A study of the metabolites for 7-keto-DHEA-acetate in human urine (II)

뇨시료에 함유된 7-keto-DHEA-acetate의 대사체에 관한 연구 (II)

  • Kim, Yunje (Doping Control Center, Korea Institute of Science and Technology) ;
  • Lee, Jinhee (Doping Control Center, Korea Institute of Science and Technology)
  • 김연제 (한국과학기술연구원 도핑콘트롤센터) ;
  • 이진희 (한국과학기술연구원 도핑콘트롤센터)
  • Received : 2004.08.16
  • Accepted : 2004.09.09
  • Published : 2004.10.25

Abstract

The 7-keto-DHEA-acetate is converted to 7-keto-DHEA, a metabolite of DHEA, and similar to its metabolism. We studied the metabolite M3, M4, and M5 of 7-keto-DHEA-acetate. The estimated molecular weight of M3 and M4 was 304 which were supposed to have more 3 hydroxyl and/or ketone groups. We could know that M3 is the 7-OH-DHEA which has the hydroxyl groups on 3 and 7-carbon and a ketone group on 17-carbon. In case of M4, it is the 7-oxo-diol metabolite which has the hydroxyl groups on 3 and 17-carbon and a ketone group on 7-carbon. The M5 was supposed that the molecular weight is 320 and has the three hydroxyl groups on 3, 6, and 16 carbon and the ketone group on 17-carbon. After dosing, 7-OH-DHEA showed the maximum urine flow in human urine after 5 hr and decreased rapidly. But we could find it until 58 hr why is a higher remaining substance.

Keywords

7-keto-dehydroepiandrosterone-acetate;dosed human urine;metabolite M3;metabolite M4;metabolite M5;LC/ESI/MS;GC/MS

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