Studies on the Composition and Antioxidative Effect of Leaves form Korean Rosa davurica Pall.

한국산 생열귀나무(Rosa davurica Pall.) 잎의 성분 및 항산화 효과에 관한 연구

  • 김준범 (강원대학교 바이오산업공학부) ;
  • 최승필 (강원대학교 바이오산업공학부) ;
  • 이득식 (동해대학교 외식산업학과) ;
  • 함승시 (강원대학교 바이오산업공학부)
  • Published : 2004.03.01

Abstract

The objective of this study was carried out to investigate nutritional characteristics and biological activities effects of Korean leaf of Rosa davurica Pall. in vitro. They were extracted with methanol and then further fractionated to n-hexane, chloroform, ethyl-acetate, n-butanol and water from methanol extracts. Methods of the antigenotoxic used in this experiment were UVA/UVB absorption property and DPPH radical scavenge. The proximate compositions of leaves of Rosa dauvrica Pall were 67.5% of crude Moisture, 0.7% of crude fat, 6.8% of crude protein, 6.1% of crude ash, and 20.8% of crude fiber. The major minerals were K (1637.2 mg%), Ca (219.5 mg%), P (182.1 mg%), and Mg (135.1 mg%). Most of the fractions of methanol extract which leaves of Rosa dauvrica Pall. have strong absorbency at UVB region (308 nm) and UV A region (350nm). These fractions have a good absorbency property as synthetic filter and could be served as substitutes for synthetic UV sunscreen agents. All fractions (n-hexane, ethyl-acetate, n-butanol and water) from methanol extracts except chloroform fraction exhibited DPPH radical scavenging activity with IC$\_$50/ of 35.3, 6.0, 14.0, and 18.0 $\mu\textrm{g}$/mL.

References

  1. Chshman, D.W. and Ondetti, M.A. (1999) Design of angiotensin converting enzyme inhibitors. Nature Med. 5, 1110-1112 https://doi.org/10.1038/13423
  2. 서영준 (1997) 식품을 이용한 화학적 암 예방. 식품과학과 산업, 30, 59-63
  3. Ohara, T., Yoneyama, T., Ohike, T., Miyazaki, T. and Negishi, M. (1980) Quantitative detennination of rice or buckwheat flour contained in wheat flour by urea polyacrylamide gel electrophoresis. Nippon Shokuhin Kogyo Gakkaishi, 27, 426-432 https://doi.org/10.3136/nskkk1962.27.9_426
  4. 최용화, 김명조, 이행순, 곽상수 (1997) 해당화(Rosa rugosa) 잎의 항산화 물질. 생약학회지, 28, 179-184
  5. 食品醫藥品安全廳. (2000) 食品公典(別冊). 문영사, 서울, p.69-72, p273-277
  6. Buring, J.E. and Hennekens, C.H. (1997) Antioxidant vitamins and cardiovascular disease. Nutr. Rev., 55(II), S53-S60
  7. 육창수 (1989) 원색한국약용식물도감, p.272
  8. Varian Manual (1979) Analytical Methods for Flame Spectro- scopy. Varian Techtron PTY, LTD, Springvale, p.9
  9. Choi, J.S., Park, J.H., Kim, H.G., Young, H.S. and Mun, S.I. (1993) Screening for antioxidant activity of plants and marine algae and its active principles from Prunus aviana,. Kor. J. Pharmacology, 24, 299-303
  10. Bobin, M.F., Raymond, M. and Martini, M.C. (1994) UVA and UVB absorption properties of natural products, Cosmetics & Toiletries, 109, 63-70
  11. Chen, C.K. and Pace-Asciak, C.R. (1996) Vasorelaxing activity of resveratrol and quercetin in isolated rat aorta. Gen. Pharmacol., 27, 363-366 https://doi.org/10.1016/0306-3623(95)02001-2