DOI QR코드

DOI QR Code

Cytotoxicity and Chemosensitizing Effect of Camellia(Camellia japonica) Tea Extracts

동백엽차와 화차의 세포독성 및 다제내성 극복효과

  • 황은주 (동신대학교 생물자원산업화지원센터) ;
  • 차영주 (동신대학교 산업용가속기 이용 생물연구센) ;
  • 박민희 (동신대학교 산업용가속기 이용 생물연구센) ;
  • 이장원 (동신대학교 산업용가속기 이용 생물연구센) ;
  • 이숙영 (동신대학교 산업용가속기 이용 생물연구센터)
  • Published : 2004.03.01

Abstract

This study has been undertaken to increase availability of native camellia in Jeonnam as a medicinal resource and to isolate the effective components from them. Fresh leaf and flower of camellia, single camellia tea and camellia tea mixed with green tea, herbs were screened for cytotoxicity on MCF -7 (human breast adenocarcinoma pleual effusion), Calu-6 (human pulmonary carcinoma), SNU-601 (human gastric carcinoma) cells. Also their multidrug-resistance reversing activity were evaluated using drug sensitive AML-2/WT and multidrug-resistant AML-2/D100 cells. Among the camellia extracts, young leaf and camellia tea mixed with green tea had strong growth inhibitory effects in below 100 $\mu\textrm{g}$/mL against human cancer cells. In result, young leaf showed the strongest inhibitory effects on MCF -7 ($IC_{50}$/ = 100 $\mu\textrm{g}$/mL ↑), Calu-6 ($IC_{50}$/ = 79 $\mu\textrm{g}$/mL), and SNU -601 ($IC_{50}$/ = 39 $\mu\textrm{g}$/mL), and AML-2/WT ($IC_{50}$/ = 64 $\mu\textrm{g}$/mL). Chemosensitizing effect was the extracts of mature leaf ($IC_{50}$/ = 97 $\mu\textrm{g}$/mL, RF=3.0), roasted tea ($IC_{50}$/ = 76 $\mu\textrm{g}$/mL, RF = 2.6 ↑) and steam tea ($IC_{50}$/ = 70 $\mu\textrm{g}$/mL, RF=2.8 ↑) strongly potentiate vincristine cytotoxicity in AML-2/D100 cells. But their cytotoxicities to both sensitive AML-2/WT and resistant AML-2/D100 cells were in the same order of magnitude. This results indicate that crude extracts of camellia mature leaves would contain some principles which have chemosensitizing activity.

