Analysis of Genetic Diversity of Bifidobacterium spp. Isolated from Korean Adults Fecal Samples

한국 성인 분변 시료에서 분리한 비피더스균의 유전자 다양성 분석

  • Published : 2004.02.01


Twelve strains of Bifidobacterium spp. were isolated from the feces of healthy Korean 20∼30 years. The identification of genera from isolates were performed by the microscopic observation and fructose-6-phosphate phosphoketolase (F6PPK) activity which is the key enzyme to distinguish the Bifidobacterium spp. from other anaerobic bacteria. To determine the antibacterial resistance patterns, minimum inhibitory concentration (MIC) of several antibiotics (including anti-tuberculosis agents) was determined. Five of the isolate!, showed the high degree of resistance to vancomycin. To investigate the genetic diversity between isolates and type strain of Bifidobacterium spp. from KCTC, we peformed the RAPD-fingerprinting. Using a total set of four primers, it is possible to distinguish the isolates and Bifidobacterium spp. from KCTC. Thus, Bifidobacterium strains isolated from our samples may be a new species or strains of Bifidobacteriurn genera, and have the potential as a probiotics.


  1. J. Appl. Microbiol. Biotechnol. v.22 Composition and distribytion of intestinal microbial flora in Korean Ji,G.E.
  2. J. Food Sci. v.28 In vitro digestibility of protein in yoghurt at various stages of processing Breslaw,E.S.;Kleyn,D.H.
  3. Arch. Pharm. Res. v.25 Hypocholesterolemic activity of Bifidobacteria isolated from a healthy Korean Rhee,Y.K.;Han,M.J.;Choi,E.C.;Kim,D.H.
  4. Ann. Med. v.22 Prevention of travelers diarrhoea by Lactobacillus GG Oksanan,P.J.;Salminen,S.;Saxelin,M.;Hamalainen,P.
  5. Am. J. Gastroenterol. v.Suppl 40 Lactic acid-mediated suppression of Helicobacter pylori by the oral administration of Lactobacillus salivarius as a probiotic in a gnotobiotic murine model Aiba,Y.;Suzuki,N.;Kabir,A.M.;Takagi,A.;Koga,Y.
  6. Gut v.41 Prevention of Helicobacter pylori infection by lactobacilli in a gnotobiotic murine model Kabir,A.M.;Aiba,Y.;Takagi,A.;Kamiya,S.;Miwa,T.;Koga,Y.
  7. J. Appl. Bacteriol. v.79 In vitro inhibition of Helicobacter pylori NCTC 11637 by organic acids and lactic acid bacteria Midolo,P.D.;Lambert,J.R.;Hull,R.;Luo,F.;Grayson,M.L.
  8. Cancer Res. v.45 A new morphologically characterized cell wall preparation (whole peptidoglycan) from Bifidobacterium infantis with a higher efficacy on the regression of an established tumor in mice Sekine,K.;Toida,T.;Saito,M.;Kuboyama,M.;Kawashima,T.;Hashimoto,Y.
  9. Bull. Int. Union Tuberc. Lung Dis. v.63 Antituberculosis regimens of chemotherapy Committee on Treatment of International Union against Tuberculosis and Lung Disease
  10. Bifidpbacteria Microflora v.4 Effects of Bifermin-R admistered in combination with antibiotics on the fecal flora Yamashita,M.;Hatano,M.;Matsui,T.;Kumon,Y.
  11. Bifidobacterium. Bergeys Manual of Systematic Bacteriology(9th ed.) v.2 Scardovi,V.;Sneath,P.H.(ed.);Mair,N.S.(ed.);Sharpe,M.E.(ed.);Holt,J.G.(ed.)
  12. Approved standard M7-A2(2nd ed.) Methods for dilution antimicrobial susceptibility tests for bacteia that grow anerobically National Committee for Clinical Laboratory Standards
