$^2D$ NMR Probe Development for Investigation of Biosupramolecular Systems

  • Kim, Andre (Department of Chemistry, Pusan National University) ;
  • Kang, Shin-Won (Department of Chemistry, Pusan National University) ;
  • Park, Jang-Su (Department of Chemistry, Pusan National University)
  • Published : 2004.06.01


Biosupramolecular systems such as biological membranes usually fluid under physiological conditions$^1$. Therefore, solid-state NMR has been used to investigate biosupramolecular systems. But solid-state NMR spectra contain a large number of overlapping resonances and are rather difficult to analyze. These problem has to be overcome by selective isotope labeling. We constructed a deuterium NMR probe for AM400 NMR spectrometer, which is mainly used for liquid samples. To overcome the fluidity problem, a saddle type coil was designed. The efficiency was systematically investigated for two kinds of coil geometry, solenoid and saddle types. Our results suggest that solenoids are superior to saddle type coils in the sensitivity. However, the letter fits better to fluid samples such as biosupramolecular systems.