References

  1. Kim SE, Hwang BY, Kim YH, Kim YC, Lee KS, Lee JJ. 1997. Multidrug-resistance reversing activity of medicinal plants. Kor J Pharmacogn 28: 174-178.
  2. 문교부. 1974. 한국동식물도감. 삼화출판사, 서울. 제15권, p 665.
  3. 송규택, 정현배, 봉희성. 1984. 한국자원식물, 미도문화사. p 650.
  4. 송규택. 1978. 한국자원식물. 한국자원식물연구소. p 650.
  5. 이창복. 1982. 대한식물도감. 향문사, 서울. p 543.
  6. Itokawa H, Nakajima H, Ikuta A, Iitaka Y. 1981. Two teiterpenes from the flowers of Camellia japonica. Phytochemistry 20: 2539-2542. https://doi.org/10.1016/0031-9422(81)83089-0
  7. Bhakuni DS, Goel AK, Jain S, Mehrotra BN, Patnaik GK. Prakash V. 1988. Screening of Indian plants for biological activity (Part III). J Exp Biol 26: 883-886.
  8. Yoshikawa M, Harada E, Murakami T, Matsuda H, Yamahara J, Murakami N. 1994. Camelliasa ponins B1, B2, C1 and C2, new type inhibitors of ethanol absorption in rats from the seeds of Camellia japonica L. Chem Pharm Bull 42: 742-746. https://doi.org/10.1248/cpb.42.742
  9. Fujita Y, Fujita S, Yoshikawa H. 1973. Comparative biochemical and chemotaxonomical studies of the plants of Theaceae (I). Essential oils of Camellia sasanqua Thunb., C. japonica Linn., and Thea sinensis Linn. Osaka Kogyo Gijutsu Shidensho Kigo 25: 198-203.
  10. 최옥자, 김용두, 강성구. 1996. 동백종실 및 유박의 활용방안, 고유 농수산품목 세계화 대상품목의 연구조사, 동백나무편. 전라남도. p 331-425.
  11. Kim JH, Lee SY, Cho SI. 2003. Anti-proliferative effect of Camellia japonica leaves on human leukemia cell line. Kor J Herbology 18: 93-98. https://doi.org/10.1111/j.1748-5967.2003.tb00056.x
  12. Hwang EJ, Lee SY. 2003. Chemosensitizing activity against human leukemia cell of crude extracts of native camellia (Camellia japonica) in Jeonnam. 2003 Annual Meeting of the Korean Society of Applied Pharmacology. PB-11, p 85.
  13. Hwang EJ, Lee SY, Jeon WB, Pyo BS, Kim SM. 2003. Inhibitory effect of camellia (Camellia japonica) extract on growth of various cancer cells. 2003 Annual Meeting of The Korea Society of Medicinal Crop Science. Vol 11, Supplement No 2, p 220-221.
  14. Lee SY, Cha YJ, Lee JW, Hwang EJ, Kwon SJ, Cho SI. 2003. Physiological and pharmacological activities of nutraceutical tea by leaves and flowers of domestic camellia (Camellia japonica) The Plant Resources Society of Korea. The 10th International Symposium. No.16, Korean Supplement 2, p 48-49.
  15. Kim JH, Im WC, Park MH, Lee JH, Lee SY. 2003. Optimized conditions for making tea from camellia (Camellia japonica) leaf and flower and sensory evaluation. The Plant Resources Society of Korea. The 10th International Symposium. No. 16, Korean Supplement 2, p 34-35.
  16. Cha YJ, Lee JW, Park MH, Hwang EJ, Lee SY. 2003. Major composition of leaf tea and flower tea using native camellia (Camellia japonica) in Korea, The Plant Resources Society of Korea. The 10th International Symposium. No.16, Korean Supplement 2, p 33.
  17. Hwang EJ, Park MH, Pyo BS, Cha YJ, Lee SY. 2003. The cytotoxicity and chemosensitizing effects of native camellia (Camellia japonica) and nutraceutical camellia teas. 2003 Annual Meeting of The Plant Resources Society of Korea. No. 16, Korean Supplement 1, p 102.
  18. Cha YJ, Park MH, Cho SI, Kim JH, Lee SY. 2002. The development of nutraceutical tea by native camellia in Korea. Annual Meeting and Seminar of the Korean Camellia Research Society.
  19. Fischer SM, Leyton LJ, Lee ML, Locnislcar M, Belury MA, Maldve RE. 1992. Differential effects of dietary linoleic acid on mouse skin-tumor promotion and mammary carcinogenesis. Caner Res (Suppl) 52: 2049-2056.
  20. Kim HJ, Kim MK. 2003. Anticancer effect of persimmon leaf extracts on Korean gastric cancer cell. J Kor Soc F Nutr 36: 133-146.
  21. Ahmad N, Feyes DK, Nieminen AL, Agarwal R, Mukhtar H. 1997. Green tea constituent epigallocatechin-3-gallate and induction of apoptosis and cell cycle arrest in human carcinoma cells. J Natl Cancer Inst 89: 1881-1886. https://doi.org/10.1093/jnci/89.24.1881
  22. Tanaka H, Hirose M, Kawabe M, Sano M, Takesada Y, Hagisara A, Shirai T. 1997. Post-initiation inhibitory effects of green tea catechins on 7, 10-dimethylbenz[a]anthraceneinduced mammary gland carcinogenesis in female Sprague-Dawley rats. Cancer Lett 116: 47-52. https://doi.org/10.1016/S0304-3835(97)04749-6
  23. Chang IL, Chi HJ. 1982. Toxicological evaluation of medicinal plants used for herbal drugs (III). Kor J Pharmacog 13: 55-61.
  24. Kim JR, Kim SY, Kim JH. 1995. Reversal effect of antihistamines on multidrug resistance in adriamycin- and vincristine-resistant L1210 variants. J Kor Cancer Assoc 27: 130-137.

Cited by

  1. Effects of Extract from Fermented Flower-buds of Panax ginseng C.A. Meyer on Mouse Cytokine IL-6, TNF-α Production vol.27, pp.1, 2014, https://doi.org/10.9799/ksfan.2014.27.1.043
  2. Antioxidative and Antimutagenic Effects and Hyperplasia Inhibitory Activity of Cancer Cells from Luffa cylindrica Extracts vol.25, pp.4, 2012, https://doi.org/10.9799/ksfan.2012.25.4.888
  3. Radiation Synthesis and Anti-inflammatory Evaluation of Polysaccharide Hydrogels from Ulmus Davidiana Var. Japonica vol.38, pp.1, 2014, https://doi.org/10.7317/pk.2014.38.1.69
  4. Antioxidant and antiproliferating effects of Setaria italica, Panicum miliaceum and Sorghum bicolor extracts on prostate cancer cell lines vol.23, pp.7, 2016, https://doi.org/10.11002/kjfp.2016.23.7.1033
  5. Effect of Corn Extracts on Mouse IL-2 Cytokine Production by Peritoneal Macrophage and the Ratio of IFN-γ, IL-10 Cytokine vol.25, pp.2, 2012, https://doi.org/10.9799/ksfan.2012.25.2.362
  6. Identification of the biologically active constituents of Camellia japonica leaf and anti-hyperuricemic effect in vitro and in vivo vol.39, pp.6, 2017, https://doi.org/10.3892/ijmm.2017.2973
  7. Effects of Plant Water Extract Codonopsis Lanceolatae on Mouse Immune Cell Activation Ex Vivo vol.42, pp.3, 2009, https://doi.org/10.4163/kjn.2009.42.3.207