Determination of alkylphenol ethoxylate in water by high performance liquid chromatography/electrospray ionization/mass spectrometry

HPLC/ESI/MS를 이용한 물 중의 알킬페놀에톡실레이트 분석

  • Lee, Jeongae (Bioanalysis & Biotransformation Research Center, Korea Institute Science & Technology) ;
  • Park, Song-Ja (Bioanalysis & Biotransformation Research Center, Korea Institute Science & Technology) ;
  • Chung, Bong Chul (Bioanalysis & Biotransformation Research Center, Korea Institute Science & Technology)
  • 이정애 (한국과학기술연구원, 생체대사연구센터) ;
  • 박송자 (한국과학기술연구원, 생체대사연구센터) ;
  • 정봉철 (한국과학기술연구원, 생체대사연구센터)
  • Received : 2004.04.19
  • Accepted : 2004.04.30
  • Published : 2004.06.25

Abstract

A method is described for the analysis of short-chain alkylphenol ethoxylates (APEOs), 4-octylphenol-di-ethoxylate (OP2EO) and 4-nonylphenol-di-ethoxylate (NP2EO), in drinking water or wastewater using reversed phase high-performance liquid chromatography with electrospray ionization mass spectrometry. The solvent system was water and methanol containing $10{\mu}M$ trifluoroacetic acid as an ionization solvent. We acidified 1 L of water samples to less than pH 2 with concentrated $H_2SO_4$ and loaded onto Sep-Pak $C_{18}$, and eluted with acetone. The calibration of OP2EO and NP2EO was performed for the concentration range from 20 to 500 ng/L and the correlation coefficients were 0.999 and 0.990, respectively. The limits of detection were 20 ng/L (OP2EO) and 50 ng/L (NP2EO) at a signal-to-noise ratio of 3. Accuracy and precision of this analytical method were 85.8 ~ 122.1% and 8.2 ~ 18.8%, respectively. The proposed method allowed a sensitive and rapid detection of OP2EO and NP2EO and it could be applied for monitoring of APEOs from environmental samples.

Keywords

alkylphenol ethoxylate;electrospray mass spectrometry

Acknowledgement

Supported by : 과학기술부

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