A study of the metabolites for 7-keto-DHEA-acetate in human urine

뇨시료에 함유된 7-keto-DHEA-acetate의 대사체에 관한 연구 (I)

  • Kim, Yunje (Doping Control Center, Korea Institute of Science and Technology) ;
  • Lee, Jinhee (Doping Control Center, Korea Institute of Science and Technology)
  • 김연제 (한국과학기술연구원 도핑콘트롤센터) ;
  • 이진희 (한국과학기술연구원 도핑콘트롤센터)
  • Received : 2004.06.30
  • Accepted : 2004.07.16
  • Published : 2004.08.25

Abstract

7-keto-dehydroepiandrosterone-acetate (7-keto-DHEA-acetate) is an anabolic steroids, and we studied basically to the metabolites of it after human dosing. We tested the matrix effect from human urine to detect the 7-keto-DHEA-acetate. And LC/ESI/MS and GC/MSD was used to detect the metabolites in dosed urine. We found the some unknown compound from dosed urine (M1, M2, M3, M4 and M5), and from these results, we supposed that these compounds have the more than 3 hydroxyl and/or ketone group. Metabolite M1 was supposed that molecular weight is 302 and 3-,17-diketone and 7-hydroxyl compound (7-OH-androstendione). Metabolite M2 was supposed that the molecular weight was same to M1 and 7-,17-diketone and 3-hydroxyl compound (7-keto-DHEA).

Keywords

7-keto-dehydroepiandrosterone-acetate;metabolites;human dosed urine;metabolite M1;metabolite M2;LC/ESI/MS;GC/MS

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