  23. Sundheim, L. nd T. Amunden (1982) Fungicide tolerance in the hyperparasite Ampelomyces quisqualis and .integrated control of cucumber powdery mildew. Acta Agri. Scand. 32:349-355