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Chemical Interaction of L-Ascorbic Acid with Ferritin Molecule

가시부영역의 흡수 스펙트럼 변화에 의한 L-Ascorbic Acid와 Ferritin과의 상호작용

  • 김미옥 (대구보건대학 보건다이어트과) ;
  • 나재철 (대구보건대학 보건다이어트) ;
  • 장상문 (대구보건대학 호텔조리음료계열)
  • Published : 2004.03.01

Abstract

This study consisted of designing a sensitive assay to measure the rate of L-ascorbic acid (AsA)-prompted release of ferritin iron, the use of ferrozine as a chelating agent to trap releases Fe(II). The initial rate of iron release was measured in the appearance of Fe(ferrozine)$_3$$^{2+}$ at 562 nm. The release of iron from ferritin by AsA was dependent on time and AsA conditions under aerobic and anaerobic conditions. Effect of oxygen on the release of iron from ferritin was also confirmed. It was suggested that the release of iron from ferritin was participate not only AsA but also $O_2$$^{[-10]}$ . In this study, it was found that iron can be released from ferritin and chelate as Fe(ferrozine)$_3$$^{2+}$ and the release was more than 50% in the presence of AsA without $O_2$$^{[-10]}$ . Based on the findings, the following can be assumed (1) AsA is diffused into ferritin (2) ferric ion is reduced to ferrous ion (3) is diffused from ferritin.tin.

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