Determination of alkylphenol ethoxylate in water by high performance liquid chromatography/electrospray ionization/mass spectrometry

HPLC/ESI/MS를 이용한 물 중의 알킬페놀에톡실레이트 분석

  • Lee, Jeongae (Bioanalysis & Biotransformation Research Center, Korea Institute Science & Technology) ;
  • Park, Song-Ja (Bioanalysis & Biotransformation Research Center, Korea Institute Science & Technology) ;
  • Chung, Bong Chul (Bioanalysis & Biotransformation Research Center, Korea Institute Science & Technology)
  • 이정애 (한국과학기술연구원, 생체대사연구센터) ;
  • 박송자 (한국과학기술연구원, 생체대사연구센터) ;
  • 정봉철 (한국과학기술연구원, 생체대사연구센터)
  • Received : 2004.04.19
  • Accepted : 2004.04.30
  • Published : 2004.06.25


A method is described for the analysis of short-chain alkylphenol ethoxylates (APEOs), 4-octylphenol-di-ethoxylate (OP2EO) and 4-nonylphenol-di-ethoxylate (NP2EO), in drinking water or wastewater using reversed phase high-performance liquid chromatography with electrospray ionization mass spectrometry. The solvent system was water and methanol containing $10{\mu}M$ trifluoroacetic acid as an ionization solvent. We acidified 1 L of water samples to less than pH 2 with concentrated $H_2SO_4$ and loaded onto Sep-Pak $C_{18}$, and eluted with acetone. The calibration of OP2EO and NP2EO was performed for the concentration range from 20 to 500 ng/L and the correlation coefficients were 0.999 and 0.990, respectively. The limits of detection were 20 ng/L (OP2EO) and 50 ng/L (NP2EO) at a signal-to-noise ratio of 3. Accuracy and precision of this analytical method were 85.8 ~ 122.1% and 8.2 ~ 18.8%, respectively. The proposed method allowed a sensitive and rapid detection of OP2EO and NP2EO and it could be applied for monitoring of APEOs from environmental samples.


Supported by : 과학기술부


  1. D. Y. Shang, M. G. Ikonomou and R. W. Macdonald, J. Chromatogr. A, 849, 467-482(1999).
  2. S. A. Snyder, T. L. Keith, C. G. Naylor, C. A. Staples and J. P. Giesy, Environ. Toxicol. Chem., 20, 1870-3(2001).
  3. C. Crescenzi, A. Di Corcia and R. Samperi, Anal. Chem., 67, 1797(1995).
  4. M. Ahel and W. Giger, Anal. Chem., 57, 2584(1985).
  5. A. A. Boyd-Boland and J. B. Pawliszyn, Anal. Chem., 68, 1521(1996).
  6. B. Shao, J-Y. Hu and M. Yang, J. Chromatogr. A, 950, 167-174(2002).
  7. T. Reemtsma, Trands in Anal. Chem., 20, 500-517(2001).
  8. M. Petrovic and D. Barcelo, J. Mass Spectrom., 36, 1173-1185(2001).
  9. P. L. Ferguson, C. R. Iden and B. J. Brownawell, Anal. Chem., 72, 4322-4330(2000).
  10. M. Reinhard, N. Goodman, K. E. Mortelmans, Environ. Sci. Technol., 16, 351(1982).
  11. M. J. La Guardia, R. C. Hale, E. Harvey and T. M. Mainor, Environ. Sci. Technol., 35, 4798-4804(2001).
  12. T. Tanghe, G. Devriese, W. Verstraete, Water Res., 32, 2889(1998).
  13. A. Marcomini, B. Pavoni, A. Sfriso and A. A. Orio, Marine Chem., 29, 307(1990).
  14. I. Moreno-Garrido, M. Hampel, L. M. Lubian and J. Blasco, Fresenius J. Anal. Chem., 371, 474-8(2001).
  15. M. Petrovic, A. Diaz, F. Ventura and D. Barcelo, Anal. Chem., 73, 5886-5895(2001).
  16. B. J. Hoffman and L. T. Taylor, J. Chromatogr. Sci., 40, 61-8(2002).
  17. S. Jobling, D. Sheahan, J. A. Osborne, P. Matthiessen, and J. P. Sumpter, Environ. Tixicol. Chem., 15, 194(1996).
  18. A. Cohen, K. Klint, S. Bowadt, P. Persson and J. A. Jonsson, J. Chromatogr. A, 927, 103-110(2001).
  19. C. Sonnenschein, A. M. Soto, J. Steroid Biochem. Mol. Biol., 65, 143(1998).