Increased Stability of Bacillus polyfermenticus SCD in Low pH, High Temperature and High Glucose Concentration via Three Layer Coating

Bacillus polyfermenticus SCD의 Three Layer Coating에 의한 pH, 열, 높은 glucose 농도에 대한 안정성효과

  • 이진옥 (신라대학교 공과대학 생명공학과) ;
  • 전경동 ((주)바이넥스 중앙연구) ;
  • 강재선 ((주)바이넥스 중앙연구) ;
  • 이재화 (신라대학교 공과대학 생명공학과)
  • Published : 2004.06.01


Bacillus polyfermenticus SCD derived from Bacillus sp., which is commonly called as Bisroot$^{ⓡ}$. The goal of this study, is to Increase stability of Bacillus polyfermenticus SCD in low pH, high temperature and high glucose concentration via three layer coating. The viability of coated Bacillus polyfermenticus SCD increased to 30%, 20%, 14% in the condition of pH 2 4 6 than that of uncoated Bacillus polyfermenticus SCD at 37$^{\circ}C$ for 4 h. Final viability of the coated Bacillus polyfermenticus SCD in 80$^{\circ}C$ increased to 40% than that of uncoated Bacillus polyfermenticus SCD. In high glucose concentration coated Bacillus polyfermenticus SCD is more stable than uncoated about 50%. In conclusion, the three layer coated Bacillus polyfermenticus SCD is very stable for low pH, high temperature and high glucose concentration.


  1. Am. Soc. Microbiol. Molecular Genetics of Bacteria Snyder;W. Champness
  2. Kor. J. Microbiol.Biotechnol. v.30 Characterization of Bacillus polyfermenticus SCD as a Probiotic Jun, K. D.;H. J. Kim;K. H. Lee;H. D. Paik;J. S. Kang
  3. Indian Dairyman v.41 Therapeutic properties of acidophilus milk Khedkar, C. D.;J. M. Mantri;S. A. Kulkarnl
  4. J. Microbiol. Biotechnol. v.11 Improvement of the stability of Lactobacillus casei YIT 9018 by microencapsulation using alginate and chitosan Koo, S. M.;Y. H. Cho;C. S. Huh;Y. J. Baek;J. Y. Park
  5. A review. Can. J. Anim. Sci. v.72 Enzyme applications for monogastric feeds Campbell, G. L.;M. R. Bedford
  6. J. Appl. Bacteriol. v.66 Probiotics in man and animals Fuller, R.
  7. M. S. Thesis, Kyungnam University Microbiological Identification, Antimicrobial Activity and Enhanced Production of Bisroot Strain Jun, K. D.
  8. Kor. Patent Publ. No. 91004344 Lactobacillus sp. KCTC 8458 BP and it`s application Ko, Y. H.;T. K. Oh;Y. H. Park;Y. S. Kim;D. Y. Yoo;K. H. Cho
  9. Biotechnol. Lett. v.10 Effect of culture conditions on growth and sporulation of Bacillus thuringiensis subsp. israelensis and development of media for production of the protin crystal endotoxin Pcarson, D.;O. P Ward
  10. J. Microbiol. Biotechnol. v.10 Identification and partial characterization of cerein BS229, a bacteriocin produced by Bacillus cereus BS229 Paik, H. D.;N. K. Lee;K. H. Lee;Y. L. Hwang; J. C. Pan
  11. Kor. J. Microbiol. v.27 characteristics of Lactobacillus reuteri BSA-131 isolated from swine intestine Chang, Y. H.;J. K. Kim;J. H. Yoon;W. Y. Park
  12. Del. Med. J. v.42 The significance of the intestinal microflora Finegold, S. M.
  13. Korean. J. Food Sci. Technol. v.34 Characterization of Bacillus polyfermenticus SCD for oral bacteriotherapy of gastrointestinal disorders Paik, H. D.;M.Y. Jung;H. Y. Jung;W. S. Kim;K. T. Kim