Effect of pH on Continuous Hydrogen Fermentation

연속반응실험에서 수소생성에 대한 pH 영향

  • Lee, Young-Joon (Water Quality Research Department, National Institute of Environmental Research)
  • Published : 2004.06.01

Abstract

The influences of pH on hydrogen production were also investigated over the pH range from 4.1 to 8.0 at HRT 10 hours. The hydrogen content for the produced gas was changed from 41 to 71% with corresponding pHs throughout this experiment. The produced hydrogen/carbon dioxide ratio was not vary significantly up to 6.0, then steepenly increased with increases in the pH. The maximal hydrogen yield was found to be 3.16 $\ell$/g sucrose at pH 5.0. Acetate production yield increased with increased pH, but butyrate production yield decreased with increased pH. Biomass yield increased with increased pH.

References

  1. Zajic, J. E., Kosaric, N. and Brosseau, J. D.: Microbial production of hydrogen. Adv. Biochem. Eng., 7, 57-109, 1978
  2. Rajeshwar, K., Ibanez, J. G. and Swain, G. M. : Electrochemistry and the environment. J. Appl. Electrochem., 24, 1077-1091, 1994
  3. Kondratieva, E. N. and Gogotov, I.: Production of molecular hydrogen in microorganisms. Adv. Biochem. Eng., 28, 139-191, 1983
  4. Ueno, Y., Otsuka, S. and Morimoto, M.: Hydrogen production from industrialwastewater by anaerobic microflora in chemostat culture. J. Ferment. Technol, 82(2), 194-197, 1996
  5. Bowles, L. K. and Ellefson, W.: Effects of butanol on Clostridium acetobutyticum. Appl. Environ. Microbiol., 50, 1165-1170, 1985
  6. Dabrock, B., Bahl, H. and Gottschalk, G.: Parameters affecting solvent production by Clostridium pasteurianum. Appl. Environ. MicrobioI., 58(4), 1233-1239, 1992
  7. APHA: Standard methods for the examination of water and wastewater, 19th ed. Amencan Public Health Association, Washington, DC, USA, 2-57, 1995
  8. Dubios, M., Gilles, K. L., Hamilton, J. K., Rebers, P. A. and Smith, F.: Colorimetric method for determination of sugars and related substances. Anal. Chem., 28, 350-356, 1956 https://doi.org/10.1021/ac60111a017
  9. Crabbendam, P. M., Neijssel, O. M. and Tempest, D.W.: Metabolic and energetic aspects of the growth of Clostridium butyricum on glucose in chemostat culture. Arch. Microbiol., 142, 375-382, 1985 https://doi.org/10.1007/BF00491907
  10. Zoetemeyer, R. J., van den Heuvel, J. C. and Cohen, A.: pH influence on acidogenic dissimilation of glucose in an anaerobic digestor. Wat. Res., 16, 303-311, 1982 https://doi.org/10.1016/0043-1354(82)90190-7
  11. Kissalita, W. S., Pinder, K. L. and Lo, K. V.:Acidogenic fermentation of lactose, Biotechnol. Bioeng., 30, 88-95, 1987 https://doi.org/10.1002/bit.260300113
  12. Doelle, H. W.: Bacterial metabolism, (2nd ed). Academic, New York, 1979
  13. Atkinson, A. and Mavituna, F.: Biochemical engineering and biotechnology handbook, Nature, New York, 1983
  14. Bryant, M. P. : Microbial methane production-theoretical aspects. J. Anim. Sci., 48, 193-201, 1979 https://doi.org/10.2527/jas1979.481193x