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Isolation of Antioxidant and Antibrowning Substance from Chionanthus retusa Leaves

이팝나무 잎으로부터 항산화 및 항갈변물질의 분리

  • Lee, Young-Nam (Division of Applied Life Science, Graduate School,Gyeongsang National University) ;
  • Jeong, Chang-Ho (Division of Applied Life Science, Graduate School,Gyeongsang National University) ;
  • Shim, Ki-Hwan (Institute of Agricultural & Life Sciences, Gyeongsang National University)
  • 이영남 (경상대학교 대학원 응용생명과학부) ;
  • 정창호 (경상대학교 대학원 응용생명과학부) ;
  • 심기환 (경상대학교 농업생명과학연구원)
  • Published : 2004.11.01

Abstract

This study was performed to examine in vitro antioxidative activities such as DPPH radical scavenging activity, reducing power and tyrosinase inhibitory effect of various solvent fractions from Chionanthus retusa leaves. Ethyl acetate fraction showed potent antioxidative activity and tyrosinase inhibitory effect. The active compound was isolated from the butanol fraction by silica gel column chromatography and MPLC. The isolated compound was luteolin-4'-O-glucoside determined by $^1H$, $^{13}C$-NMR and 2D NMR. Compared with several antioxidant compounds, luteolin-4'-O-glucoside exhibited effective DPPH radical scavenging activity and reducing power in a concentration dependent manner. Bioassay with pure luteolin-4'-O-glucoside showed a dose-independent inhibitory effect on L-DOPA oxidation by mushroom tyrosinase and its $IC_{50}$ values were established as 23.2 ${\mu}g/mL$. Therefore, we may suggest that luteolin-4'-O-glucoside can be used as a food additive possessing the potent antioxidative activity and skin-whitening cosmetic material.

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