Antitumor and Antimutagenic Effect of the Proteinpolysaccharides from Polyporus umbellatus

저령다당체의 항종양 및 항돌연변이 효능

  • 이정화 (부산대학교 분자생물학과) ;
  • 신유진 (동서대학교 식품생명공학) ;
  • 조덕제 (동서대학교 식품생명공학) ;
  • 임희진 (동서대학교 식품생명공학) ;
  • 최원일 (대우약품 (주) ;
  • 이용규 (동서대학교 식품생명공학과)
  • Published : 2004.03.01


The proteinpolysaccharides (PPS) from Polyporus umbellatus (P. umbellatus) screlotium is composed by 78.2% of saccharide, 16.8% of protein, and 4.0% of ash. PPS from P. umbellatus showed antitumor activities against 180 solid tumor in ICR mice at the concentration of 20-160 mg/kg/day. PPS from P. umbellatus inhibited cell viability to 47.4% and 45.0% in leukemia cell lines, L-1210 and K-562 cells at 50-400 $\mu\textrm{g}$/mL concentration, respectively. But the hall mark of cell apoptosis, DNA fragmentation was not observed at those concentration. 2.5-10.0% of PPS from P. umbellatus inhibited mutagenecity evoked by 2-nitrofluorene and sodium azide in Salmonella typhimurium TA 98 and TA 100. From these results, it is suggested that the PPS of P. umbellatus has antitumor and antimutagenic effect, and its cytotoxic effect may not be ascribed to the apoptosis.


  1. Lu W, Adachi I, Kano K, Yasuta A, Torlizuka K, Ueno M. 1985. Pletelet aggregation potentiators from cho-rei. Chem Pharm Bull 33: 5083-5087.
  2. Sato K, Osawa M, Suzuki Y, Oikawa S. 1984. Difference in fruiting capability of stocks in Grifola Frondosa and its allied species. Trans Mycol Soc Japan 25: 205-209.
  3. Miyazaki T, Oikawa N, Yadomae T, Yamada H, Yamada Y. 1979. Relationship between the chemical structure and antitumor activity of glucans prepared from Grifolla umbellata. Carbohydrate Research 69: 165-170.
  4. Ueno Y, Okamoto Y, Yamauchi R, Kato K. 1982. An antitumor activity of the alkali-soluble polysaccharide (and its derivatives) obtained from the sclerotia of Grifora umbellata. Carbohydrate Research 101: 160-167.
  5. Zhang Y, Liu Y, Yan SC. 1991. Effect of Polyporus umbellatus polysaccahride on function of macrophages in the peritoneal cavities of mice with lesions. Cheih Ho Tsa Chih 11: 211-215.
  6. May L, Yun SC. 1990. Therapeutic investigation of Polyporus umbellatus polysaccahride on the children with pure immunity. J Traditional Chinese Medicine (China) 3: 168-169.
  7. Chang YF, Liu YY, Yun SC. 1991. Effect of Polyporus umbellatus polysaccahrides on macrophages of liver damaged mice. Intergrated J Chinese and Western Medicine (China) 11: 225-226.
  8. Xiong LL. 1993. Therapeutic effect of combined therapy of Salvia miltiorrhizae and Polyporus umbellatus polysaccharide in the treatment of chronic hepatitis B. Cheih Ho Tsa Chih 13: 516-517.
  9. You J, Hau D, Chen K, Huang H. 1994. Combined effects fo chulling (Polyporus umbellatus) extract and mitomycin C on experimental liver cancer. Am J Chin Med 22: 19-28.
  10. Ishida H, Inaoka Y, Shibatani J, Fukushima M, Tsuji K. 1999. Studies of the active substances in herbs used for hair treatment II Isolation of hair regrowth substances acerosyringone and polyporusterone A and B from Polyporus umbellatus fries. Biol Pharm Bull 22: 1189-1192.
  11. Ishida H. 1999. Studies of active substances in herbs used for hair treatment IV. The structure of the hair growth substance, polypurusterone A from Polyporus umbellatus fries. Chemical & Pharmaceutical Bulletin 47: 2059-2062.
  12. Wang DC, Lee PY, Wan YC. 1983. Some pharmacological actions of Polyporus umbellatus. Chinese Herb Medicine (China) 14: 267-268.
  13. Natural Products Research Institute. 1996. Tradimed. Seoul National University.
  14. Moon CK, Park KS, Lee SH, Yoon YP. 1985. Antitumor activities of several phytopolysaccharides. Arch Pharm Res 8: 42-46.
  15. Lee JH, Cho SM, Ko KS, Yoo IK. 1995. Effect of cultural conditions on polysaccharides production and its monosaccharide composition in Phellinus linteus L13202. Korean J Mycology 23: 325-329.
  16. Herbert D, Phipps PJ, Strange RE. 1971. Chemical analysis of microbial cell. In Methods in microbiolodsgy. Norris JR, Ribbons DW, eds. Academic Press Inc, New York. Vol 5b, p 265.
  17. Choi JH, Jang JC, Park KD, Park MH, Oh SK. 1981. High performance liquid chromatogtapic determination of sugars in ginseng and it's products. Korean J Food Sci Techool 13: 107-111.
  18. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. 1951. Protein measurement with the folin phenol reagent. J Biol Chem 193: 265-269.
  19. Mossman T. 1983. Rapid colorimetric assay for cellular growth and survival. Application to proliferation and cytotoxicity assays. J Immuno Methods 65: 55-63.
  20. Maron DM, Ames BN. 1983. Revised methods for the Salmonella mutagenecity test. Mutat Res 113: 173-178.
  21. Matsushima T, Sugimura T, Nagao M, Yahagi T, Shirai A, Sawamura M. 1980. Factors modulating mutagenecity in microbial test. In Short-term test systems for detecting carcinogens. Norpoth KH, Garner RC, eds. Berling, Sandiego. p 273.
  22. Hay RJ, Park JG, Gazdar A. 1994. Evaluation of physiological material. Academic Press, San Diego. p 34-36.
  23. Takatomi O, Masae Y, Chusei T, Mitsuo M, Hideo B. 1992. Studies on constituents of fruit body of Pollyporus umbellatus and their cytotoxic activity. Chem Pharm Bull 40: 143-147.
  24. Steller H. 1995. Mechanism and genes of cellular sucide. Science 267: 1445-1448.
  25. Im EO, Lee S, Suh H, Kim KW, Bae YT, Kim ND. 1999. A novel ursodeoxycholic acid derivative induced apoptosis in MCF-7 human breast cancer cells. Pharm Pharmacol Commun 5: 1-7.
  26. Ewa S, Anna BZ, Katarzyna P, Janusz S, Ewa R. 1997. Inhibition of proliferation and apoptosis of human and rat T lymphocytes by curcumin, a curry pigment. Biochem Pharmacol 54: 899-907.

Cited by

  1. Antioxidant and antiproliferating effects of Setaria italica, Panicum miliaceum and Sorghum bicolor extracts on prostate cancer cell lines vol.23, pp.7, 2016,
  2. Enhancement of antimicrobial and antimutagenic activities of Korean barberry (Berberis koreana Palib.) by the combined process of high-pressure extraction with probiotic fermentation vol.90, pp.14, 2010,