Isolation and Identification of Antimicrobial Active Substances from Rhodiola sachlinensis

홍경천(Rhodiola sachlinensis)에서 항균성 물질의 분리 및 동정

  • 심창주 (충남대학교 식품공학과) ;
  • 이규희 (충남대학교 식품공학과) ;
  • 정재홍 (충남대학교 식품공학과) ;
  • 이상덕 (충남대학교 식품공학과) ;
  • 김영호 (충남대학교 약학대학 약학부) ;
  • 오만진 (충남대학교 식품공학과)
  • Published : 2004.03.01

Abstract

The antimicrobial substances from Rhodiola sachalinensis were extncted, isolated and identified. The highest level of antimicrobial activity and its yield were obtained in methanol extract. The minimum inhibition concentrations of the methanol extract were 500 $\mu\textrm{g}$mL on agar plate and 100 $\mu\textrm{g}$mL in broth media for four gram positive and four gram negative microbials. The methanol extract was fractionated by n-hexane, chloroform, ethyl ether, ethyl acetate, and butanol, orderly. The separate was developed on the TLC plate with different solvent system ratio of chloroform and methanol. Nine substances were isolated from chloroform and methanol mixture(9:1, v/v). Among them, three isolates showed antimicrobial activity. Three substances separated by HPLC were identified by GC/MS(EI) spectrum and $^1$H, /sup13/C-NMR spectrum. They were gallic acid, (-)-epicatechin and kaempferol. The antimicrobial activities of each substances were shown gallic acid, (-)-epicatechin, kaempferol orderly.

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