Structural Studies of Membrane Protein by Solid-state NMR Spectroscopy

고체상 핵자기공명 분광법을 이용한 막단백질의 구조연구

  • Kim, Yongae (Department of Chemistry, Hankuk Univ. of Foreign Studies)
  • 김용애 (한국외국어대학교 화학과)
  • Received : 2004.06.15
  • Accepted : 2004.07.30
  • Published : 2004.10.25

Abstract

Structural studies of membrane proteins, importantly involving interpretation of genomics information, many signaling pathway and major drug target for drug discovery, are having difficulty in characterizing the function using conventional solution nmr spectroscopy and x-ray crystallography because phospholipid bilayers hindered fast tumbling and crystallization. Here, we studied the structure of the pf1 coat protein in oriented phospholipid bilayers by home-built solid-state NMR probe. Bacteriophage pf1 was purified from Paeudomonas Aeruginosa and coat protein of bacteriophage pf1 was isolated from DNA and other proteins.

Keywords

Membrane protein;Solid-state NMR;Home-built solid-state NMR probe;Bacteriophage pf1 coat protein;protein purification

Acknowledgement

Supported by : 한국외국어대학교

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