Studies on the Optimal Culture Condition for Production of Red Pigments by Monascus rubber on Liquid Culture

액체배양에 의한 Monascus rubber의 적색 색소 생산을 위한 최적 배양조건에 관한 연구

  • 서승교 (대구산업정보대학 식품영양과) ;
  • 이창호 (경북대학교 식품공학과) ;
  • 우철주 (경북대학교 식품공학과)
  • Published : 2004.03.01


The optimum cultural conditions for production of red pigment from Monascus rubber KCTC 6122 is liquid culture were studied. Monascus robber KCTC 6122 was shown to give the maximum production of red pigment in the medium containing 4% rice powder, 0.2% NaNO$_3$, 0.3% Na$_2$HP0$_4$ and 0.15% MgSO$_4$. The optimum culture conditions, temperature, initial pH and shaking speed were 30$^{\circ}C$, 6.5 and 150 rpm, respectively. The red pigment production reached a maximum level at 8days of cultivation.


  1. Kim, C.S., Rhee, S.H. and Kim, I. (1977) Studies on production and characteristics of edible red color pigment produced by mold(Monascus sp.). Kor. J. Food Sci. Technol., 9, 277-283
  2. Manchand, P.S. and Whalley, W.B. (1973) Isolation and structure of ankaflavin a new pigment from Monascus anka. Phytochemistry. 12, 2531-2537
  3. Keisuke, T., Tomio, I., Nobukazu, T., Hiroshi, O., Shirou,A., Shouichi, T. and Yasue, N. (1992) Effect of mycelial weight on hypotensive activity of Beni-koji in spontaneously hypertensive rats. Nippon Shokuhin Kogyo Gakkashi., 39, 790-796
  4. Carels, M. and Shepherd, D. (1977) The effect of different nitrogen sources on pigment production and sporulation of Monascus sp. it submerged shaken culture. Can. J. Microbial., 23, 1360-1365
  5. Lin, C.F. (1973) Monascus sp. for pigment production. J. Ferment. Technol., 51, 407-412
  6. Evans, P.J. and Wong, H.Y. (1984) Pigment production from immobilized Monascus sp. utilizing polymeric resin adsorption. Appl. Environ. Microbial., 47, 1323-1326
  7. Juzlova, P., Martinkova, L. and Kren, V. (1996) Secondary metabolites of the fungus Monascus: a review. J. Ind. Microbial., 16, 163-170
  8. Su. Y.C. (1983) Fermentative production of anka-pigments(Monascus-pigments). 11, 325-337
  9. Yoshimura, M., Yamanaka, S., Mitsugi, K. and Hirose, Y. (1975) Production of Monascus-pigment in submerged culture. Agri. Biol. Chem., 39, 1789-1795
  10. Kim, H.S., Kwak, H.S., Yang, H.S., Pyun, Y.R. and Yu, J.H. (1979) Studies on the red pigment produced by Monascus sp. in submerged culture. Kor. J. Appl. Microbial. Bioeng., 7, 23-30
  11. Birch, A.J., Cassera, A., Fitton, D., Holker, J.S.E., Smith, H., Tompson, G.A. and Whalley, W.B. (1962) Studies in relation to biosynthesis. Rotiorin, monascin and rubropunctatin. J. Chem. Soc., 11, 3583-3587
  12. Lin, T.F. and Demain, A.L. (1991) Effect of nutrition of Monascus sp. on formation of red pigments. Appl. Microbial. Biotechnol., 36, 70-75
  13. Fielding, B.C., Haws, E.J., Holker, J.S.E., Powell, A.D.G., Robertson, A., Stanway, D.N. and Whalley, W.B. (1960) Monascorubin. Tetrahedron Lett., 5, 24-30
  14. Fielding, B.C., Holker, J.S.E., Jones, D.F., Powell, A.D.G., Richmond, I.W. and Whalley, W.B. (1961) The chemistry of fungi. The structure of monascin. J. Chem. Soc., 10, 4579-4582
  15. Johns, M.R. and Stuart, D.M. (1991) Production of pigments by Monascus purpureus in solid culture. J. Indust. Microbial., 8, 23-29
  16. Kim, H.S., Kwak, H.S., Yang, H.S., Pyun, Y.R. and Yu, J.H. (1979) Studies on the red pigment produced by Monascus sp. in submerged culture. Production of crude pigment, physical and physiological characteristics. Kor. J. Appl. Microbial. Bioeng., 7, 31-32
  17. Broder, C.U. and Koehler, P.E. (1980) Pigments produced by Monascus purpures with regard to quality and quantity. J. Food Sci., 45, 567-569
  18. Nakazawa, R. and Sato, K. (1930) On the Monascus of Taiwan the red seed of rice. J. Agric. Chem. Soc. Jpn., 6. 353-355
  19. Hirai, T., Shima, T., Suzuki, T., Tsukioka, M. and Ogasawara, N. (1979) Hyperpigment-productive mutant of M. anka for solid culture. Agric. biol. Chem., 43, 1975-1979
  20. Haws, E.J., Holker, J.S.E., Powell, A.D.G. and Robertson, A. (1959) The chemistry of fungi. The structure of rubropunctatin. J. Chem. Soc., 8, 3598-3603
  21. Lin, C.F. and Iizuka, H. (1982) Production of extracellular pigment by mutant of Monascus Kaoliang sp. nov. Appl. Environ. Microbial., 43, 671-676
  22. Hadfield, J.R., Holker, J.S.E. and Stanway, D.N. (1967) The biosynthesis of fungal metabolites. The $\beta$-oxo-lactone equivalents in rubropunctatin and monascorubrin. J. Chem. Soc., 16, 751-754
  23. Nishikawa, E. (1932) Studies on the biochemistry of mold. The pigments of Monascus purpureus Went. J. Agric. Chem. Soc. Jpn., 8. 1007-1011
  24. Park, Y.H. (1997) Production of yellow and red pigments by Monascus purpureus in culture conditions. Soonchunhyang J. Nat. Sci., 3, 703-707
  25. Lin, C.F. (1973) Isolation and cultural conditions of Monascus sp. for the production of pigment in a submerged culture. J. Ferment. Technol., 51, 107-114
  26. Wong, H.C. and Bau, T.S. (1977) Pigmentation and antibacterial activity of fast neutron- and X-ray-induced strains of Monascus purpures Went. Plant. Physiol., 60, 578-581