  13. Current protocols in Molecular Biology v.1 Ausble,F.M.;Brent,R.;Kingston,R.E.;Moore,D.D.;Seidman,J.G.;Smith,J.A.;Struhl,K.
  14. Yakhak Hoeji v.42 Antibiotic susceptibility of Bifidobacterium spp. strains isolated from healthy Korean Chang,H.A.;Choi,K.H.;Oh,T.K.;Kwon,A.R.;Kim,D.H.;Choi,E.C.
  15. J. Appl. Bacteriol. v.60 DNA:DNA homology, physiological characteristics and distribution of lactic acid bacteria isolated from maize silage Dellaglio,F.;Torriani,S.
  16. Int. J. Sys. Bacteriol. v.40 Restriction endonuclease patterns and multivariate analysis as a classification tool for Lactobacillus spp Stahl,M.;Molin,G.;Persson,A.;Ahrne,S.;Stahl,S.
  17. Nucleic Acids Res. v.18 DNA polymorphisms amplified by arbitrary primers are useful as genetic markers Williams,J.G.K.;Kubelik,A.R.;Livak,K.J.;Ragalski,J.A.;Tingey,S.V.
  18. Plant Cell Report v.15 Genetic diversity of potato determined by random amplified polymorphic DNA analysis Demeke,T.;Lynch,D.R.;Kawchuck,L.K.;Kozub.G.C.;Amstrong,J.D.
  19. Can. J. Microbiol. v.44 Random amplified polymorphic DNA analysis and demonstration of genetic variability among bifidobacteria isolated from rats fed with raw kidney beans Fanedl,L.;Nekrep,F.V.;Avgustin,G.
  20. J. Microbiol. v.38 Characterization of isolated Lactobacillus spp. and classification by RAPD-PCR analysis Kwon,O.S.
  21. Arch. Pharm. Res. v.26 Molecular differentiation of Panax species by RAPD analysis Shim,Y.H.;Choi,J.H.;Park,C.D.;Lim,C.J.;Cho,J.H.;Kim,H.J.
  22. Int. J. Food. Microbiol v.43 Characterization of Bifidobacteria by random DNA amplification Vincent,D.;Roy,D.;Mondou,F.;Dery,C.
  23. Mol. Cells v.13 Fingerprinting of diverse genomes using PCR with universal rice primers generated from repetitive sequence of Korean weedy rice Kang,H.W.;Park,D.S.;Go,S.J.;Eun,M.Y.
  24. Arch. Pharm. Res. v.26 Antimicrobial resistance patterns of vancomycin-resistant Strptococcus equinus isolated from animal foods and epidemiological typing of resistant S. ewuinus by Microbial Uniprimer kit. Choi,S.S.;Lee,J.W.;Kang,B.Y.;Ha,N.J.
  25. Mycobiology v.29 Genomic differentiation among oyster mushroom cultivars released in Korean by URP-PCR fingerprinting Kang,H.W.;Park,D.S.;Park,Y.J.;You,C.H.;Lee,B.M.;Eun,M.Y.;Go.S.J.
  26. Korean J. Crop Sci. v.46 Phylogenic relationship of Allium species in subgenus Rhizirideum by PCR DNA fingerprint Kim,H.H.;Kang,H.W.;Park,Y.J.;Baek,H.J.;Gwag,J.K.
  27. Korean J. Horti. Sci. Technol. v.19 Discrimination of potato varieties by random amplified polymorphic DNA analysis Seo,H.W.;Yi,J.Y.;Cho,H.M.;Park,Y.E.;Oh,S.E.
  28. Int. J. Food Microbiol. v.36 Lactic acid bacteria of foods and their current taxonomy Stiles,M.E.;Holzapfel,W.H.
  29. Yakhak Hoeji v.37 Development of Bifidobacterium bifidum strains resistant to rifampicin Choi,E.C.;Ko,S.Y.;Kim,H.S.;Choi,S.S.;Kim,S.K.;Kim,B.K.
  30. Yakhak Hoeji v.38 Development of Bifidobacterium bifidum strains resistant to rifampicin and ofloxacin Chung,Y.J.;Jeon,M.I.;Kang,C.Y.;Kim,B.K.;Choi,E.C.